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. 2020 Aug 25;10(9):1233. doi: 10.3390/biom10091233

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Regional seeding activity detected by RT-QuIC. (A) A 3R/4R tau RT-QuIC assay was used to measure seeding activity in the frontal cortex and cerebellum in AD cases. Endpoint dilution analysis was used to determine seeding dose (SD₅₀)/mg brain tissue, shown here on a logarithmic scale. Brain regions are colored as per average log SD₅₀. Figure created with Biorender.com. (B) A bar graph shows the average logarithmic SD₅₀ values determined in distinct brain regions as indicated from two AD cases.