Skip to main content
. 2020 Sep 21;9(9):2133. doi: 10.3390/cells9092133

Figure 3.

Figure 3

Knockdown of NRF2 enhances RANKL-inducible MYC expression. Mouse OCPs were transfected with negative control (NC) or NRF2-specific small interfering RNAs (siRNAs) and stimulated with RANKL (50 ng/mL) for the indicated time points. (A) The expression of NRF2 was determined using immunoblot with nuclear lysates from transfected cells. Lamin B served as a loading control. Data are representative of two experiments from five mice. (B) The mRNA expression of Myc (relative to the Hprt housekeeping gene) at 19 h following RANKL stimulation (n = 5). (C) The expression of MYC using immunoblot with nuclear lysates at 24 h following RANKL stimulation. Lamin B served as a loading control. Data are representative of two experiments from five mice. (D) Signal intensity of the c-Myc immunoblot in C quantified using densitometry and normalized to Lamin B and to unstimulated NC control (n = 5). All data are shown as mean ± s.e.m. * p < 0.05 and ** p < 0.01 using two-way ANOVA.