The genomes of 450 members of Burkholderiaceae, isolated from clinical and environmental sources, were sequenced and assembled as a resource for genome mining. Genomic analysis of the collection has enabled the identification of multiple metabolites and their biosynthetic gene clusters, including the antibiotics gladiolin, icosalide A, enacyloxin, and cepacin A.
ABSTRACT
The genomes of 450 members of Burkholderiaceae, isolated from clinical and environmental sources, were sequenced and assembled as a resource for genome mining. Genomic analysis of the collection has enabled the identification of multiple metabolites and their biosynthetic gene clusters, including the antibiotics gladiolin, icosalide A, enacyloxin, and cepacin A.
ANNOUNCEMENT
Burkholderia is a diverse genus of bacteria and a rich source of antimicrobial compounds (1). Modern natural product discovery is often guided by genome mining (2) and increasingly relies on large genome collections. Therefore, we have sequenced, assembled, and deposited into public databases a substantial collection of genomes of members of Burkholderia and related genera to promote natural product discovery.
Genomic DNA was extracted, using the automated Maxwell 16 instrument with the tissue extraction kit (Promega), from Burkholderia strains and strains of related genera that had been grown in tryptic soy broth overnight at 30°C or 37°C. HiSeq 2000 and HiSeq X Ten instruments, with the associated Illumina HiSeq kits for library preparation, generated 125-bp and 150-bp paired-end reads, respectively. Reads were trimmed using Trim Galore v0.4.2 (3), their quality was assessed using FastQC v0.10.1 (4), and overlapping reads were merged using FLASH v1.2.11 (5). The resulting reads were assembled into contigs using the assembler SPAdes v3.9.1 (6). Pilon v1.2125 (7) identified and corrected misassembled contigs, and contigs representing contaminating DNA were identified with Kraken v0.10.5-beta (8) using the MiniKraken database. In addition, four Burkholderia strains were sequenced with a Pacific Bioscience RS II instrument using genomic DNA prepared with the SMRTbell template preparation kit v2.0. The sequencing was performed in two runs with P4-C2 and P5-C3 chemistries, and the genomes were assembled with SMRT Analysis v2.3.0 as described previously (9). A quality assessment of the final genome assemblies was performed with QUAST v4.4 (10) (Table 1). Genomic identification was performed by average nucleotide identity (ANI) analyses with fastANI v1.2 (11) and pyani v0.2.9 (ANI using MUMmer [ANIm] to align input sequences) (12), using a ≥95% species delineation threshold (11). The NCBI Prokaryotic Genome Annotation Pipeline (PGAP) v4.12 was used for genome annotation (13). Bioinformatics were supported by the Cloud Infrastructure for Microbial Bioinformatics (CLIMB) (14). Default software parameters were used except where otherwise noted.
TABLE 1.
Strain information and genome assembly statistics for the Burkholderiaceae genome collection
| Species | Strain | Isolation source | Geographic location | Read BioSample accession no. | Assembly BioSample accession no. | Assembly GenBank accession no. | Coverage (×) (technology) | Genome size (Mbp) | GC content (%) | N50 (bp) |
|---|---|---|---|---|---|---|---|---|---|---|
| Burkholderia ambifaria | BCC0118 | Clinical, sputum | USA | ERS784989 | ERS4267623 | GCA_902829755 | 81 (Illumina) | 7.50 | 66.82 | 273,879 |
| Burkholderia ambifaria | BCC0191 | Environmental, soil | USA | ERS784799 | ERS4267624 | GCA_902829465 | 82 (Illumina) | 7.58 | 66.56 | 302,202 |
| Burkholderia ambifaria | BCC0192 | Environmental | USA | ERS785047 | ERS4267625 | GCA_902829785 | 73 (Illumina) | 7.40 | 66.73 | 277,504 |
| Burkholderia ambifaria | BCC0197 | Environmental, Sesbania exaltata leaves | ERS785076 | ERS4267626 | GCA_902829415 | 71 (Illumina) | 7.38 | 66.67 | 386,867 | |
| Burkholderia ambifaria | BCC0250 | Clinical, sputum | Australia | ERS784819 | ERS4267627 | GCA_902829765 | 86 (Illumina) | 7.36 | 66.84 | 396,147 |
| Burkholderia ambifaria | BCC0267 | Clinical, cystic fibrosis patient | Australia | ERS784835 | ERS4267628 | GCA_902829645 | 80 (Illumina) | 7.36 | 66.76 | 784,052 |
| Burkholderia ambifaria | BCC0284 | Environmental, corn | USA | ERS1328916 | ERS4267629 | GCA_902829725 | 59 (Illumina) | 7.47 | 66.68 | 423,851 |
| Burkholderia ambifaria | BCC0316 | Environmental, soil | USA | ERS784850 | ERS4267630 | GCA_902829685 | 83 (Illumina) | 7.64 | 66.54 | 292,321 |
| Burkholderia ambifaria | BCC0338 | Environmental, corn roots | USA | ERS1371637 | ERS4267631 | GCA_902829825 | 90 (Illumina) | 7.45 | 66.70 | 514,723 |
| Burkholderia ambifaria | BCC0399 | Environmental | USA | ERS785037 | ERS4267632 | GCA_902829775 | 77 (Illumina) | 7.40 | 66.76 | 407,605 |
| Burkholderia ambifaria | BCC0410 | Environmental, maize | Italy | ERS784866 | ERS4267633 | GCA_902829705 | 93 (Illumina) | 7.38 | 66.78 | 849,830 |
| Burkholderia ambifaria | BCC0423 | Environmental, maize | Italy | ERS1336067 | ERS4267634 | GCA_902829805 | 73 (Illumina) | 7.47 | 66.76 | 382,759 |
| Burkholderia ambifaria | BCC0477 | Clinical, cystic fibrosis patient | USA | ERS784882 | ERS4267635 | GCA_902829695 | 99 (Illumina) | 7.81 | 66.47 | 255,598 |
| Burkholderia ambifaria | BCC0478 | Clinical, cystic fibrosis patient | USA | ERS784897 | ERS4267636 | GCA_902829665 | 109 (Illumina) | 7.24 | 66.82 | 601,161 |
| Burkholderia ambifaria | BCC0480 | Environmental, soil | USA | ERS784913 | ERS4267637 | GCA_902829735 | 113 (Illumina) | 7.84 | 66.57 | 570,779 |
| Burkholderia ambifaria | BCC1041 | Environmental | Italy | ERS784930 | ERS4267638 | GCA_902829815 | 104 (Illumina) | 7.51 | 66.70 | 469,567 |
| Burkholderia ambifaria | BCC1048 | Environmental | Italy | ERS784886 | ERS4267639 | GCA_902829675 | 70 (Illumina) | 7.51 | 66.70 | 469,249 |
| Burkholderia ambifaria | BCC1052 | Environmental | Italy | ERS1371632 | ERS4267640 | GCA_902829435 | 72 (Illumina) | 7.33 | 66.81 | 397,259 |
| Burkholderia ambifaria | BCC1062 | Environmental | Italy | ERS1328829 | ERS4267641 | GCA_902829655 | 65 (Illumina) | 7.45 | 66.69 | 382,455 |
| Burkholderia ambifaria | BCC1065 | Environmental | Italy | ERS784959 | ERS4267642 | GCA_902829835 | 77 (Illumina) | 7.31 | 66.79 | 605,534 |
| Burkholderia ambifaria | BCC1066 | Environmental | Italy | ERS784800 | ERS4267643 | GCA_902829905 | 87 (Illumina) | 7.32 | 66.78 | 381,533 |
| Burkholderia ambifaria | BCC1072 | Environmental | Italy | ERS1328913 | ERS4267644 | GCA_902830005 | 66 (Illumina) | 7.44 | 66.75 | 397,405 |
| Burkholderia ambifaria | BCC1080 | Environmental | Italy | ERS1371635 | ERS4267645 | GCA_902829995 | 84 (Illumina) | 7.29 | 66.76 | 383,339 |
| Burkholderia ambifaria | BCC1083 | Environmental | Italy | ERS1328835 | ERS4267646 | GCA_902829965 | 63 (Illumina) | 7.30 | 66.76 | 373,328 |
| Burkholderia ambifaria | BCC1086 | Environmental | Italy | ERS1328827 | ERS4267647 | GCA_902829915 | 70 (Illumina) | 7.58 | 66.72 | 395,516 |
| Burkholderia ambifaria | BCC1088 | Environmental | Italy | ERS1371633 | ERS4267648 | GCA_902829875 | 87 (Illumina) | 7.48 | 66.70 | 397,405 |
| Burkholderia ambifaria | BCC1090 | Environmental | Italy | ERS1328833 | ERS4267649 | GCA_902829975 | 68 (Illumina) | 7.33 | 66.76 | 340,920 |
| Burkholderia ambifaria | BCC1092 | Environmental | Italy | ERS784808 | ERS4267650 | GCA_902829935 | 77 (Illumina) | 7.38 | 66.78 | 849,686 |
| Burkholderia ambifaria | BCC1093 | Environmental | Italy | ERS784821 | ERS4267651 | GCA_902829945 | 81 (Illumina) | 7.40 | 66.76 | 427,669 |
| Burkholderia ambifaria | BCC1095 | Environmental | Italy | ERS784837 | ERS4267652 | GCA_902829885 | 84 (Illumina) | 7.40 | 66.76 | 407,577 |
| Burkholderia ambifaria | BCC1098 | Environmental | Italy | ERS784852 | ERS4267653 | GCA_902829895 | 83 (Illumina) | 7.38 | 66.78 | 849,506 |
| Burkholderia ambifaria | BCC1100 | Environmental | Italy | ERS784868 | ERS4267654 | GCA_902830015 | 80 (Illumina) | 7.40 | 66.76 | 407,581 |
| Burkholderia ambifaria | BCC1103 | Environmental | Italy | ERS784884 | ERS4267655 | GCA_902829985 | 86 (Illumina) | 7.40 | 66.76 | 427,387 |
| Burkholderia ambifaria | BCC1105 | Environmental | Italy | ERS1371636 | ERS4267656 | GCA_902829845 | 97 (Illumina) | 6.30 | 66.77 | 1,592,784 |
| Burkholderia ambifaria | BCC1107 | Environmental | Italy | ERS784899 | ERS4267657 | GCA_902829855 | 88 (Illumina) | 7.40 | 66.76 | 374,059 |
| Burkholderia ambifaria | BCC1212 | Environmental, rhizosphere | USA | ERS1371638 | ERS4267658 | GCA_902829955 | 82 (Illumina) | 7.61 | 66.44 | 358,611 |
| Burkholderia ambifaria | BCC1213 | Environmental | USA | ERS1328957 | ERS4267659 | GCA_902829925 | 65 (Illumina) | 7.47 | 66.70 | 425,516 |
| Burkholderia ambifaria | BCC1214 | Environmental | USA | ERS784915 | ERS4267660 | GCA_902829595 | 102 (Illumina) | 7.61 | 66.40 | 270,842 |
| Burkholderia ambifaria | BCC1216 | Environmental | USA | ERS1328918 | ERS4267661 | GCA_902829865 | 67 (Illumina) | 7.46 | 66.69 | 234,327 |
| Burkholderia ambifaria | BCC1218 | Environmental | USA | ERS784932 | ERS4267662 | GCA_902830025 | 88 (Illumina) | 7.46 | 66.67 | 348,377 |
| Burkholderia ambifaria | BCC1220 | Environmental | USA | ERS1328837 | ERS4267663 | GCA_902830035 | 65 (Illumina) | 7.64 | 66.39 | 393,412 |
| Burkholderia ambifaria | BCC1223 | Environmental | USA | ERS784947 | ERS4267664 | GCA_902830105 | 104 (Illumina) | 7.61 | 66.40 | 267,383 |
| Burkholderia ambifaria | BCC1224 | Environmental, maize | USA | ERS1328836 | ERS4267665 | GCA_902829395 | 51 (Illumina) | 8.46 | 63.24 | 317,020 |
| Burkholderia ambifaria | BCC1228 | Environmental, maize | USA | ERS1328832 | ERS4267666 | GCA_902830065 | 62 (Illumina) | 7.38 | 66.73 | 558,864 |
| Burkholderia ambifaria | BCC1229 | Environmental, maize | USA | ERS1371639 | ERS4267667 | GCA_902830045 | 65 (Illumina) | 7.51 | 66.70 | 298,531 |
| Burkholderia ambifaria | BCC1233 | Environmental, maize | USA | ERS1328917 | ERS4267668 | GCA_902830075 | 57 (Illumina) | 7.96 | 66.24 | 390,415 |
| Burkholderia ambifaria | BCC1236 | Environmental, maize | USA | ERS1328839 | ERS4267669 | GCA_902830095 | 64 (Illumina) | 7.63 | 66.60 | 358,767 |
| Burkholderia ambifaria | BCC1237 | Environmental, maize | USA | ERS1371640 | ERS4267670 | GCA_902830055 | 66 (Illumina) | 7.34 | 66.84 | 420,767 |
| Burkholderia ambifaria | BCC1240 | Environmental, maize | USA | ERS1328914 | ERS4267671 | GCA_902830085 | 57 (Illumina) | 7.40 | 66.77 | 503,900 |
| Burkholderia ambifaria | BCC1241 | Environmental, maize | USA | ERS784961 | ERS4267672 | GCA_902830175 | 78 (Illumina) | 7.68 | 66.49 | 288,780 |
| Burkholderia ambifaria | BCC1246 | Environmental, maize | USA | ERS1328834 | ERS4267673 | GCA_902830205 | 69 (Illumina) | 7.48 | 66.64 | 450,462 |
| Burkholderia ambifaria | BCC1248 | Environmental, maize | USA | ERS784801 | ERS4267674 | GCA_902830135 | 87 (Illumina) | 8.03 | 66.48 | 241,997 |
| Burkholderia ambifaria | BCC1249 | Environmental, maize | USA | ERS1371634 | ERS4267675 | GCA_902830255 | 97 (Illumina) | 7.58 | 66.75 | 447,443 |
| Burkholderia ambifaria | BCC1252 | Environmental, maize | USA | ERS784809 | ERS4267676 | GCA_902830125 | 83 (Illumina) | 7.42 | 66.71 | 254,705 |
| Burkholderia ambifaria | BCC1256 | Environmental, maize | USA | ERS784823 | ERS4267677 | GCA_902830165 | 86 (Illumina) | 7.54 | 66.60 | 281,063 |
| Burkholderia ambifaria | BCC1258 | Environmental, maize | USA | ERS1371641 | ERS4267678 | GCA_902829475 | 81 (Illumina) | 7.60 | 66.61 | 272,850 |
| Burkholderia ambifaria | BCC1259 | Environmental, maize | USA | ERS784838 | ERS4267679 | GCA_902830225 | 78 (Illumina) | 7.63 | 66.39 | 431,727 |
| Burkholderia ambifaria | BCC1265 | Environmental | USA | ERS784854 | ERS4267680 | GCA_902830115 | 79 (Illumina) | 7.62 | 66.44 | 335,885 |
| Burkholderia ambifaria | BCC1270 | Environmental, maize | USA | ERS784870 | ERS4267681 | GCA_902830155 | 73 (Illumina) | 7.60 | 66.61 | 315,858 |
| Burkholderia multivorans | BCC1599 | Environmental | ERS1371642 | ERS4267682 | GCA_902833025 | 87 (Illumina) | 6.74 | 67.15 | 248,547 | |
| Burkholderia sp. | BCC1630 | Environmental | Malaysia | ERS1328847 | ERS4267683 | GCA_902833165 | 75 (Illumina) | 7.62 | 66.03 | 201,973 |
| Burkholderia sp. | BCC1638 | Environmental | Malaysia | ERS1328851 | ERS4267684 | GCA_902833195 | 89 (Illumina) | 6.96 | 66.61 | 206,632 |
| Burkholderia anthina | BCC0036 | Clinical, cystic fibrosis patient | UK | ERS785067 | ERS4267685 | GCA_902830145 | 69 (Illumina) | 7.72 | 66.49 | 216,836 |
| Burkholderia anthina | BCC0403 | Environmental, river | USA | ERS784934 | ERS4267686 | GCA_902830325 | 76 (Illumina) | 8.15 | 66.41 | 375,294 |
| Burkholderia anthina | BCC0485 | Clinical, cystic fibrosis patient | USA | ERS1328919 | ERS4267687 | GCA_902829375 | 49 (Illumina) | 9.30 | 61.27 | 152,611 |
| Burkholderia anthina | BCC0518 | Environmental, soil | UK | ERS1328922 | ERS4267688 | GCA_902830485 | 66 (Illumina) | 8.28 | 66.46 | 220,529 |
| Burkholderia anthina | BCC0634 | Environmental, soil rhizosphere | USA | ERS1328841 | ERS4267689 | GCA_902830375 | 87 (Illumina) | 7.94 | 65.74 | 168,505 |
| Burkholderia anthina | BCC0635 | Environmental, rhizosphere | ERS1333586 | ERS4267690 | GCA_902830445 | 89 (Illumina) | 7.76 | 66.03 | 194,880 | |
| Burkholderia anthina | BCC0636 | Environmental, Carludovica palmata rhizosphere | UK | ERS1328842 | ERS4267691 | GCA_902830345 | 95 (Illumina) | 7.76 | 66.03 | 195,048 |
| Burkholderia anthina | BCC0637 | Environmental, soil | ERS1328920 | ERS4267692 | GCA_902830315 | 101 (Illumina) | 7.30 | 66.36 | 205,801 | |
| Burkholderia anthina | BCC0638 | Environmental, soil | ERS1328843 | ERS4267693 | GCA_902829625 | 102 (Illumina) | 7.69 | 66.33 | 147,404 | |
| Burkholderia anthina | LMG 20980 | Environmental, soil rhizosphere | USA | ERS1371643 | ERS4267694 | GCA_902830395 | 71 (Illumina) | 7.63 | 66.74 | 336,735 |
| Burkholderia anthina | BCC1349 | Clinical | ERS1328844 | ERS4267695 | GCA_902830335 | 76 (Illumina) | 8.61 | 66.32 | 444,914 | |
| Burkholderia stagnalis | BCC1350 | Environmental | ERS784963 | ERS4267696 | GCA_902833065 | 83 (Illumina) | 8.37 | 67.13 | 251,925 | |
| Burkholderia anthina | BCC1351 | Clinical | ERS1328921 | ERS4267697 | GCA_902829335 | 94 (Illumina) | 7.48 | 66.21 | 196,378 | |
| Burkholderia anthina | BCC1358 | Clinical | ERS1371644 | ERS4267698 | GCA_902830365 | 68 (Illumina) | 9.16 | 66.08 | 173,669 | |
| Burkholderia anthina | BCC1363 | Environmental | ERS1336069 | ERS4267699 | GCA_902830415 | 85 (Illumina) | 7.28 | 66.32 | 170,879 | |
| Burkholderia arboris | BCC0049 | Clinical, non-cystic fibrosis patient | France | ERS785032 | ERS4267700 | GCA_902830425 | 64 (Illumina) | 8.28 | 66.77 | 418,964 |
| Burkholderia cenocepacia | J2315 | Clinical, respiratory sample | UK | ERS784840 | ERS4267701 | GCA_902830575 | 82 (Illumina) | 7.91 | 66.99 | 221,803 |
| Burkholderia cenocepacia | BCC0016 | Clinical, sputum | Canada | ERS784856 | ERS4267702 | GCA_902830355 | 81 (Illumina) | 7.89 | 67.01 | 175,882 |
| Burkholderia cenocepacia | BCC0017 | Clinical, sputum | Canada | ERS785082 | ERS4267703 | GCA_902830435 | 79 (Illumina) | 8.10 | 67.17 | 243,601 |
| Burkholderia cepacia | BCC0018 | Clinical, cystic fibrosis patient | UK | ERS785027 | ERS4267704 | GCA_902830685 | 59 (Illumina) | 8.54 | 66.76 | 891,303 |
| Burkholderia cenocepacia | BCC0020 | Clinical, cystic fibrosis patient | Australia | ERS784871 | ERS4267705 | GCA_902830475 | 88 (Illumina) | 7.30 | 66.88 | 536,526 |
| Burkholderia cenocepacia | BCC0048 | Clinical, non-cystic fibrosis patient | France | ERS784998 | ERS4267706 | GCA_902830385 | 64 (Illumina) | 8.32 | 66.91 | 293,098 |
| Burkholderia sp. | BCC0097 | Clinical, respiratory sample | Canada | ERS784888 | ERS4267707 | GCA_902832985 | 65 (Illumina) | 7.94 | 66.71 | 613,508 |
| Burkholderia cenocepacia | BCC0107 | Clinical, sputum | Canada | ERS785010 | ERS4267708 | GCA_902830455 | 68 (Illumina) | 8.19 | 67.10 | 293,110 |
| Burkholderia cenocepacia | BCC0127 | Clinical, sputum | USA | ERS785018 | ERS4267709 | GCA_902830405 | 71 (Illumina) | 7.73 | 66.79 | 420,157 |
| Burkholderia contaminans | BCC0132 | Clinical, sputum | Canada | ERS785057 | ERS4267710 | GCA_902830775 | 64 (Illumina) | 8.46 | 66.61 | 151,177 |
| Burkholderia cenocepacia | BCC0138 | Clinical | Canada | ERS785048 | ERS4267711 | GCA_902830465 | 67 (Illumina) | 7.99 | 67.24 | 228,664 |
| Burkholderia cenocepacia | BCC0160 | Clinical | USA | ERS784903 | ERS4267712 | GCA_902830505 | 81 (Illumina) | 6.98 | 66.93 | 421,783 |
| Burkholderia cenocepacia | BCC0165 | Clinical, cystic fibrosis patient | Belgium | ERS785066 | ERS4267713 | GCA_902830495 | 73 (Illumina) | 7.52 | 66.96 | 1,954,875 |
| Burkholderia cenocepacia | BCC0199 | Environmental | USA | ERS785064 | ERS4267714 | GCA_902830545 | 72 (Illumina) | 7.97 | 66.66 | 275,726 |
| Burkholderia cenocepacia | BCC0202 | Environmental | USA | ERS784992 | ERS4267715 | GCA_902830535 | 84 (Illumina) | 7.61 | 66.87 | 596,170 |
| Burkholderia cenocepacia | BCC0313 | Clinical | ERS784920 | ERS4267716 | GCA_902830515 | 77 (Illumina) | 8.62 | 65.53 | 301,741 | |
| Burkholderia contaminans | BCC0320 | Environmental, radish | Mexico | ERS784936 | ERS4267717 | GCA_902830835 | 84 (Illumina) | 8.33 | 66.35 | 606,600 |
| Burkholderia cenocepacia | BCC0451 | Environmental, maize | ERS784950 | ERS4267718 | GCA_902830565 | 91 (Illumina) | 7.52 | 66.92 | 1,207,947 | |
| Burkholderia sp. | BCC0506 | Clinical, cystic fibrosis patient | Italy | ERS784858 | ERS4267719 | GCA_902833145 | 90 (Illumina) | 7.29 | 67.22 | 506,111 |
| Burkholderia sp. | BCC0801 | Clinical, cystic fibrosis patient | Argentina | ERS784873 | ERS4267720 | GCA_902833175 | 88 (Illumina) | 7.50 | 67.06 | 298,805 |
| Burkholderia cenocepacia | BCC0999 | Environmental | ERS784803 | ERS4267721 | GCA_902830585 | 96 (Illumina) | 7.62 | 66.81 | 225,632 | |
| Burkholderia cenocepacia | BCC1028 | Environmental | ERS784811 | ERS4267722 | GCA_902830605 | 91 (Illumina) | 7.59 | 66.87 | 470,293 | |
| Burkholderia sp. | BCC1208 | Environmental, lupine shoot endophyte | Australia | ERS784827 | ERS4267723 | GCA_902833095 | 91 (Illumina) | 7.38 | 66.96 | 490,408 |
| Burkholderia cenocepacia | BCC1266 | Environmental | ERS785055 | ERS4267724 | GCA_902830595 | 65 (Illumina) | 7.92 | 66.65 | 259,121 | |
| Burkholderia cenocepacia | BCC1322 | Environmental/industrial | ERS784842 | ERS4267725 | GCA_902829445 | 82 (Illumina) | 7.94 | 66.75 | 571,121 | |
| Burkholderia cenocepacia | BCC1556 | Environmental/industrial | UK | ERS785001 | ERS4267726 | GCA_902830525 | 78 (Illumina) | 7.21 | 67.13 | 1,136,705 |
| Burkholderia cepacia | BCC1631 | Environmental | ERS1328961 | ERS4267727 | GCA_902830715 | 71 (Illumina) | 8.72 | 66.64 | 276,956 | |
| Burkholderia cepacia | BCC1632 | Environmental | ERS1328848 | ERS4267728 | GCA_902830785 | 83 (Illumina) | 8.83 | 66.59 | 273,961 | |
| Burkholderia cepacia | BCC1634 | Environmental | ERS1328849 | ERS4267729 | GCA_902830765 | 90 (Illumina) | 7.59 | 66.62 | 403,579 | |
| Burkholderia cepacia | BCC1636 | Environmental | ERS1328850 | ERS4267730 | GCA_902829425 | 88 (Illumina) | 8.48 | 66.70 | 266,835 | |
| Burkholderia cepacia | BCC1641 | Environmental | Malaysia | ERS1328925 | ERS4267731 | GCA_902830805 | 80 (Illumina) | 8.72 | 66.64 | 256,085 |
| Burkholderia sp. | BCC1644 | Environmental | ERS1371646 | ERS4267732 | GCA_902833225 | 71 (Illumina) | 8.25 | 66.71 | 390,260 | |
| Burkholderia cenocepacia | BCC1718 | Clinical | ERS785000 | ERS4267733 | GCA_902830555 | 70 (Illumina) | 7.70 | 66.81 | 543,487 | |
| Burkholderia cepacia | BCC0001 | Environmental, onion | ERS784996 | ERS4267734 | GCA_902830625 | 67 (Illumina) | 8.51 | 66.65 | 467,216 | |
| Burkholderia cepacia | BCC0002 | Environmental, forest soil | Trinidad and Tobago | ERS784890 | ERS4267735 | GCA_902830635 | 68 (Illumina) | 8.54 | 66.76 | 891,307 |
| Burkholderia cepacia | BCC0003 | Clinical, cystic fibrosis patient | Australia | ERS784995 | ERS4267736 | GCA_902830665 | 68 (Illumina) | 8.51 | 66.29 | 289,400 |
| Burkholderia cepacia | BCC0196 | Environmental, grass | ERS784922 | ERS4267737 | GCA_902830615 | 75 (Illumina) | 8.53 | 66.58 | 453,915 | |
| Burkholderia ambifaria | BCC0200 | Environmental | ERS785045 | ERS4267738 | GCA_902829565 | 76 (Illumina) | 7.63 | 66.55 | 331,719 | |
| Burkholderia cepacia | BCC0233 | Environmental | ERS785054 | ERS4267739 | GCA_902830645 | 66 (Illumina) | 8.82 | 66.50 | 775,678 | |
| Burkholderia cepacia | BCC0256 | Clinical, sputum | New Zealand | ERS784905 | ERS4267740 | GCA_902830655 | 59 (Illumina) | 9.19 | 66.32 | 987,550 |
| Burkholderia cepacia | BCC0367 | Clinical | ERS784937 | ERS4267741 | GCA_902830675 | 86 (Illumina) | 8.68 | 66.51 | 796,538 | |
| Burkholderia cepacia | BCC0970 | Environmental | ERS784952 | ERS4267742 | GCA_902830695 | 77 (Illumina) | 8.37 | 66.76 | 428,681 | |
| Burkholderia cepacia | BCC0974 | Environmental | ERS784967 | ERS4267743 | GCA_902830705 | 86 (Illumina) | 8.59 | 66.74 | 450,214 | |
| Burkholderia cepacia | BCC0977 | Environmental | ERS784804 | ERS4267744 | GCA_902830755 | 106 (Illumina) | 6.93 | 67.06 | 573,127 | |
| Burkholderia cepacia | BCC1058 | Environmental | ERS784813 | ERS4267745 | GCA_902830745 | 81 (Illumina) | 8.37 | 66.76 | 505,601 | |
| Burkholderia cepacia | BCC1273 | ERS784829 | ERS4267746 | GCA_902830735 | 80 (Illumina) | 8.51 | 66.80 | 984,555 | ||
| Burkholderia sp. | BCC1640 | Environmental | Malaysia | ERS1328852 | ERS4267747 | GCA_902833125 | 92 (Illumina) | 7.20 | 66.40 | 186,730 |
| Burkholderia sp. | BCC0044 | Clinical, cystic fibrosis patient | UK | ERS785043 | ERS4267748 | GCA_902832955 | 70 (Illumina) | 8.04 | 66.82 | 1,287,212 |
| Burkholderia sp. | BCC0322 | Clinical, cystic fibrosis patient | UK | ERS784908 | ERS4267749 | GCA_902833045 | 71 (Illumina) | 7.75 | 66.27 | 527,417 |
| Burkholderia sp. | BCC0397 | Clinical | ERS784940 | ERS4267750 | GCA_902832865 | 71 (Illumina) | 8.16 | 66.81 | 442,581 | |
| Burkholderia sp. | BCC0398 | Environmental | ERS785051 | ERS4267751 | GCA_902833245 | 65 (Illumina) | 8.01 | 66.54 | 387,212 | |
| Burkholderia sp. | BCC0405 | Environmental | ERS784923 | ERS4267752 | GCA_902833075 | 71 (Illumina) | 7.98 | 66.08 | 309,371 | |
| Burkholderia sp. | BCC0419 | Environmental, maize | ERS784958 | ERS4267753 | GCA_902830285 | 66 (Illumina) | 8.18 | 66.71 | 1,058,720 | |
| Burkholderia sp. | BCC1047 | Environmental | ERS784973 | ERS4267754 | GCA_902833155 | 83 (Illumina) | 7.79 | 66.79 | 153,691 | |
| Burkholderia pseudomultivorans | BCC1191 | Clinical | ERS785049 | ERS4267755 | GCA_902832925 | 73 (Illumina) | 7.55 | 67.02 | 243,992 | |
| Burkholderia contaminans | BCC0123 | Clinical, sputum | USA | ERS785009 | ERS4267756 | GCA_902830725 | 60 (Illumina) | 8.77 | 66.35 | 548,891 |
| Burkholderia contaminans | BCC0254 | Clinical, sputum | Australia | ERS784845 | ERS4267757 | GCA_902830865 | 79 (Illumina) | 8.35 | 66.00 | 564,071 |
| Burkholderia contaminans | BCC0339 | Clinical | ERS784860 | ERS4267758 | GCA_902830845 | 76 (Illumina) | 8.29 | 66.39 | 1,070,961 | |
| Burkholderia contaminans | BCC1315 | Environmental/industrial | ERS784875 | ERS4267759 | GCA_902830875 | 75 (Illumina) | 8.02 | 66.62 | 652,822 | |
| Burkholderia diffusa | BCC0106 | Clinical, cystic fibrosis patient throat | Canada | ERS785072 | ERS4267760 | GCA_902830825 | 72 (Illumina) | 7.80 | 65.92 | 283,477 |
| Burkholderia diffusa | BCC0109 | Clinical, respiratory sample | Canada | ERS785028 | ERS4267761 | GCA_902830855 | 76 (Illumina) | 7.23 | 66.15 | 216,537 |
| Burkholderia diffusa | LMG 29043 | Environmental, soil | USA | ERS4267762 | ERS4267762 | GCA_902830815 | 180 (Illumina) | 7.15 | 66.50 | 236,915 |
| Burkholderia dolosa | BCC0306 | Clinical | USA | ERS785034 | ERS4267763 | GCA_902829575 | 75 (Illumina) | 6.50 | 67.06 | 519,080 |
| Burkholderia gladioli | BCC0238 | Clinical, sputum | USA | ERS333128 | ERS4267764 | GCA_902830885 | 63 (Illumina) | 8.55 | 68.00 | 479,899 |
| Burkholderia gladioli | BCC0252 | Clinical, sputum | Australia | ERS784955 | ERS4267765 | GCA_902830905 | 89 (Illumina) | 8.22 | 68.09 | 289,654 |
| Burkholderia gladioli | BCC0507 | Clinical, cystic fibrosis patient | Italy | ERS784970 | ERS4267766 | GCA_902830895 | 92 (Illumina) | 8.36 | 68.13 | 335,266 |
| Burkholderia gladioli | BCC0771 | Environmental, Gladiolus sp. | USA | ERS784806 | ERS4267767 | GCA_902830915 | 80 (Illumina) | 8.80 | 67.72 | 967,784 |
| Burkholderia gladioli | BCC1317 | Environmental/industrial | UK | ERS785017 | ERS4267768 | GCA_902830925 | 59 (Illumina) | 8.77 | 67.93 | 552,562 |
| Burkholderia gladioli | BCC1619 | Clinical | USA | ERS784817 | ERS4267769 | GCA_902830945 | 89 (Illumina) | 7.88 | 68.13 | 868,935 |
| Burkholderia gladioli | BCC1620 | Clinical | USA | ERS784832 | ERS4267770 | GCA_902830985 | 80 (Illumina) | 8.19 | 68.09 | 416,357 |
| Burkholderia gladioli | BCC1621 | Clinical | USA | ERS784849 | ERS4267771 | GCA_902830995 | 75 (Illumina) | 8.48 | 67.95 | 434,143 |
| Burkholderia gladioli | BCC1622 | Clinical | USA | ERS784864 | ERS4267772 | GCA_902830955 | 79 (Illumina) | 8.41 | 68.05 | 615,150 |
| Burkholderia gladioli | BCC1623 | Clinical | USA | ERS784879 | ERS4267773 | GCA_902830935 | 75 (Illumina) | 8.44 | 67.87 | 365,793 |
| Burkholderia gladioli | BCC1624 | Clinical | USA | ERS784895 | ERS4267774 | GCA_902830975 | 81 (Illumina) | 7.88 | 68.14 | 430,243 |
| Burkholderia gladioli | BCC1645 | Environmental, Allium cepa bulb | Australia | ERS784911 | ERS4267775 | GCA_902830965 | 73 (Illumina) | 8.61 | 67.87 | 383,325 |
| Burkholderia gladioli | BCC1646 | Environmental, Allium cepa | India | ERS784928 | ERS4267776 | GCA_902831005 | 78 (Illumina) | 8.39 | 67.96 | 286,552 |
| Burkholderia gladioli | BCC1647 | Environmental, Gladiolus sp. | USA | ERS784943 | ERS4267777 | GCA_902831075 | 73 (Illumina) | 8.88 | 67.70 | 561,180 |
| Burkholderia gladioli | BCC1648 | Environmental | USA | ERS784957 | ERS4267778 | GCA_902831035 | 79 (Illumina) | 8.62 | 67.87 | 310,631 |
| Burkholderia gladioli | BCC1649 | Environmental, Gladiolus sp. | Zimbabwe | ERS784812 | ERS4267779 | GCA_902831025 | 67 (Illumina) | 8.25 | 68.30 | 554,529 |
| Burkholderia gladioli | BCC1650 | Environmental, poisoned bongkrek | Indonesia | ERS784828 | ERS4267780 | GCA_902831015 | 67 (Illumina) | 8.10 | 68.24 | 708,245 |
| Burkholderia gladioli | BCC1651 | Clinical | USA | ERS784844 | ERS4267781 | GCA_902829385 | 68 (Illumina) | 7.94 | 68.29 | 595,470 |
| Burkholderia gladioli | BCC1661 | Clinical | USA | ERS784861 | ERS4267782 | GCA_902831055 | 63 (Illumina) | 8.33 | 68.06 | 632,785 |
| Burkholderia gladioli | BCC1662 | Clinical | USA | ERS784878 | ERS4267783 | GCA_902831065 | 65 (Illumina) | 8.51 | 68.05 | 410,006 |
| Burkholderia gladioli | BCC1663 | Clinical | USA | ERS784894 | ERS4267784 | GCA_902831085 | 82 (Illumina) | 8.51 | 68.05 | 410,015 |
| Burkholderia gladioli | BCC1664 | Clinical | USA | ERS784910 | ERS4267785 | GCA_902831045 | 62 (Illumina) | 8.51 | 68.06 | 378,407 |
| Burkholderia gladioli | BCC1665 | Clinical | USA | ERS784925 | ERS4267786 | GCA_902831105 | 72 (Illumina) | 8.33 | 68.06 | 601,921 |
| Burkholderia gladioli | BCC1666 | Clinical | USA | ERS784942 | ERS4267787 | GCA_902831115 | 70 (Illumina) | 8.51 | 68.05 | 408,038 |
| Burkholderia gladioli | BCC1667 | Clinical | UK | ERS784960 | ERS4267788 | GCA_902831095 | 71 (Illumina) | 8.49 | 67.84 | 427,447 |
| Burkholderia gladioli | BCC1668 | Clinical | UK | ERS784974 | ERS4267789 | GCA_902831165 | 75 (Illumina) | 8.46 | 67.97 | 603,154 |
| Burkholderia gladioli | BCC1669 | Clinical | UK | ERS784982 | ERS4267790 | GCA_902831175 | 78 (Illumina) | 8.18 | 68.14 | 485,051 |
| Burkholderia gladioli | BCC1670 | Clinical | UK | ERS784814 | ERS4267791 | GCA_902829325 | 68 (Illumina) | 8.29 | 68.03 | 309,426 |
| Burkholderia gladioli | BCC1671 | Clinical | UK | ERS784830 | ERS4267792 | GCA_902831225 | 62 (Illumina) | 8.36 | 68.09 | 369,886 |
| Burkholderia gladioli | BCC1672 | Clinical | UK | ERS784846 | ERS4267793 | GCA_902831265 | 63 (Illumina) | 8.28 | 68.03 | 287,015 |
| Burkholderia gladioli | BCC1674 | Clinical | UK | ERS784994 | ERS4267794 | GCA_902831195 | 68 (Illumina) | 8.44 | 67.87 | 356,821 |
| Burkholderia gladioli | BCC1675 | Clinical | USA | ERS785062 | ERS4267795 | GCA_902831215 | 70 (Illumina) | 8.09 | 68.09 | 936,495 |
| Burkholderia gladioli | BCC1676 | Clinical | USA | ERS784863 | ERS4267796 | GCA_902831315 | 70 (Illumina) | 7.99 | 68.26 | 467,101 |
| Burkholderia gladioli | BCC1677 | Clinical | USA | ERS785071 | ERS4267797 | GCA_902831155 | 75 (Illumina) | 8.11 | 68.25 | 632,433 |
| Burkholderia gladioli | BCC1678 | Clinical | USA | ERS784880 | ERS4267798 | GCA_902831285 | 71 (Illumina) | 7.83 | 68.04 | 235,084 |
| Burkholderia gladioli | BCC1679 | Clinical | USA | ERS784896 | ERS4267799 | GCA_902831135 | 73 (Illumina) | 8.12 | 68.09 | 272,665 |
| Burkholderia gladioli | BCC1680 | Clinical | USA | ERS784912 | ERS4267800 | GCA_902831205 | 68 (Illumina) | 7.97 | 68.00 | 411,881 |
| Burkholderia gladioli | BCC1681 | Clinical | USA | ERS784927 | ERS4267801 | GCA_902831305 | 79 (Illumina) | 7.33 | 68.09 | 382,283 |
| Burkholderia gladioli | BCC1682 | Clinical | USA | ERS784944 | ERS4267802 | GCA_902829635 | 82 (Illumina) | 7.95 | 68.03 | 428,648 |
| Burkholderia gladioli | BCC1683 | Clinical | USA | ERS784962 | ERS4267803 | GCA_902831235 | 64 (Illumina) | 8.31 | 68.17 | 886,986 |
| Burkholderia gladioli | BCC1684 | Clinical | USA | ERS784975 | ERS4267804 | GCA_902831185 | 76 (Illumina) | 8.39 | 67.96 | 576,064 |
| Burkholderia gladioli | BCC1685 | Clinical | USA | ERS784983 | ERS4267805 | GCA_902829495 | 81 (Illumina) | 7.95 | 68.03 | 314,381 |
| Burkholderia gladioli | BCC1686 | Clinical | USA | ERS784816 | ERS4267806 | GCA_902831255 | 88 (Illumina) | 8.34 | 68.06 | 728,975 |
| Burkholderia gladioli | BCC1687 | Clinical | USA | ERS784833 | ERS4267807 | GCA_902831145 | 65 (Illumina) | 8.19 | 68.21 | 336,464 |
| Burkholderia gladioli | BCC1688 | Clinical | USA | ERS784848 | ERS4267808 | GCA_902831125 | 67 (Illumina) | 8.46 | 68.14 | 319,266 |
| Burkholderia gladioli | BCC1689 | Clinical | USA | ERS784865 | ERS4267809 | GCA_902831275 | 71 (Illumina) | 8.26 | 67.94 | 353,209 |
| Burkholderia gladioli | BCC1690 | Clinical | USA | ERS784881 | ERS4267810 | GCA_902831295 | 71 (Illumina) | 8.48 | 67.94 | 433,041 |
| Burkholderia gladioli | BCC1691 | Clinical | USA | ERS784898 | ERS4267811 | GCA_902831245 | 78 (Illumina) | 8.43 | 68.04 | 1,964,774 |
| Burkholderia gladioli | BCC1692 | Clinical | USA | ERS784914 | ERS4267812 | GCA_902831375 | 65 (Illumina) | 8.39 | 67.98 | 810,454 |
| Burkholderia gladioli | BCC1693 | Clinical | USA | ERS784929 | ERS4267813 | GCA_902831395 | 79 (Illumina) | 8.22 | 68.10 | 668,608 |
| Burkholderia gladioli | BCC1694 | Clinical | USA | ERS784946 | ERS4267814 | GCA_902831415 | 69 (Illumina) | 8.48 | 67.90 | 688,705 |
| Burkholderia gladioli | BCC1695 | Clinical | USA | ERS784964 | ERS4267815 | GCA_902831335 | 78 (Illumina) | 7.91 | 68.06 | 538,303 |
| Burkholderia gladioli | BCC1696 | Clinical | USA | ERS784976 | ERS4267816 | GCA_902831405 | 84 (Illumina) | 8.28 | 68.19 | 429,819 |
| Burkholderia gladioli | BCC1697 | Clinical | USA | ERS784984 | ERS4267817 | GCA_902831465 | 78 (Illumina) | 8.09 | 68.02 | 652,756 |
| Burkholderia gladioli | BCC1698 | Clinical | USA | ERS784818 | ERS4267818 | GCA_902831425 | 77 (Illumina) | 8.50 | 68.10 | 434,629 |
| Burkholderia gladioli | BCC1699 | Clinical | USA | ERS784834 | ERS4267819 | GCA_902831435 | 66 (Illumina) | 8.35 | 67.87 | 354,820 |
| Burkholderia gladioli | BCC1700 | Clinical | USA | ERS784851 | ERS4267820 | GCA_902831515 | 67 (Illumina) | 8.49 | 68.08 | 1,416,119 |
| Burkholderia gladioli | BCC1701 | Clinical | USA | ERS784867 | ERS4267821 | GCA_902829355 | 67 (Illumina) | 8.15 | 67.98 | 280,973 |
| Burkholderia gladioli | BCC1703 | Clinical | USA | ERS785056 | ERS4267822 | GCA_902831355 | 64 (Illumina) | 8.29 | 68.22 | 599,795 |
| Caballeronia sp. | BCC1704 | Clinical | USA | ERS784900 | ERS4267823 | GCA_902833455 | 87 (Illumina) | 5.98 | 63.70 | 1,316,445 |
| Burkholderia gladioli | BCC1705 | Clinical | USA | ERS784916 | ERS4267824 | GCA_902831345 | 79 (Illumina) | 8.15 | 68.17 | 551,683 |
| Caballeronia zhejiangensis | BCC1706 | Clinical | USA | ERS784931 | ERS4267825 | GCA_902833575 | 80 (Illumina) | 7.02 | 63.19 | 368,929 |
| Burkholderia gladioli | BCC1707 | Clinical | USA | ERS784948 | ERS4267826 | GCA_902831505 | 81 (Illumina) | 8.01 | 68.22 | 534,508 |
| Burkholderia gladioli | BCC1708 | Clinical | USA | ERS784966 | ERS4267827 | GCA_902831455 | 80 (Illumina) | 8.34 | 68.03 | 945,197 |
| Burkholderia gladioli | BCC1709 | Clinical | USA | ERS785035 | ERS4267828 | GCA_902831485 | 62 (Illumina) | 8.47 | 68.12 | 414,686 |
| Burkholderia gladioli | BCC1710 | Clinical | USA | ERS785039 | ERS4267829 | GCA_902831495 | 66 (Illumina) | 8.38 | 68.02 | 615,977 |
| Burkholderia gladioli | BCC1711 | Clinical | USA | ERS785006 | ERS4267830 | GCA_902831365 | 71 (Illumina) | 8.35 | 68.06 | 335,836 |
| Burkholderia gladioli | BCC1712 | Clinical | USA | ERS785025 | ERS4267831 | GCA_902831475 | 61 (Illumina) | 8.21 | 68.08 | 388,388 |
| Burkholderia gladioli | BCC1713 | Clinical | USA | ERS785074 | ERS4267832 | GCA_902831445 | 66 (Illumina) | 8.47 | 68.05 | 147,146 |
| Burkholderia gladioli | BCC1714 | Clinical | USA | ERS785080 | ERS4267833 | GCA_902831385 | 71 (Illumina) | 8.50 | 68.08 | 361,363 |
| Burkholderia gladioli | BCC1715 | Clinical | USA | ERS784977 | ERS4267834 | GCA_902831325 | 82 (Illumina) | 7.97 | 68.14 | 954,999 |
| Burkholderia gladioli | BCC1716 | Clinical | USA | ERS785052 | ERS4267835 | GCA_902831605 | 66 (Illumina) | 8.50 | 67.87 | 385,075 |
| Burkholderia gladioli | BCC1717 | Clinical | USA | ERS785014 | ERS4267836 | GCA_902831675 | 58 (Illumina) | 8.43 | 67.93 | 430,020 |
| Burkholderia gladioli | BCC1719 | Clinical | USA | ERS785042 | ERS4267837 | GCA_902831715 | 62 (Illumina) | 7.99 | 68.17 | 429,154 |
| Burkholderia gladioli | BCC1720 | Clinical | USA | ERS784985 | ERS4267838 | GCA_902831695 | 83 (Illumina) | 7.91 | 68.29 | 770,588 |
| Burkholderia gladioli | BCC1721 | Clinical | USA | ERS784820 | ERS4267839 | GCA_902831535 | 87 (Illumina) | 8.22 | 68.23 | 1,289,329 |
| Burkholderia gladioli | BCC1722 | Clinical | USA | ERS784836 | ERS4267840 | GCA_902831595 | 71 (Illumina) | 8.00 | 68.18 | 346,974 |
| Burkholderia gladioli | BCC1723 | Clinical | USA | ERS784853 | ERS4267841 | GCA_902831645 | 69 (Illumina) | 8.19 | 68.25 | 1,570,053 |
| Burkholderia gladioli | BCC1724 | Clinical | USA | ERS785060 | ERS4267842 | GCA_902831545 | 60 (Illumina) | 8.52 | 68.03 | 505,438 |
| Burkholderia gladioli | BCC1725 | Clinical | USA | ERS784869 | ERS4267843 | GCA_902831555 | 62 (Illumina) | 8.57 | 68.03 | 521,790 |
| Burkholderia gladioli | BCC1726 | Clinical | USA | ERS784885 | ERS4267844 | GCA_902831525 | 71 (Illumina) | 8.13 | 68.29 | 848,284 |
| Burkholderia gladioli | BCC1727 | Clinical | USA | ERS785068 | ERS4267845 | GCA_902829345 | 67 (Illumina) | 8.16 | 68.29 | 921,754 |
| Burkholderia gladioli | BCC1728 | Clinical | USA | ERS785003 | ERS4267846 | GCA_902831685 | 74 (Illumina) | 8.13 | 68.29 | 391,569 |
| Burkholderia gladioli | BCC1729 | Clinical | USA | ERS784902 | ERS4267847 | GCA_902831705 | 69 (Illumina) | 8.38 | 67.89 | 662,719 |
| Burkholderia gladioli | BCC1730 | Clinical | USA | ERS784917 | ERS4267848 | GCA_902829545 | 70 (Illumina) | 8.54 | 68.12 | 793,173 |
| Burkholderia gladioli | BCC1731 | Clinical | USA | ERS784997 | ERS4267849 | GCA_902831635 | 62 (Illumina) | 8.50 | 67.97 | 339,104 |
| Burkholderia gladioli | BCC1732 | Clinical | USA | ERS784933 | ERS4267850 | GCA_902829585 | 69 (Illumina) | 8.82 | 67.67 | 606,420 |
| Burkholderia gladioli | BCC1733 | Clinical | USA | ERS784951 | ERS4267851 | GCA_902831575 | 73 (Illumina) | 8.47 | 68.03 | 519,208 |
| Burkholderia gladioli | BCC1734 | Clinical | USA | ERS785079 | ERS4267852 | GCA_902831585 | 78 (Illumina) | 8.20 | 68.24 | 757,647 |
| Burkholderia gladioli | BCC1735 | Clinical | USA | ERS785008 | ERS4267853 | GCA_902831665 | 69 (Illumina) | 7.93 | 67.98 | 388,060 |
| Burkholderia gladioli | BCC1754 | Clinical | USA | ERS1371583 | ERS4267854 | GCA_902829365 | 64 (Illumina) | 8.38 | 68.20 | 416,499 |
| Burkholderia gladioli | BCC1755 | Clinical | USA | ERS1328941 | ERS4267855 | GCA_902831625 | 67 (Illumina) | 8.27 | 68.20 | 324,811 |
| Burkholderia gladioli | BCC1756 | Clinical | USA | ERS1328753 | ERS4267856 | GCA_902831655 | 75 (Illumina) | 7.75 | 68.11 | 264,066 |
| Burkholderia gladioli | BCC1757 | Clinical | USA | ERS1371584 | ERS4267857 | GCA_902831565 | 70 (Illumina) | 8.11 | 68.25 | 281,288 |
| Burkholderia gladioli | BCC1758 | Clinical | USA | ERS1371585 | ERS4267858 | GCA_902831615 | 77 (Illumina) | 8.42 | 68.16 | 347,044 |
| Burkholderia gladioli | BCC1759 | Clinical | USA | ERS1333276 | ERS4267859 | GCA_902831785 | 71 (Illumina) | 8.42 | 67.93 | 202,663 |
| Burkholderia gladioli | BCC1760 | Clinical | USA | ERS1371586 | ERS4267860 | GCA_902831885 | 74 (Illumina) | 8.15 | 68.21 | 351,231 |
| Burkholderia gladioli | BCC1761 | Clinical | USA | ERS1371587 | ERS4267861 | GCA_902831865 | 72 (Illumina) | 8.11 | 68.15 | 227,067 |
| Burkholderia gladioli | BCC1762 | Clinical | USA | ERS1328756 | ERS4267862 | GCA_902831745 | 78 (Illumina) | 8.38 | 67.86 | 232,045 |
| Burkholderia gladioli | BCC1763 | Clinical | USA | ERS1328942 | ERS4267863 | GCA_902831845 | 70 (Illumina) | 8.73 | 68.08 | 409,905 |
| Burkholderia gladioli | BCC1764 | Clinical | USA | ERS1371588 | ERS4267864 | GCA_902831775 | 76 (Illumina) | 7.95 | 68.19 | 273,951 |
| Burkholderia gladioli | BCC1765 | Clinical | USA | ERS1328883 | ERS4267865 | GCA_902831855 | 76 (Illumina) | 8.06 | 68.10 | 267,531 |
| Burkholderia gladioli | BCC1766 | Clinical | USA | ERS1371589 | ERS4267866 | GCA_902831735 | 77 (Illumina) | 8.28 | 67.89 | 275,070 |
| Burkholderia gladioli | BCC1767 | Clinical | USA | ERS1371590 | ERS4267867 | GCA_902831875 | 79 (Illumina) | 8.15 | 68.28 | 365,649 |
| Burkholderia gladioli | BCC1768 | Clinical | USA | ERS1371591 | ERS4267868 | GCA_902831905 | 71 (Illumina) | 8.26 | 68.11 | 226,248 |
| Burkholderia gladioli | BCC1769 | Clinical | USA | ERS1328884 | ERS4267869 | GCA_902831815 | 67 (Illumina) | 8.37 | 68.02 | 248,528 |
| Burkholderia gladioli | BCC1770 | Clinical | USA | ERS1328772 | ERS4267870 | GCA_902831755 | 61 (Illumina) | 8.42 | 67.98 | 261,993 |
| Burkholderia gladioli | BCC1771 | Clinical | USA | ERS1328943 | ERS4267871 | GCA_902831805 | 58 (Illumina) | 8.41 | 67.99 | 303,762 |
| Burkholderia gladioli | BCC1772 | Clinical | USA | ERS1328773 | ERS4267872 | GCA_902831895 | 58 (Illumina) | 8.44 | 68.03 | 340,707 |
| Burkholderia gladioli | BCC1773 | Clinical | USA | ERS1328885 | ERS4267873 | GCA_902829405 | 55 (Illumina) | 8.08 | 68.21 | 264,067 |
| Burkholderia gladioli | BCC1774 | Clinical | USA | ERS1371592 | ERS4267874 | GCA_902831835 | 70 (Illumina) | 7.89 | 68.10 | 264,045 |
| Burkholderia gladioli | BCC1775 | Clinical | USA | ERS1333277 | ERS4267875 | GCA_902831915 | 65 (Illumina) | 8.38 | 68.13 | 332,589 |
| Burkholderia gladioli | BCC1777 | Clinical | USA | ERS1328886 | ERS4267877 | GCA_902831795 | 67 (Illumina) | 7.93 | 68.09 | 275,756 |
| Burkholderia gladioli | BCC1778 | Clinical | USA | ERS1328776 | ERS4267878 | GCA_902831825 | 66 (Illumina) | 8.30 | 68.04 | 284,651 |
| Burkholderia gladioli | BCC1779 | Clinical | USA | ERS1328944 | ERS4267879 | GCA_902831765 | 65 (Illumina) | 8.27 | 68.02 | 190,616 |
| Burkholderia gladioli | BCC1780 | Clinical | USA | ERS1328777 | ERS4267880 | GCA_902831945 | 72 (Illumina) | 8.45 | 67.76 | 220,441 |
| Burkholderia gladioli | BCC1781 | Clinical | USA | ERS1328887 | ERS4267881 | GCA_902831975 | 69 (Illumina) | 7.99 | 68.16 | 208,905 |
| Burkholderia gladioli | BCC1782 | Clinical | USA | ERS1328778 | ERS4267882 | GCA_902829555 | 62 (Illumina) | 8.00 | 68.24 | 264,129 |
| Burkholderia gladioli | BCC1783 | Clinical | USA | ERS1336060 | ERS4267883 | GCA_902829505 | 61 (Illumina) | 8.39 | 67.96 | 224,024 |
| Burkholderia gladioli | BCC1784 | Clinical | USA | ERS1328888 | ERS4267884 | GCA_902831925 | 77 (Illumina) | 8.13 | 68.17 | 273,802 |
| Burkholderia gladioli | BCC1785 | Clinical | USA | ERS1328779 | ERS4267885 | GCA_902831985 | 75 (Illumina) | 7.99 | 68.26 | 243,147 |
| Burkholderia gladioli | BCC1786 | Clinical | USA | ERS1328945 | ERS4267886 | GCA_902831965 | 57 (Illumina) | 8.33 | 68.18 | 347,752 |
| Burkholderia gladioli | BCC1787 | Clinical | USA | ERS1371593 | ERS4267887 | GCA_902831955 | 96 (Illumina) | 8.25 | 68.27 | 371,296 |
| Burkholderia gladioli | BCC1788 | Clinical | USA | ERS1371594 | ERS4267888 | GCA_902831935 | 68 (Illumina) | 8.66 | 67.84 | 291,652 |
| Burkholderia gladioli | BCC1789 | Clinical | USA | ERS1328781 | ERS4267889 | GCA_902830195 | 61 (Illumina) | 8.33 | 68.05 | 274,262 |
| Burkholderia gladioli | BCC1790 | Clinical | USA | ERS1333278 | ERS4267890 | GCA_902831995 | 78 (Illumina) | 8.41 | 67.90 | 249,452 |
| Burkholderia gladioli | BCC1791 | Clinical | USA | ERS1328782 | ERS4267891 | GCA_902832115 | 68 (Illumina) | 8.15 | 68.14 | 334,966 |
| Burkholderia gladioli | BCC1792 | Clinical | USA | ERS1328890 | ERS4267892 | GCA_902832095 | 62 (Illumina) | 8.13 | 68.17 | 276,382 |
| Burkholderia gladioli | BCC1793 | Clinical | USA | ERS1328783 | ERS4267893 | GCA_902832155 | 73 (Illumina) | 8.14 | 68.24 | 280,591 |
| Burkholderia gladioli | BCC1794 | Clinical | USA | ERS1328946 | ERS4267894 | GCA_902832105 | 70 (Illumina) | 8.34 | 68.23 | 281,967 |
| Burkholderia gladioli | BCC1795 | Clinical | USA | ERS1328784 | ERS4267895 | GCA_902832035 | 77 (Illumina) | 7.82 | 68.17 | 275,050 |
| Burkholderia gladioli | BCC1796 | Clinical | USA | ERS1328891 | ERS4267896 | GCA_902832055 | 66 (Illumina) | 8.38 | 68.08 | 351,151 |
| Burkholderia gladioli | BCC1798 | Clinical | USA | ERS1371596 | ERS4267897 | GCA_902832005 | 75 (Illumina) | 8.25 | 68.07 | 230,356 |
| Burkholderia gladioli | BCC1799 | Clinical | USA | ERS1328786 | ERS4267898 | GCA_902832015 | 57 (Illumina) | 8.11 | 68.22 | 343,906 |
| Burkholderia gladioli | BCC1800 | Clinical | USA | ERS1328892 | ERS4267899 | GCA_902832165 | 84 (Illumina) | 7.92 | 68.29 | 257,500 |
| Burkholderia gladioli | BCC1801 | Clinical | USA | ERS1328787 | ERS4267900 | GCA_902832175 | 75 (Illumina) | 7.96 | 68.24 | 316,850 |
| Burkholderia gladioli | BCC1802 | Clinical | USA | ERS1328947 | ERS4267901 | GCA_902832185 | 56 (Illumina) | 8.45 | 68.11 | 270,804 |
| Burkholderia gladioli | BCC1803 | Clinical | USA | ERS1371597 | ERS4267902 | GCA_902832025 | 81 (Illumina) | 7.71 | 68.12 | 272,468 |
| Burkholderia gladioli | BCC1804 | Clinical | USA | ERS1371598 | ERS4267903 | GCA_902832195 | 71 (Illumina) | 8.45 | 68.00 | 250,984 |
| Burkholderia gladioli | BCC1805 | Clinical | USA | ERS1328789 | ERS4267904 | GCA_902832045 | 78 (Illumina) | 7.86 | 67.94 | 270,833 |
| Burkholderia gladioli | BCC1806 | Clinical | USA | ERS1333279 | ERS4267905 | GCA_902832075 | 63 (Illumina) | 8.48 | 68.05 | 273,269 |
| Burkholderia gladioli | BCC1807 | Clinical | USA | ERS1371599 | ERS4267906 | GCA_902832085 | 69 (Illumina) | 8.47 | 68.00 | 304,296 |
| Burkholderia gladioli | BCC1808 | Clinical | USA | ERS1371600 | ERS4267907 | GCA_902832145 | 77 (Illumina) | 7.97 | 68.22 | 293,939 |
| Burkholderia gladioli | BCC1809 | Clinical | USA | ERS1328791 | ERS4267908 | GCA_902832125 | 66 (Illumina) | 8.64 | 67.87 | 235,923 |
| Burkholderia gladioli | BCC1810 | Clinical | USA | ERS1328948 | ERS4267909 | GCA_902830185 | 64 (Illumina) | 8.28 | 68.22 | 310,714 |
| Burkholderia gladioli | BCC1811 | Clinical | USA | ERS1328792 | ERS4267910 | GCA_902832135 | 57 (Illumina) | 8.61 | 67.93 | 305,514 |
| Burkholderia gladioli | BCC1812 | Clinical | USA | ERS1328895 | ERS4267911 | GCA_902832065 | 73 (Illumina) | 8.23 | 67.94 | 301,688 |
| Burkholderia gladioli | BCC1813 | Clinical | USA | ERS1328793 | ERS4267912 | GCA_902832315 | 65 (Illumina) | 8.57 | 68.01 | 293,202 |
| Burkholderia gladioli | BCC1814 | Clinical | USA | ERS1336062 | ERS4267913 | GCA_902832285 | 62 (Illumina) | 8.21 | 68.14 | 206,859 |
| Burkholderia gladioli | BCC1815 | Clinical | USA | ERS1328794 | ERS4267914 | GCA_902832295 | 50 (Illumina) | 8.95 | 67.39 | 262,346 |
| Burkholderia gladioli | BCC1816 | Clinical | Canada | ERS1328896 | ERS4267915 | GCA_902832395 | 70 (Illumina) | 8.18 | 68.12 | 326,051 |
| Burkholderia gladioli | BCC1817 | Clinical | USA | ERS1328795 | ERS4267916 | GCA_902832305 | 69 (Illumina) | 8.12 | 68.21 | 242,936 |
| Burkholderia gladioli | BCC1818 | Clinical | USA | ERS1333280 | ERS4267917 | GCA_902832365 | 56 (Illumina) | 8.16 | 68.23 | 345,879 |
| Burkholderia gladioli | BCC1819 | Clinical | USA | ERS1328796 | ERS4267918 | GCA_902832325 | 61 (Illumina) | 8.42 | 67.90 | 331,109 |
| Burkholderia gladioli | BCC1820 | Clinical | USA | ERS1328897 | ERS4267919 | GCA_902832245 | 75 (Illumina) | 8.05 | 68.15 | 242,112 |
| Burkholderia gladioli | BCC1821 | Clinical | USA | ERS1328797 | ERS4267920 | GCA_902829485 | 76 (Illumina) | 8.13 | 68.08 | 263,017 |
| Burkholderia gladioli | BCC1822 | Clinical | USA | ERS1336063 | ERS4267921 | GCA_902832255 | 71 (Illumina) | 8.08 | 68.18 | 402,601 |
| Burkholderia gladioli | BCC1823 | Clinical | USA | ERS1328798 | ERS4267922 | GCA_902829525 | 69 (Illumina) | 8.22 | 68.31 | 336,871 |
| Paraburkholderia caribensis | BCC1824 | Clinical | USA | ERS1328898 | ERS4267923 | GCA_902833585 | 64 (Illumina) | 9.04 | 62.49 | 121,444 |
| Burkholderia gladioli | BCC1825 | Clinical | USA | ERS1328799 | ERS4267924 | GCA_902832355 | 70 (Illumina) | 8.21 | 67.96 | 211,609 |
| Burkholderia gladioli | BCC1826 | Clinical | USA | ERS1328949 | ERS4267925 | GCA_902832385 | 58 (Illumina) | 8.38 | 68.13 | 338,267 |
| Burkholderia gladioli | BCC1827 | Clinical | USA | ERS1328800 | ERS4267926 | GCA_902832345 | 58 (Illumina) | 8.71 | 67.88 | 269,894 |
| Burkholderia gladioli | BCC1828 | Clinical | Canada | ERS1328899 | ERS4267927 | GCA_902832375 | 78 (Illumina) | 8.24 | 68.04 | 268,035 |
| Burkholderia gladioli | BCC1829 | Clinical | USA | ERS1371601 | ERS4267928 | GCA_902832235 | 80 (Illumina) | 8.61 | 67.80 | 317,992 |
| Burkholderia gladioli | BCC1830 | Clinical | Canada | ERS1371602 | ERS4267929 | GCA_902832225 | 76 (Illumina) | 8.40 | 68.05 | 419,764 |
| Burkholderia gladioli | BCC1831 | Clinical | Canada | ERS1371603 | ERS4267930 | GCA_902832205 | 74 (Illumina) | 8.43 | 68.00 | 189,469 |
| Burkholderia gladioli | BCC1832 | Clinical | USA | ERS1371604 | ERS4267931 | GCA_902832215 | 73 (Illumina) | 8.16 | 68.08 | 208,866 |
| Burkholderia gladioli | BCC1833 | Clinical | USA | ERS1328803 | ERS4267932 | GCA_902832275 | 70 (Illumina) | 8.49 | 68.02 | 3902,44 |
| Burkholderia gladioli | BCC1834 | Clinical | USA | ERS1328950 | ERS4267933 | GCA_902832265 | 66 (Illumina) | 7.90 | 68.06 | 286,322 |
| Burkholderia gladioli | BCC1835 | Clinical | USA | ERS1371605 | ERS4267934 | GCA_902832335 | 73 (Illumina) | 8.41 | 67.96 | 264,845 |
| Burkholderia gladioli | BCC1836 | Clinical | USA | ERS1328901 | ERS4267935 | GCA_902832445 | 63 (Illumina) | 8.18 | 68.30 | 407,323 |
| Burkholderia gladioli | BCC1837 | Clinical | USA | ERS1371606 | ERS4267936 | GCA_902832455 | 77 (Illumina) | 8.14 | 67.99 | 206,652 |
| Burkholderia gladioli | BCC1838 | Clinical | USA | ERS1336064 | ERS4267937 | GCA_902830215 | 68 (Illumina) | 8.44 | 67.91 | 179,624 |
| Burkholderia gladioli | BCC1840 | Clinical | USA | ERS1328902 | ERS4267938 | GCA_902832405 | 71 (Illumina) | 8.15 | 68.14 | 209,087 |
| Burkholderia gladioli | BCC1841 | Clinical | USA | ERS1328807 | ERS4267939 | GCA_902832435 | 55 (Illumina) | 8.72 | 67.94 | 274,939 |
| Burkholderia gladioli | BCC1842 | Clinical | USA | ERS1328951 | ERS4267940 | GCA_902832415 | 76 (Illumina) | 8.17 | 68.27 | 344,808 |
| Burkholderia gladioli | BCC1843 | Clinical | USA | ERS1328808 | ERS4267941 | GCA_902832425 | 67 (Illumina) | 8.01 | 68.10 | 177,545 |
| Burkholderia gladioli | BCC1844 | Clinical | USA | ERS1328903 | ERS4267942 | GCA_902832555 | 71 (Illumina) | 8.20 | 67.98 | 219,436 |
| Burkholderia gladioli | BCC1845 | Clinical | USA | ERS1328809 | ERS4267943 | GCA_902832535 | 71 (Illumina) | 8.13 | 68.24 | 362,515 |
| Burkholderia gladioli | BCC1846 | Clinical | USA | ERS1371607 | ERS4267944 | GCA_902832475 | 68 (Illumina) | 8.41 | 68.00 | 320,953 |
| Burkholderia gladioli | BCC1847 | Clinical | USA | ERS1371608 | ERS4267945 | GCA_902830245 | 65 (Illumina) | 8.41 | 68.17 | 287,220 |
| Burkholderia gladioli | BCC1848 | Clinical | USA | ERS1328904 | ERS4267946 | GCA_902832525 | 69 (Illumina) | 8.14 | 68.04 | 301,164 |
| Burkholderia gladioli | BCC1849 | Clinical | USA | ERS1371609 | ERS4267947 | GCA_902832485 | 94 (Illumina) | 8.22 | 68.29 | 368,142 |
| Burkholderia gladioli | BCC1850 | Clinical | USA | ERS1371610 | ERS4267948 | GCA_902832585 | 81 (Illumina) | 8.53 | 68.02 | 275,270 |
| Burkholderia gladioli | BCC1851 | Clinical | USA | ERS1371611 | ERS4267949 | GCA_902832495 | 65 (Illumina) | 8.37 | 68.20 | 304,291 |
| Burkholderia gladioli | BCC1852 | Clinical | USA | ERS1371612 | ERS4267950 | GCA_902832515 | 72 (Illumina) | 8.42 | 68.05 | 333,375 |
| Burkholderia gladioli | BCC1853 | Clinical | USA | ERS1328813 | ERS4267951 | GCA_902832545 | 66 (Illumina) | 8.22 | 68.20 | 326,166 |
| Burkholderia gladioli | BCC1854 | Clinical | USA | ERS1371613 | ERS4267952 | GCA_902832465 | 69 (Illumina) | 8.90 | 67.81 | 274,690 |
| Burkholderia gladioli | BCC1855 | Clinical | USA | ERS1371614 | ERS4267953 | GCA_902832505 | 64 (Illumina) | 8.32 | 68.01 | 186,469 |
| Burkholderia gladioli | BCC1856 | Clinical | USA | ERS1328906 | ERS4267954 | GCA_902832565 | 67 (Illumina) | 8.05 | 68.29 | 286,277 |
| Burkholderia gladioli | BCC1857 | Clinical | USA | ERS1371615 | ERS4267955 | GCA_902832595 | 68 (Illumina) | 8.23 | 68.25 | 280,379 |
| Burkholderia gladioli | BCC1858 | Clinical | USA | ERS1371616 | ERS4267956 | GCA_902832575 | 54 (Illumina) | 8.19 | 68.22 | 307,394 |
| Burkholderia gladioli | BCC1859 | Clinical | USA | ERS1371617 | ERS4267957 | GCA_902832615 | 95 (Illumina) | 8.26 | 68.20 | 334,836 |
| Burkholderia gladioli | BCC1860 | Clinical | USA | ERS1371618 | ERS4267958 | GCA_902832655 | 88 (Illumina) | 8.63 | 68.00 | 347,760 |
| Burkholderia gladioli | BCC1861 | Clinical | USA | ERS1371619 | ERS4267959 | GCA_902829615 | 79 (Illumina) | 8.63 | 67.75 | 216,674 |
| Burkholderia gladioli | BCC1862 | Clinical | USA | ERS1371620 | ERS4267960 | GCA_902832685 | 89 (Illumina) | 8.38 | 68.14 | 294,407 |
| Burkholderia gladioli | BCC1863 | Clinical | USA | ERS1371621 | ERS4267961 | GCA_902832605 | 74 (Illumina) | 8.27 | 68.23 | 294,023 |
| Burkholderia gladioli | BCC1864 | Clinical | USA | ERS1328908 | ERS4267962 | GCA_902830235 | 53 (Illumina) | 8.50 | 67.90 | 212,901 |
| Burkholderia gladioli | BCC1865 | Clinical | USA | ERS1328819 | ERS4267963 | GCA_902832725 | 72 (Illumina) | 8.23 | 68.15 | 233,312 |
| Burkholderia gladioli | BCC1866 | Clinical | USA | ERS1371622 | ERS4267964 | GCA_902832695 | 71 (Illumina) | 8.46 | 67.99 | 287,257 |
| Burkholderia gladioli | BCC1867 | Clinical | USA | ERS1371623 | ERS4267965 | GCA_902832675 | 72 (Illumina) | 8.40 | 67.96 | 178,703 |
| Burkholderia gladioli | BCC1868 | Clinical | USA | ERS1371624 | ERS4267966 | GCA_902832715 | 62 (Illumina) | 7.93 | 68.19 | 393,583 |
| Burkholderia gladioli | BCC1869 | Clinical | USA | ERS1328821 | ERS4267967 | GCA_902832665 | 64 (Illumina) | 8.60 | 67.92 | 311,968 |
| Burkholderia gladioli | BCC1870 | Clinical | USA | ERS1371625 | ERS4267968 | GCA_902832645 | 86 (Illumina) | 8.35 | 67.95 | 186,380 |
| Burkholderia gladioli | BCC1871 | Clinical | USA | ERS1371626 | ERS4267969 | GCA_902832705 | 95 (Illumina) | 8.06 | 68.11 | 284,538 |
| Burkholderia gladioli | BCC1872 | Clinical | USA | ERS1371627 | ERS4267970 | GCA_902832745 | 74 (Illumina) | 8.43 | 68.15 | 286,407 |
| Burkholderia gladioli | BCC1880 | ERS1371628 | ERS4267971 | GCA_902832635 | 73 (Illumina) | 8.23 | 68.04 | 621,809 | ||
| Burkholderia gladioli | BCC1881 | ERS1371629 | ERS4267972 | GCA_902832735 | 71 (Illumina) | 8.39 | 68.20 | 350,910 | ||
| Burkholderia gladioli | BCC1882 | ERS1371630 | ERS4267973 | GCA_902832625 | 58 (Illumina) | 8.39 | 68.20 | 350,910 | ||
| Burkholderia gladioli | BCC1883 | ERS1371631 | ERS4267974 | GCA_902832785 | 71 (Illumina) | 8.13 | 68.31 | 603,194 | ||
| Burkholderia glumae | LMG 2196 | Environmental, Oryza sativa | Japan | ERS784969 | ERS4267975 | GCA_902832765 | 92 (Illumina) | 6.66 | 68.35 | 162,494 |
| Burkholderia lata | BCC0174 | Environmental, soil | Trinidad and Tobago | ERS785040 | ERS4267976 | GCA_902832775 | 63 (Illumina) | 8.48 | 66.57 | 1,023,694 |
| Burkholderia sp. | BCC0217 | Clinical, cystic fibrosis patient | USA | ERS784822 | ERS4267977 | GCA_902833005 | 74 (Illumina) | 9.44 | 65.63 | 453,341 |
| Burkholderia latens | BCC1625 | Clinical, cystic fibrosis patient | Italy | ERS784839 | ERS4267978 | GCA_902832795 | 80 (Illumina) | 7.08 | 66.83 | 469,972 |
| Burkholderia latens | BCC1626 | Clinical, cystic fibrosis patient | UK | ERS784855 | ERS4267979 | GCA_902832755 | 78 (Illumina) | 7.25 | 66.69 | 638,063 |
| Burkholderia metallica | BCC0095 | Clinical, sputum | Canada | ERS785019 | ERS4267980 | GCA_902832815 | 67 (Illumina) | 7.75 | 66.76 | 474,034 |
| Burkholderia metallica | BCC0483 | Clinical | ERS784872 | ERS4267981 | GCA_902832805 | 75 (Illumina) | 7.55 | 67.06 | 469,713 | |
| Burkholderia metallica | LMG 24068 | Clinical, cystic fibrosis patient | USA | ERS784887 | ERS4267982 | GCA_902832845 | 75 (Illumina) | 7.55 | 67.06 | 476,026 |
| Burkholderia multivorans | BCC0005 | Clinical, sputum | Canada | ERS785011 | ERS4267983 | GCA_902832835 | 87 (Illumina) | 6.39 | 67.37 | 1,402,971 |
| Burkholderia multivorans | BCC0008 | Clinical, sputum | UK | ERS784904 | ERS4267984 | GCA_902832825 | 88 (Illumina) | 6.63 | 67.18 | 793,813 |
| Burkholderia multivorans | BCC0010 | Clinical, non-cystic fibrosis patient brain abscess | UK | ERS784919 | ERS4267985 | GCA_902833875 | 92 (Illumina) | 6.27 | 67.24 | 486,046 |
| Burkholderia multivorans | BCC0031 | Clinical, sputum | Canada | ERS785002 | ERS4267986 | GCA_902833805 | 89 (Illumina) | 6.53 | 67.07 | 561,216 |
| Burkholderia multivorans | BCC0037 | Clinical, chronic granulomatous disease, lung | USA | ERS785053 | ERS4267987 | GCA_902833915 | 93 (Illumina) | 6.56 | 67.16 | 303,874 |
| Burkholderia multivorans | BCC0050 | Environmental, soil | UK | ERS785036 | ERS4267988 | GCA_902833885 | 78 (Illumina) | 6.97 | 66.86 | 531,523 |
| Burkholderia multivorans | BCC0059 | Clinical, sputum | Canada | ERS785022 | ERS4267989 | GCA_902833835 | 74 (Illumina) | 6.76 | 67.09 | 384,200 |
| Burkholderia multivorans | BCC0067 | Clinical, sputum | Canada | ERS784935 | ERS4267990 | GCA_902833855 | 96 (Illumina) | 6.12 | 67.36 | 349,267 |
| Burkholderia multivorans | BCC0089 | Clinical, sputum | Canada | ERS785033 | ERS4267991 | GCA_902833895 | 82 (Illumina) | 6.39 | 67.21 | 595,797 |
| Burkholderia multivorans | BCC0093 | Clinical, respiratory sample | Canada | ERS785061 | ERS4267992 | GCA_902832975 | 96 (Illumina) | 6.30 | 67.22 | 342,629 |
| Burkholderia multivorans | BCC0099 | Clinical, respiratory sample | Canada | ERS785069 | ERS4267993 | GCA_902832855 | 94 (Illumina) | 6.93 | 66.81 | 276,171 |
| Burkholderia multivorans | BCC0102 | Clinical, respiratory sample | Canada | ERS784954 | ERS4267994 | GCA_902833015 | 80 (Illumina) | 6.66 | 67.18 | 459,105 |
| Burkholderia multivorans | BCC0115 | Clinical, sputum | USA | ERS784971 | ERS4267995 | GCA_902830305 | 102 (Illumina) | 6.22 | 67.36 | 413,909 |
| Burkholderia multivorans | BCC0149 | Clinical, cystic fibrosis patient | USA | ERS785050 | ERS4267996 | GCA_902832965 | 86 (Illumina) | 6.35 | 67.17 | 436,404 |
| Burkholderia multivorans | BCC0175 | Clinical, chronic granulomatous disease, blood | USA | ERS785024 | ERS4267997 | GCA_902832875 | 77 (Illumina) | 6.71 | 66.72 | 328,297 |
| Burkholderia multivorans | BCC0244 | Clinical, sputum | Canada | ERS785004 | ERS4267998 | GCA_902832915 | 93 (Illumina) | 6.57 | 67.18 | 435,992 |
| Burkholderia oklahomensis | LMG 23618 | Environmental | ERS784979 | ERS4267999 | GCA_902829515 | 88 (Illumina) | 7.09 | 67.11 | 364,599 | |
| Burkholderia plantarii | LMG 9035 | Environmental, Oryza sativa cv. Koshihikari, seedling with blight and chlorosis | Japan | ERS784824 | ERS4268000 | GCA_902832905 | 79 (Illumina) | 8.04 | 68.61 | 305,422 |
| Burkholderia pyrrocinia | BCC0171 | Environmental, cornfield soil | USA | ERS785059 | ERS4268001 | GCA_902832995 | 66 (Illumina) | 8.16 | 66.18 | 325,972 |
| Burkholderia pyrrocinia | LMG 14191 | Environmental, soil | ERS785083 | ERS4268002 | GCA_902832895 | 75 (Illumina) | 7.86 | 66.51 | 520,567 | |
| Burkholderia pyrrocinia | BCC0986 | Environmental | ERS784857 | ERS4268003 | GCA_902829605 | 74 (Illumina) | 7.60 | 66.70 | 556,419 | |
| Burkholderia pyrrocinia | BCC1346 | Environmental | ERS784841 | ERS4268004 | GCA_902832935 | 70 (Illumina) | 8.34 | 65.94 | 282,972 | |
| Burkholderia pyrrocinia | BCC1364 | Clinical | ERS784874 | ERS4268005 | GCA_902832945 | 75 (Illumina) | 7.62 | 66.57 | 587,939 | |
| Burkholderia seminalis | LMG 24067 | Clinical, cystic fibrosis patient | USA | ERS784889 | ERS4268006 | GCA_902832885 | 70 (Illumina) | 7.97 | 67.09 | 523,234 |
| Burkholderia seminalis | BCC1628 | Environmental, Oryza sativa seed | Philippines | ERS784906 | ERS4268007 | GCA_902833035 | 60 (Illumina) | 8.24 | 66.71 | 331,981 |
| Burkholderia sp. | BCC1642 | Environmental | ERS1328853 | ERS4268008 | GCA_902829715 | 78 (Illumina) | 9.22 | 62.08 | 159,099 | |
| Burkholderia stabilis | BCC0186 | Clinical, cystic fibrosis patient | Belgium | ERS785081 | ERS4268009 | GCA_902833135 | 92 (Illumina) | 7.15 | 66.55 | 267,680 |
| Burkholderia stabilis | BCC0237 | Environmental, albuterol inhaler | USA | ERS785013 | ERS4268010 | GCA_902833115 | 58 (Illumina) | 8.52 | 66.46 | 323,418 |
| Burkholderia stabilis | BCC0285 | Clinical, infected tissue of tibial fracture | ERS784949 | ERS4268011 | GCA_902833105 | 80 (Illumina) | 8.75 | 66.23 | 274,531 | |
| Burkholderia stabilis | BCC1308 | Environmental/industrial | ERS784921 | ERS4268012 | GCA_902833185 | 61 (Illumina) | 8.25 | 66.51 | 685,734 | |
| Burkholderia stagnalis | LMG 28156 | Environmental, soil | Australia | ERS1371647 | ERS4268013 | GCA_902830275 | 66 (Illumina) | 8.03 | 67.24 | 262,379 |
| Burkholderia territorii | LMG 28158 | Environmental, water | Australia | ERS1328858 | ERS4268014 | GCA_902833055 | 92 (Illumina) | 6.98 | 66.66 | 277,911 |
| Burkholderia ubonensis | LMG 20358 | Environmental, surface soil | Thailand | ERS784956 | ERS4268015 | GCA_902833085 | 66 (Illumina) | 7.77 | 67.30 | 623,290 |
| Burkholderia vietnamiensis | BCC0027 | Clinical, cystic fibrosis patient | USA | ERS785078 | ERS4268016 | GCA_902833235 | 85 (Illumina) | 6.87 | 66.94 | 283,796 |
| Burkholderia vietnamiensis | BCC0028 | Clinical, cystic fibrosis patient | Sweden | ERS785077 | ERS4268017 | GCA_902833215 | 94 (Illumina) | 6.67 | 67.25 | 195,580 |
| Burkholderia vietnamiensis | BCC0029 | Clinical, chronic granulomatous disease, lung abscess | Canada | ERS784991 | ERS4268018 | GCA_902829455 | 95 (Illumina) | 6.98 | 66.73 | 204,529 |
| Burkholderia vietnamiensis | LMG 10929 | Environmental, Oryza sativa rhizosphere soil | Vietnam | ERS785016 | ERS4268019 | GCA_902830295 | 75 (Illumina) | 6.88 | 66.90 | 308,470 |
| Burkholderia vietnamiensis | BCC0040 | Clinical, non-cystic fibrosis patient | ERS784999 | ERS4268020 | GCA_902833385 | 89 (Illumina) | 6.26 | 66.68 | 397,866 | |
| Burkholderia vietnamiensis | BCC0042 | Environmental, hospital | ERS784972 | ERS4268021 | GCA_902833285 | 105 (Illumina) | 6.29 | 66.65 | 667,227 | |
| Burkholderia vietnamiensis | BCC0046 | Clinical, non-cystic fibrosis patient | ERS785044 | ERS4268022 | GCA_902833275 | 81 (Illumina) | 6.77 | 66.97 | 511,799 | |
| Burkholderia vietnamiensis | BCC0104 | Clinical, lymph node | USA | ERS785007 | ERS4268023 | GCA_902833335 | 74 (Illumina) | 7.06 | 66.85 | 228,863 |
| Burkholderia vietnamiensis | BCC0124 | Clinical, sputum | USA | ERS785030 | ERS4268024 | GCA_902833355 | 73 (Illumina) | 7.11 | 66.93 | 309,681 |
| Burkholderia vietnamiensis | BCC0126 | Clinical, sputum | USA | ERS1328823 | ERS4268025 | GCA_902833375 | 72 (Illumina) | 6.81 | 67.10 | 186,133 |
| Burkholderia vietnamiensis | BCC0128 | Clinical, sputum | Canada | ERS784980 | ERS4268026 | GCA_902833415 | 96 (Illumina) | 6.63 | 67.17 | 400,883 |
| Burkholderia vietnamiensis | BCC0129 | Clinical | USA | ERS1328846 | ERS4268027 | GCA_902833445 | 79 (Illumina) | 6.98 | 66.46 | 186,188 |
| Burkholderia vietnamiensis | BCC0136 | Clinical, throat | Canada | ERS785029 | ERS4268028 | GCA_902833435 | 69 (Illumina) | 7.29 | 66.68 | 225,055 |
| Burkholderia vietnamiensis | BCC0142 | Clinical | USA | ERS784988 | ERS4268029 | GCA_902833265 | 86 (Illumina) | 7.21 | 66.73 | 246,894 |
| Burkholderia vietnamiensis | BCC0151 | Clinical | USA | ERS784826 | ERS4268030 | GCA_902833395 | 95 (Illumina) | 6.08 | 67.33 | 168,450 |
| Burkholderia vietnamiensis | BCC0157 | Clinical, sputum | USA | ERS1371645 | ERS4268031 | GCA_902833295 | 102 (Illumina) | 6.35 | 67.18 | 137,809 |
| Burkholderia vietnamiensis | BCC0185 | Clinical, neck abscess | Chile | ERS1336070 | ERS4268032 | GCA_902833315 | 77 (Illumina) | 7.12 | 66.80 | 202,960 |
| Burkholderia vietnamiensis | BCC0190 | Clinical | USA | ERS785015 | ERS4268033 | GCA_902830265 | 84 (Illumina) | 6.16 | 66.76 | 523,281 |
| Burkholderia vietnamiensis | BCC0194 | Environmental, soil | ERS785075 | ERS4268034 | GCA_902833365 | 75 (Illumina) | 7.06 | 66.93 | 158,113 | |
| Burkholderia vietnamiensis | BCC0324 | Clinical, non-cystic fibrosis patient | ERS784843 | ERS4268035 | GCA_902833345 | 92 (Illumina) | 6.21 | 66.62 | 311,192 | |
| Burkholderia vietnamiensis | BCC0587 | Environmental, soil | ERS1328927 | ERS4268037 | GCA_902833305 | 71 (Illumina) | 7.39 | 66.79 | 187,227 | |
| Burkholderia vietnamiensis | BCC1161 | Clinical | ERS785038 | ERS4268038 | GCA_902833255 | 78 (Illumina) | 6.62 | 67.33 | 306,697 | |
| Burkholderia vietnamiensis | BCC1162 | Clinical | ERS784859 | ERS4268039 | GCA_902833325 | 85 (Illumina) | 6.62 | 67.33 | 308,591 | |
| Burkholderia vietnamiensis | BCC1163 | Clinical | ERS784876 | ERS4268040 | GCA_902833545 | 81 (Illumina) | 6.62 | 67.33 | 314,533 | |
| Burkholderia vietnamiensis | BCC1170 | Clinical | ERS785023 | ERS4268041 | GCA_902833495 | 79 (Illumina) | 6.91 | 66.39 | 157,040 | |
| Burkholderia vietnamiensis | BCC1172 | Environmental | ERS785031 | ERS4268042 | GCA_902833625 | 71 (Illumina) | 7.39 | 66.30 | 161,339 | |
| Burkholderia vietnamiensis | BCC1176 | Clinical | ERS785046 | ERS4268043 | GCA_902833605 | 76 (Illumina) | 7.09 | 66.83 | 457,880 | |
| Burkholderia vietnamiensis | BCC1186 | Clinical | ERS784891 | ERS4268044 | GCA_902833535 | 72 (Illumina) | 7.08 | 66.45 | 138,269 | |
| Burkholderia vietnamiensis | BCC1193 | Clinical | ERS785058 | ERS4268045 | GCA_902833555 | 72 (Illumina) | 7.25 | 66.29 | 160,650 | |
| Burkholderia vietnamiensis | BCC1301 | Environmental/industrial | ERS785065 | ERS4268046 | GCA_902833615 | 80 (Illumina) | 7.05 | 66.78 | 246,898 | |
| Burkholderia vietnamiensis | BCC1304 | Environmental/industrial | ERS784990 | ERS4268047 | GCA_902833475 | 71 (Illumina) | 8.09 | 66.11 | 246,358 | |
| Burkholderia vietnamiensis | BCC1309 | Environmental/industrial | ERS785063 | ERS4268048 | GCA_902833565 | 82 (Illumina) | 7.08 | 66.91 | 276,656 | |
| Burkholderia vietnamiensis | BCC1409 | Environmental/industrial | ERS785012 | ERS4268049 | GCA_902833595 | 72 (Illumina) | 7.22 | 66.67 | 308,880 | |
| Burkholderia vietnamiensis | BCC1412 | Environmental/industrial | ERS785020 | ERS4268050 | GCA_902833465 | 71 (Illumina) | 7.34 | 66.61 | 178,163 | |
| Burkholderia cepacia | BCC1633 | Environmental | Malaysia | ERS1328923 | ERS4268051 | GCA_902830795 | 81 (Illumina) | 8.84 | 66.29 | 275,215 |
| Caballeronia glathei | BCC0772 | Environmental, lateritic soil | Germany | ERS784993 | ERS4268052 | GCA_902833485 | 75 (Illumina) | 8.74 | 64.38 | 262,729 |
| Paraburkholderia bryophila | BCC1875 | Environmental, isolated from Sphagnum rubellum moss | Germany | ERS1328963 | ERS4268053 | GCA_902833525 | 71 (Illumina) | 8.09 | 62.91 | 200,899 |
| Paraburkholderia sp. | BCC1876 | Environmental, isolated from Aulacomnium palustre moss | Germany | ERS1328856 | ERS4268054 | GCA_902833695 | 68 (Illumina) | 8.50 | 62.75 | 785,957 |
| Paraburkholderia caledonica | LMG 19076 | Environmental, soil rhizosphere | UK | ERS784802 | ERS4268055 | GCA_902833635 | 99 (Illumina) | 7.31 | 61.96 | 449,131 |
| Paraburkholderia caribensis | LMG 18531 | Environmental, soil, vertisol | ERS784810 | ERS4268056 | GCA_902833515 | 66 (Illumina) | 8.97 | 62.60 | 301,636 | |
| Paraburkholderia fungorum | LMG 16225 | Environmental, Phanerochaete chrysosporium fungus | France | ERS784892 | ERS4268057 | GCA_902833645 | 67 (Illumina) | 8.93 | 61.85 | 451,759 |
| Paraburkholderia hospita | LMG 20598 | Environmental, agricultural soil | Belgium | ERS784978 | ERS4268058 | GCA_902833685 | 56 (Illumina) | 11.25 | 61.88 | 202,916 |
| Paraburkholderia kururiensis | LMG 19447 | Environmental, aquifer | Japan | ERS784986 | ERS4268059 | GCA_902833705 | 84 (Illumina) | 7.54 | 64.83 | 257,717 |
| Burkholderia vietnamiensis | BCC1878 | Environmental, isolated from Aulacomnium palustre moss | Germany | ERS1328857 | ERS4268060 | GCA_902829795 | 91 (Illumina) | 7.03 | 66.73 | 129,098 |
| Paraburkholderia mimosarum | LMG 23256 | Environmental, Mimosa pigra root nodule | China | ERS785026 | ERS4268061 | GCA_902833725 | 60 (Illumina) | 8.60 | 63.84 | 198,630 |
| Paraburkholderia phenazinium | BCC1873 | Environmental | ERS1328926 | ERS4268062 | GCA_902829745 | 70 (Illumina) | 8.96 | 62.26 | 287,222 | |
| Paraburkholderia phenazinium | BCC1874 | ERS1328855 | ERS4268063 | GCA_902833675 | 71 (Illumina) | 9.00 | 62.28 | 309,756 | ||
| Paraburkholderia phenoliruptrix | LMG 22037 | Environmental, chemostat | ERS1371595 | ERS4268064 | GCA_902833655 | 82 (Illumina) | 7.36 | 63.48 | 174,050 | |
| Paraburkholderia phymatum | LMG 21445 | Environmental, Machaerium lunatum root nodule | French Guiana | ERS784987 | ERS4268065 | GCA_902833665 | 72 (Illumina) | 8.60 | 62.32 | 334,756 |
| Paraburkholderia sacchari | LMG 19450 | Environmental, sugarcane plantation soil | Brazil | ERS785005 | ERS4268066 | GCA_902833715 | 81 (Illumina) | 7.27 | 64.04 | 428,857 |
| Paraburkholderia terricola | LMG 20594 | Environmental, agricultural soil | Belgium | ERS785021 | ERS4268067 | GCA_902833815 | 67 (Illumina) | 7.34 | 63.65 | 179,029 |
| Paraburkholderia tropica | LMG 22274 | Environmental, sugarcane roots | Brazil | ERS784938 | ERS4268068 | GCA_902833865 | 60 (Illumina) | 8.60 | 64.77 | 492,153 |
| Paraburkholderia tropica | BCC1637 | Environmental | Malaysia | ERS1328924 | ERS4268069 | GCA_902833825 | 80 (Illumina) | 8.35 | 64.80 | 300,108 |
| Paraburkholderia tuberum | LMG 21444 | Environmental, Aspalathus carnosa root nodule | South Africa | ERS785070 | ERS4268070 | GCA_902833905 | 64 (Illumina) | 8.79 | 63.06 | 137,501 |
| Robbsia andropogonis | LMG 2129 | Environmental, Sorghum bicolor | USA | ERS784901 | ERS4268071 | GCA_902833845 | 84 (Illumina) | 6.33 | 58.87 | 340,675 |
| Trinickia caryophylli | LMG 2155 | Environmental, Dianthus caryophyllus | USA | ERS784825 | ERS4268072 | GCA_902833925 | 104 (Illumina) | 6.56 | 64.73 | 645,930 |
| Burkholderia stagnalis | BCC1350 | Environmental | ERS4268073 | GCA_902833205 | 150 (PacBio) | 8.45 | 67.06 | 3,279,208 | ||
| Burkholderia gladioli | BCC0238 | Clinical, sputum | USA | ERS4268074 | GCA_902829535 | 150 (PacBio) | 8.64 | 67.94 | 4,046,521 | |
| Burkholderia vietnamiensis | BCC0268 | Environmental, soil | New Zealand | ERS4268075 | GCA_902833425 | 150 (PacBio) | 6.81 | 67.10 | 2,221,444 | |
| Burkholderia vietnamiensis | BCC1408 | Environmental/industrial, diesel contaminant | UK | ERS4268076 | GCA_902833505 | 150 (PacBio) | 7.89 | 66.39 | 1,872,953 |
The genome collection contains 24 named Burkholderia species and 17 genomes that possessed an ANI value of <95% compared to sequenced type strains. Within the Burkholderia cepacia complex, the first representative genome of Burkholderia arboris has been deposited, as well as genomes for type strains of Burkholderia anthina, Burkholderia metallica, Burkholderia seminalis, Burkholderia territorii, and Burkholderia ubonensis. The collection considerably increases the number of curated Burkholderia genomes, specifically for Burkholderia gladioli (207 genomes), Burkholderia ambifaria (60 genomes), and Burkholderia vietnamiensis (35 genomes).
The Paraburkholderia genomes cover 14 named species and 1 unnamed species based on ANI analysis. The type strains of Paraburkholderia caledonica and Paraburkholderia tuberum were sequenced, and the type strains of 11 species were resequenced. Additional genome assemblies were deposited for Paraburkholderia megapolitana and Paraburkholderia phenazinium. Finally, three Caballeronia genomes were identified in the collection, i.e., Caballeronia glathei, Caballeronia zhejiangensis, and an uncharacterized Caballeronia species. The type strains of Robbsia andropogonis and Trinickia caryophylli were also resequenced.
Multiple novel metabolites have been identified, and their biosynthetic pathways have been elucidated, including the antimycobacterial compound gladiolin (9) and the lipopeptidiolide antibiotic icosalides (15) in B. gladioli. The discovery and biosynthesis of enacyloxin lla, an antibiotic with potent activity against Acinetobacter baumannii (16–18), and the antioomycete metabolite cepacin (19) have been studied in B. ambifaria. Further analysis of this Burkholderiaceae genome resource is under way to identify additional biologically active specialized metabolites and their potential applications.
Data availability.
The genome sequences in this announcement have been deposited in the European Nucleotide Archive (ENA) under the project accession number PRJEB35318. The BioSample accession numbers for the 454 genome assemblies are ERS4267623 to ERS4268076. The Illumina paired-end read data associated with these genome assemblies have been deposited under the project accession number PRJEB9765, with the exception of Burkholderia diffusa LMG 29043, which is available under project accession number PRJEB35318.
ACKNOWLEDGMENTS
This study was supported by grants from the Biotechnology and Biological Sciences Research Council (BBSRC) (grants BB/L021692/1 and BB/S007652/1). All analyses were performed using the Medical Research Council (MRC) Cloud Infrastructure for Microbial Bioinformatics (grant MR/L015080/1). A.J.M. acknowledges support from the BBSRC South West Doctoral Training Partnership (grant BB/M009122/1).
We are grateful for the assistance of the Wellcome Sanger Institute core sequencing and informatics groups. We thank John LiPuma (University of Michigan) and collaborators in the International Burkholderia cepacia Working Group (https://ibcwg.org) for providing bacterial strains used in this study. All bacterial strains in this study are curated in the Burkholderia culture collection at Cardiff University.
REFERENCES
- 1.Kunakom S, Eustáquio AS. 2019. Burkholderia as a source of natural products. J Nat Prod 82:2018–2037. doi: 10.1021/acs.jnatprod.8b01068. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 2.Zerikly M, Challis GL. 2009. Strategies for the discovery of new natural products by genome mining. Chembiochem 10:625–633. doi: 10.1002/cbic.200800389. [DOI] [PubMed] [Google Scholar]
- 3.Krueger F. 2016. Trim Galore! https://www.bioinformatics.babraham.ac.uk/projects/trim_galore.
- 4.Andrews S. 2009. FastQC: a quality control tool for high throughput sequence data. https://www.bioinformatics.babraham.ac.uk/projects/fastqc.
- 5.Magoč T, Salzberg SL. 2011. FLASH: fast length adjustment of short reads to improve genome assemblies. Bioinformatics 27:2957–2963. doi: 10.1093/bioinformatics/btr507. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 6.Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS, Lesin VM, Nikolenko SI, Pham S, Prjibelski AD, Pyshkin AV, Sirotkin AV, Vyahhi N, Tesler G, Alekseyev MA, Pevzner PA. 2012. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 19:455–477. doi: 10.1089/cmb.2012.0021. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 7.Walker BJ, Abeel T, Shea T, Priest M, Abouelliel A, Sakthikumar S, Cuomo CA, Zeng Q, Wortman J, Young SK, Earl AM. 2014. Pilon: an integrated tool for comprehensive microbial variant detection and genome assembly improvement. PLoS One 9:e112963. doi: 10.1371/journal.pone.0112963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 8.Wood DE, Salzberg SL. 2014. Kraken: ultrafast metagenomic sequence classification using exact alignments. Genome Biol 15:R46. doi: 10.1186/gb-2014-15-3-r46. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 9.Song L, Jenner M, Masschelein J, Jones C, Bull MJ, Harris SR, Hartkoorn RC, Vocat A, Romero-Canelon I, Coupland P, Webster G, Dunn M, Weiser R, Paisey C, Cole ST, Parkhill J, Mahenthiralingam E, Challis GL. 2017. Discovery and biosynthesis of gladiolin: a Burkholderia gladioli antibiotic with promising activity against Mycobacterium tuberculosis. J Am Chem Soc 139:7974–7981. doi: 10.1021/jacs.7b03382. [DOI] [PubMed] [Google Scholar]
- 10.Gurevich A, Saveliev V, Vyahhi N, Tesler G. 2013. QUAST: quality assessment tool for genome assemblies. Bioinformatics 29:1072–1075. doi: 10.1093/bioinformatics/btt086. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 11.Jain C, Rodriguez-R LM, Phillippy AM, Konstantinidis KT, Aluru S. 2018. High-throughput ANI analysis of 90K prokaryotic genomes reveals clear species boundaries. Nat Commun 9:5114. doi: 10.1038/s41467-018-07641-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 12.Pritchard L, Glover RH, Humphris S, Elphinstone JG, Toth IK, Thomas CM, Pallen MJ, Moiemen NS, Bamford A, Oppenheim B, Loman NJ, Foster JT, Wagner DM, Okinaka RT, Sim SH, Pearson O, Wu Z, Chang J, Kaul R, Hoffmaster AR, Brettin TS, Robison RA, Mayo M, Gee JE, Tan P, Currie BJ, Keim P, He Y, Hines HM, Ibrahim N, Jackson LM, Jaiswal P, James-Zorn C, Kohler S, Lecointre G, Lapp H, Lawrence CJ, Le Novere N, Lundberg JG, Macklin J, Mast AR, Midford PE, Miko I, Mungall CJ, Oellrich A, Osumi-Sutherland D, Parkinson H, Ramirez MJ, Richter S, Robinson PN, Ruttenberg A, Schulz KS, Segerdell E, Seltmann KC, Sharkey MJ, Smith AD, Smith B, Specht CD, Squires RB, Thacker RW, Thessen A, Fernandez-Triana J, Vihinen M, Vize PD, Vogt L, Wall CE, Walls RL, Westerfeld M, Wharton RA, Wirkner CS, Woolley JB, Yoder MJ, Zorn AM, Mabee P. 2016. Genomics and taxonomy in diagnostics for food security: soft-rotting enterobacterial plant pathogens. Anal Methods 8:12–24. doi: 10.1039/C5AY02550H. [DOI] [Google Scholar]
- 13.Tatusova T, DiCuccio M, Badretdin A, Chetvernin V, Nawrocki EP, Zaslavsky L, Lomsadze A, Pruitt KD, Borodovsky M, Ostell J. 2016. NCBI Prokaryotic Genome Annotation Pipeline. Nucleic Acids Res 44:6614–6624. doi: 10.1093/nar/gkw569. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 14.Connor TR, Loman NJ, Thompson S, Smith A, Southgate J, Poplawski R, Bull MJ, Richardson E, Ismail M, Elwood-Thompson S, Kitchen C, Guest M, Bakke M, Sheppard SK, Pallen MJ. 2016. CLIMB (the Cloud Infrastructure for Microbial Bioinformatics): an online resource for the medical microbiology community. Microb Genomics 2:e000086. doi: 10.1099/mgen.0.000086. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 15.Jenner M, Jian X, Dashti Y, Masschelein J, Hobson C, Roberts DM, Jones C, Harris S, Parkhill J, Raja HA, Oberlies NH, Pearce CJ, Mahenthiralingam E, Challis GL. 2019. An unusual Burkholderia gladioli double chain-initiating nonribosomal peptide synthetase assembles “fungal” icosalide antibiotics. Chem Sci 10:5489–5494. doi: 10.1039/c8sc04897e. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 16.Mahenthiralingam E, Song L, Sass A, White J, Wilmot C, Marchbank A, Boaisha O, Paine J, Knight D, Challis GL. 2011. Enacyloxins are products of an unusual hybrid modular polyketide synthase encoded by a cryptic Burkholderia ambifaria genomic island. Chem Biol 18:665–677. doi: 10.1016/j.chembiol.2011.01.020. [DOI] [PubMed] [Google Scholar]
- 17.Masschelein J, Sydor PK, Hobson C, Howe R, Jones C, Roberts DM, Yap ZL, Parkhill J, Mahenthiralingam E, Challis GL. 2019. A dual transacylation mechanism for polyketide synthase chain release in enacyloxin antibiotic biosynthesis. Nat Chem 11:906–912. doi: 10.1038/s41557-019-0309-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 18.Kosol S, Gallo A, Griffiths D, Valentic TR, Masschelein J, Jenner M, de los Santos ELC, Manzi L, Sydor PK, Rea D, Zhou S, Fülöp V, Oldham NJ, Tsai SC, Challis GL, Lewandowski JR. 2019. Structural basis for chain release from the enacyloxin polyketide synthase. Nat Chem 11:913–923. doi: 10.1038/s41557-019-0335-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 19.Mullins AJ, Murray JAH, Bull MJ, Jenner M, Jones C, Webster G, Green AE, Neill DR, Connor TR, Parkhill J, Challis GL, Mahenthiralingam E. 2019. Genome mining identifies cepacin as a plant-protective metabolite of the biopesticidal bacterium Burkholderia ambifaria. Nat Microbiol 4:996–1005. doi: 10.1038/s41564-019-0383-z. [DOI] [PMC free article] [PubMed] [Google Scholar]
Associated Data
This section collects any data citations, data availability statements, or supplementary materials included in this article.
Data Availability Statement
The genome sequences in this announcement have been deposited in the European Nucleotide Archive (ENA) under the project accession number PRJEB35318. The BioSample accession numbers for the 454 genome assemblies are ERS4267623 to ERS4268076. The Illumina paired-end read data associated with these genome assemblies have been deposited under the project accession number PRJEB9765, with the exception of Burkholderia diffusa LMG 29043, which is available under project accession number PRJEB35318.