Table 1.
Data Collection | Value |
---|---|
Voltage (kV) | 300 |
Total dose (e−/Å2) | 56.4 |
Nominal magnification | 81,000 |
Super-resolution pixel size (Å) | 0.536 |
Number of movies | 2175 |
Number of frames/movies | 40 |
Intended defocus (μm) | 0.5–2 |
Image processing | |
Particles picked | 1,535,389 |
Final number of particles | 112,241 |
Refined resolution (Å) | 2.87 |
Refinement | |
Map CC | 0.8576 |
All-atom clashscore | 5 |
Rotamer outliers (%) | 0 |
Ramachandran plot | |
Favored (%) | 96.56 |
Allowed (%) | 3.44 |
Outliers (%) | 0 |