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. 2020 Aug 28;12(9):823. doi: 10.3390/pharmaceutics12090823

Table 1.

List of substances used for the preparation of 1 L simulated body fluid (SBF). Substances were added to deionized water in the order given in the table. Afterwards, the pH of the solution was adjusted to pH 7.4 and the volume was filled up to 1 L using deionized water. Adapted with permission from [33]; Published by Springer Nature, 2015.

Substance Amount (g/L)
NaCl 8.035
NaHCO3 0.355
KCl 0.225
K2HPO4 × 3 H2O 0.231
MgCl2 × 6 H2O 0.311
HCl (1 M) 38 mL
CaCl2 × 2 H2O 0.386
Na2SO4 0.072
Tris 6.118