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Validation of genes and LTB4 levels in adipose tissue from obese B6 mice. The expression of genes was validated by Q-PCR. (A) Gene expression of inflammatory markers Alox5, Ltb4r, Myd88, Stat1, Ccr5, and Card11; data are expressed as mean ± SEM, p ≤ 0.05, n = 6. (B) LTB4 levels in adipose tissue. Data are expressed as mean ± SEM, * p ≤ 0.05, n = 6; HF vs HF-EPA.
