Table 6.

In vivo efficacy studies in a Leishmania infantum visceral murine model. Parasite burden in liver, spleen and BM was measured by qRT-PCR after 5 or 10 consecutive days of i.p. treatment with free BER and LP-BER at 5, 10 and 15 mg/kg. Fungizone^® (FGZ) i.v. administered at 1 mg/kg was used as positive control. Results expressed as median ± 95% IC (n = 6 per group). Data analyzed using a nonparametric Kruskal–Wallis test, followed by Dunn’s multiple comparison. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.

			Liver		Spleen		BM (Bone Marrow)
	Dose (mg/kg)		Median	95% IC	Median	95% IC	Median	95% IC
5 Days		Control	3.4 × 107	4 × 106–8 × 107	2.8 × 103	1 × 103–5 × 103	5.6 × 105	3 × 105–1 × 106
	5	BER	4.4 × 106	9 × 105–1 × 107	2.5 × 103	6 × 102–2 × 104	8.7 × 105	2 × 105–3 × 106
		LP-BER	3.5 × 107	1 × 107–1 × 108	2.9 × 103	2 × 103–1 × 104	1.1 × 106	2 × 105–4 × 106
	10	BER	4.9 × 107	2 × 107–1 × 108	2.3 × 103	7 × 102–9 × 103	2.0 × 106	8 × 105–3 × 106
		LP-BER	1.7 × 107	7 × 106–5 × 107	2.5 × 103	2 × 103–6 × 103	6.6 × 105	1 × 105–1 × 106
	1	FGZ	8.0 × 105	3 × 105–1 × 107	6.0 × 102	2 × 102–2 × 103	2.3 × 105	2 × 104–5 × 105
10 Days		Control	5.0 × 107	4 × 105–9 × 107	6.1 × 103	2 × 103–1 × 104	5.1 × 105	8 × 104–2 × 106
	15	BER	7.3 × 106	1 × 106–2 × 107	1.1 × 103	4 × 102–3 × 104	7.9 × 105	1 × 105–3 × 106
		LP-BER	5.6 × 104 *	1 × 104–2 × 106	3.2 × 102 **	2 × 102–9 × 102	1.1 × 106	8 × 104–5 × 106
	1	FGZ	1.8 × 102 ***	1–7 × 102	5.2 ****	1–1 × 101	1.3 × 101 *	6–2 × 101