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. 2020 Sep 2;10(9):1264. doi: 10.3390/biom10091264

Figure 1.

Figure 1

Co-expressed acid-sensing ion channel subunits 1a and 3 (ASIC1a and ASIC3) formed heteromeric complex in Chinese hamster ovary (CHO) cells. (A) Immunostaining shows ASIC1 and ASIC3 expression in transfected cells. CHO cells were transfected with ASIC1a, AU1-ASIC3, or ASIC1a + AU1-ASIC3 (1:2 ratio). CHO cells were stained for AU1-ASIC3 (green) and ASIC1 (red) as described in the Materials and Methods section. Note that, when co-transfected into the CHO cell (right panel), most of the ASIC1a expressing cells also were positive for AU1-ASIC3 staining; (B) ASIC1a and ASIC3 co-immunoprecipitate from transfected cells. CHO cells were transfected with ASIC1a, AU1-ASIC3, or ASIC1a + AU1-ASIC3 (1:2 ratio). Immunoprecipitation was performed using a goat anti-ASIC1 or goat anti-AU1-ASIC3. Total lysate and immunoprecipitation (IP) fractions were blotted for ASIC1 (rabbit) or AU1-ASIC3 (mouse) as indicated.