PIM1 upregulates c-Kit protein expression. (a) Immunoblot analysis showing expression of c-Kit in PIM1 vs. NTg whole heart lysates with quantification below. N = 3, Error bars represent SEM, * p < 0.05 vs. untreated as measured by Student t test. (b) Immunoblot analysis showing the expression of c-Kit in PIM-TKO vs. NTg whole heart lysates with quantification shown below. N = 3, error bars represent SEM, * p < 0.05 vs. untreated as measured by Student t test. (c) Immunoblot analysis showing c-Kit expression in cardiomyocytes isolated from PIM1-overexpressing hearts vs. NTg with quantification on the right. N = 3, error bars represent SEM, * p < 0.05 vs. untreated as measured by Student t test. (d) Gene expression of c-Kit in cardiomyocytes isolated from PIM1 and NTg hearts as revealed by qPCR. N = 3, Error bars represent SEM.