Table 1.

Mutations to residues far away from BH3 domain binding site impact vBcl2 function. ${}^{†}$ refers to the mutation that destabilizes the protein (see text below) and, hence, no Isothermal Titration Calorimetry (ITC) measurements are available.

	$K_d$ ($\mathsf{\mu }$M)	$K_a$ ($1/M$)	$\mathsf{\Delta }$H (kJ/mol)	$\mathsf{\Delta }$G (kJ/mol)	$\mathsf{\Delta }$S (J/K·mol)	Distance (Å)
WT	$0.84\pm 0.0$	$120.0\pm 24\times {10}^4$	$-53.6\pm 3.9$	$-33.4\pm 0.04$	$-70.5\pm 13.6$	-
F48A	$63.4\pm 2.05$	$15.8\pm 0.5\times {10}^4$	$-36.1\pm 3.5$	$-23.1\pm 0.07$	$-45.4\pm 12.7$	9.4
A63K	$6.5\pm 0.04$	$16.0\pm 0.9\times {10}^4$	$-39.7\pm 0.45$	$-28.5\pm 0.02$	$-38.9\pm 1.60$	11.9
Y56A	$3.38\pm 0.4$	$30.0\pm 3\times {10}^4$	$-37.8\pm 7.9$	$-30.1\pm 0.2$	$-27.0\pm 28.5$	9.7
F17A ${}^{†}$	N/A	N/A	N/A	N/A	N/A	19.7