Table 2.
Diurnal differences in myofibrillar proteoform abundance.
| Spot # | UniProt ID | Protein Name | MOWSE | % Seq | Anova (p) | FC | Morning Mean | Morning SD | Evening Mean | Evening SD |
|---|---|---|---|---|---|---|---|---|---|---|
| 335 | PYGM | Glycogen phosphorylase, muscle form | 1938 | 53 | 0.0155 | 1.43 | 245 | 44 | 350 | 104 |
| 163 | MYPC1 | Myosin-binding protein C, slow-type | 2135 | 42 | 0.0226 | 1.80 | 343 | 86 | 617 | 357 |
| 703 | MYBPH | Myosin-binding protein H | 425 | 23 | 0.0313 | 1.17 | 133 | 28 | 114 | 26 |
| 874 | ENOB | Beta-enolase | 1040 | 55 | 0.0335 | 1.24 | 814 | 199 | 1007 | 174 |
| 1046 | TNNT1 | Troponin T, slow skeletal muscle | 738 | 32 | 0.0433 | 1.22 | 239 | 71 | 196 | 45 |
| 154 | NEBU | Nebulin | 964 | 6 | 0.0435 | 1.48 | 959 | 352 | 648 | 252 |
| 391 | MYH2 | Myosin-2 | 1510 | 18 | 0.0546 | 1.37 | 239 | 56 | 329 | 158 |
| 881 | KCRM | Creatine kinase M-type | 922 | 54 | 0.0570 | 1.17 | 535 | 74 | 625 | 118 |
| 795 | ACTS | Actin, alpha skeletal muscle | 1011 | 54 | 0.0620 | 1.47 | 271 | 114 | 185 | 56 |
| 742 | ACTN2 | Alpha-actinin-2 | 80 | 4 | 0.0727 | 1.25 | 510 | 102 | 408 | 134 |
| 1059 | TNNT3 | Troponin T, fast skeletal muscle | 394 | 24 | 0.0735 | 1.32 | 244 | 84 | 186 | 54 |
| 992 | ALDOA | Fructose-bisphosphate aldolase A | 733 | 58 | 0.0929 | 1.19 | 472 | 126 | 559 | 138 |
| 903 | ACTS | Actin, alpha skeletal muscle | 1009 | 62 | 0.0931 | 1.67 | 16,872 | 10,694 | 10,109 | 2314 |
| 135 | MYPC2 | Myosin-binding protein C, fast-type | 2015 | 45 | 0.0978 | 1.23 | 168 | 54 | 137 | 41 |
| 1357 | KCRM | Creatine kinase M-type | 466 | 32 | 0.0978 | 1.43 | 2542 | 1135 | 1782 | 577 |
| 686 | NEBU | Nebulin | 2181 | 13 | 0.1017 | 1.17 | 336 | 66 | 394 | 69 |
| 386 | MYH2 | Myosin-2 | 1266 | 13 | 0.1048 | 1.27 | 233 | 78 | 295 | 104 |
| 739 | DESM | Desmin | 1884 | 63 | 0.1072 | 1.20 | 1176 | 181 | 979 | 292 |
| 1043 | TNNT1 | Troponin T, slow skeletal muscle | 599 | 33 | 0.1143 | 1.28 | 884 | 293 | 689 | 278 |
| 596 | MYH7 | Myosin-7 | 1229 | 17 | 0.1321 | 1.15 | 259 | 66 | 298 | 49 |
| 392 | MYH2 | Myosin-2 | 709 | 12 | 0.1328 | 1.17 | 109 | 44 | 128 | 36 |
| 390 | MYH2 | Myosin-2 | 1367 | 14 | 0.1481 | 1.28 | 177 | 40 | 227 | 89 |
| 855 | ACTS | Actin, alpha skeletal muscle | 1319 | 65 | 0.1531 | 1.25 | 18,032 | 6216 | 14,461 | 4631 |
| 158 | MYPC1 | Myosin-binding protein C, slow-type | 2460 | 49 | 0.1606 | 1.32 | 547 | 224 | 721 | 297 |
| 387 | MYH2 | Myosin-2 | 915 | 12 | 0.1636 | 1.24 | 173 | 85 | 214 | 71 |
| 685 | KPYM | Pyruvate kinase isozymes M1/M2 | 1329 | 51 | 0.1662 | 1.17 | 398 | 68 | 466 | 112 |
| 731 | DESM | Desmin | 2161 | 72 | 0.1818 | 1.16 | 2919 | 408 | 2527 | 728 |
| 153 | MYPC1 | Myosin-binding protein C, slow-type | 2648 | 51 | 0.1857 | 1.34 | 518 | 218 | 694 | 314 |
| 344 | PYGM | Glycogen phosphorylase, muscle form | 1560 | 43 | 0.1876 | 1.53 | 198 | 48 | 304 | 242 |
| 741 | DESM | Desmin | 1927 | 67 | 0.1927 | 1.17 | 3290 | 778 | 2811 | 997 |
| 544 | ALBU | Serum albumin | 1539 | 56 | 0.2047 | 1.25 | 682 | 203 | 852 | 348 |
| 389 | TNNT1 | Troponin T, slow skeletal muscle | 153 | 23 | 0.2070 | 1.16 | 558 | 103 | 645 | 155 |
| 1065 | TNNT3 | Troponin T, fast skeletal muscle | 421 | 26 | 0.2103 | 1.18 | 542 | 124 | 460 | 117 |
| 164 | MYPC1 | Myosin-binding protein C, slow-type | 2581 | 51 | 0.2105 | 1.25 | 337 | 57 | 423 | 190 |
| 751 | TBA4A | Tubulin alpha-4A | 467 | 29 | 0.2232 | 1.14 | 312 | 60 | 274 | 43 |
| 997 | ALDOA | Fructose-bisphosphate aldolase A | 812 | 60 | 0.2234 | 1.21 | 652 | 188 | 789 | 289 |
| 546 | ALBU | Serum albumin | 1622 | 59 | 0.2244 | 1.34 | 1381 | 468 | 1850 | 766 |
| 812 | ATPA | ATP synthase subunit alpha, mitochondrial | 1275 | 45 | 0.2274 | 1.15 | 688 | 164 | 790 | 219 |
| 150 | MYPC1 | Myosin-binding protein C, slow-type | 2849 | 50 | 0.2300 | 1.30 | 442 | 236 | 575 | 280 |
| 813 | ATPA | ATP synthase subunit alpha, mitochondrial | 963 | 42 | 0.2328 | 1.10 | 755 | 167 | 829 | 176 |
| 1606 | VIME | Vimentin | 1861 | 69 | 0.2342 | 1.35 | 707 | 339 | 955 | 389 |
| 1105 | TPM2 | Tropomyosin beta chain | 567 | 32 | 0.2501 | 1.19 | 454 | 120 | 381 | 80 |
| 1251 | CAH3 | Carbonic anhydrase 3 | 419 | 44 | 0.2622 | 1.14 | 829 | 240 | 944 | 230 |
| 878 | ENOB | Beta-enolase | 1196 | 53 | 0.2624 | 1.19 | 1109 | 283 | 1325 | 358 |
| 616 | CATA | Catalase | 710 | 38 | 0.2974 | 1.77 | 832 | 103 | 1476 | 1963 |
| 941 | KCRM | Creatine kinase M-type | 890 | 54 | 0.3013 | 1.14 | 1295 | 299 | 1480 | 439 |
| 947 | KCRM | Creatine kinase M-type | 1295 | 57 | 0.3075 | 1.14 | 6801 | 1461 | 7786 | 1689 |
| 1057 | ACTC | Actin, alpha cardiac muscle 1 | 52 | 11 | 0.3596 | 1.16 | 329 | 118 | 383 | 185 |
| 505 | HSP7C | Heat shock cognate 71 kDa protein | 1246 | 39 | 0.3731 | 1.19 | 1386 | 475 | 1643 | 850 |
| 500 | HSP71 | Heat shock 70 kDa protein 1A/1B | 1470 | 46 | 0.3816 | 1.02 | 583 | 99 | 570 | 136 |
| 1085 | TPM3 | Tropomyosin alpha-3 chain | 1478 | 72 | 0.3846 | 1.10 | 37,171 | 11,538 | 33,691 | 9083 |
| 1603 | ALBU | Serum albumin | 1625 | 58 | 0.3869 | 1.22 | 714 | 404 | 867 | 335 |
| 1097 | TNNT1 | Troponin T, slow skeletal muscle | 576 | 32 | 0.3914 | 1.20 | 1448 | 656 | 1207 | 609 |
| 553 | ALBU | Serum albumin | 1838 | 60 | 0.3938 | 1.29 | 4881 | 2765 | 6293 | 3338 |
| 902 | ACTS | Actin, alpha skeletal muscle | 962 | 63 | 0.4326 | 1.25 | 8992 | 5539 | 7196 | 2076 |
| 1374 | MYL3 | Myosin light chain 3 | 856 | 77 | 0.4537 | 1.10 | 8098 | 3473 | 7382 | 2866 |
| 1078 | MYOZ1 | Myozenin-1 | 848 | 85 | 0.4556 | 1.00 | 401 | 76 | 402 | 239 |
| 1336 | TPIS | Triosephosphate isomerase | 929 | 76 | 0.4585 | 1.04 | 316 | 48 | 304 | 100 |
| 1103 | KCRM | Creatine kinase M-type | 418 | 29 | 0.4598 | 1.40 | 623 | 607 | 446 | 123 |
| 944 | KCRM | Creatine kinase M-type | 1100 | 53 | 0.4644 | 1.12 | 3853 | 690 | 4310 | 1195 |
| 900 | ACTS | Actin, alpha skeletal muscle | 415 | 39 | 0.4649 | 1.21 | 3869 | 2169 | 3198 | 713 |
| 1272 | MYOZ1 | Myozenin-1 | 154 | 29 | 0.4785 | 1.05 | 355 | 88 | 373 | 47 |
| 532 | PGM1 | Phosphoglucomutase-1 | 876 | 37 | 0.4889 | 1.09 | 446 | 125 | 409 | 80 |
| 106 | MYH2 | Myosin-2 | 3726 | 33 | 0.4924 | 1.59 | 550 | 340 | 874 | 888 |
| 1090 | TNNT3 | Troponin T, fast skeletal muscle | 520 | 32 | 0.5034 | 1.12 | 591 | 178 | 528 | 89 |
| 333 | PYGM | Glycogen phosphorylase, muscle form | 1611 | 47 | 0.5039 | 1.21 | 164 | 33 | 198 | 95 |
| 1503 | KCRM | Creatine kinase M-type | 151 | 13 | 0.5085 | 1.06 | 1063 | 345 | 1007 | 590 |
| 1032 | TNNT3 | Troponin T, fast skeletal muscle | 491 | 27 | 0.5192 | 1.23 | 1032 | 534 | 837 | 258 |
| 943 | KCRM | Creatine kinase M-type | 1126 | 59 | 0.5267 | 1.06 | 2690 | 574 | 2846 | 551 |
| 1056 | TNNT1 | Troponin T, slow skeletal muscle | 687 | 37 | 0.5340 | 1.13 | 8515 | 4319 | 7506 | 4221 |
| 1049 | TNNT1 | Troponin T, slow skeletal muscle | 652 | 36 | 0.5550 | 1.10 | 1792 | 601 | 1630 | 818 |
| 165 | MYPC1 | Myosin-binding protein C, slow-type | 2366 | 51 | 0.5618 | 1.14 | 323 | 69 | 368 | 161 |
| 1266 | TPIS | Triosephosphate isomerase | 932 | 65 | 0.5624 | 1.07 | 1389 | 280 | 1489 | 354 |
| 1132 | MYH2 | Myosin-2 | 616 | 8 | 0.5679 | 1.05 | 1154 | 324 | 1209 | 255 |
| 604 | MYH2 | Myosin-2 | 1104 | 13 | 0.5805 | 1.05 | 199 | 43 | 189 | 41 |
| 121 | MYH2 | Myosin-2 | 4044 | 37 | 0.5910 | 1.51 | 691 | 599 | 1041 | 1196 |
| 455 | KPYM | Pyruvate kinase isozymes M1/M2 | 815 | 36 | 0.6060 | 1.06 | 856 | 158 | 810 | 117 |
| 550 | ALBU | Serum albumin | 1852 | 65 | 0.6109 | 1.23 | 2514 | 1360 | 3093 | 1897 |
| 1074 | G3P | Glyceraldehyde-3-phosphate dehydrogenase | 1068 | 54 | 0.6170 | 1.03 | 3635 | 771 | 3532 | 1105 |
| 925 | ACTS | Actin, alpha skeletal muscle | 1277 | 63 | 0.6537 | 1.05 | 73,798 | 17,469 | 77,544 | 17,257 |
| 123 | MYH2 | Myosin-2 | 3325 | 34 | 0.6580 | 1.31 | 819 | 851 | 1072 | 1290 |
| 798 | ATPB | ATP synthase subunit beta, mitochondrial | 1882 | 68 | 0.6678 | 1.13 | 947 | 356 | 1069 | 547 |
| 1083 | TNNT3 | Troponin T, fast skeletal muscle | 583 | 34 | 0.6867 | 1.12 | 2406 | 775 | 2689 | 1066 |
| 136 | MYPC2 | Myosin-binding protein C, fast-type | 1838 | 41 | 0.6868 | 1.07 | 223 | 72 | 207 | 45 |
| 1073 | TNNT3 | Troponin T, fast skeletal muscle | 482 | 26 | 0.6887 | 1.09 | 1620 | 579 | 1492 | 603 |
| 1048 | TNNT1 | Troponin T, slow skeletal muscle | 738 | 32 | 0.6912 | 1.25 | 3355 | 2869 | 2681 | 1052 |
| 471 | MYH2 | Myosin-2 | 1204 | 15 | 0.6940 | 1.09 | 202 | 91 | 186 | 98 |
| 934 | KCRM | Creatine kinase M-type | 1134 | 54 | 0.6981 | 1.03 | 1253 | 233 | 1222 | 210 |
| 1115 | MYOZ1 | Myozenin-1 | 378 | 30 | 0.7011 | 1.05 | 1462 | 416 | 1393 | 415 |
| 904 | ACTS | Actin, alpha skeletal muscle | 877 | 55 | 0.7143 | 1.01 | 9431 | 1361 | 9383 | 3034 |
| 443 | MYH2 | Myosin-2 | 978 | 13 | 0.7267 | 1.05 | 331 | 103 | 348 | 96 |
| 1381 | KCRM | Creatine kinase M-type | 469 | 28 | 0.7267 | 1.08 | 24,048 | 9478 | 22,334 | 6612 |
| 115 | MYH7 | Myosin-7 | 4288 | 35 | 0.7551 | 1.14 | 2646 | 2960 | 3028 | 3141 |
| 1061 | TNNT3 | Troponin T, fast skeletal muscle | 457 | 36 | 0.7595 | 1.28 | 540 | 382 | 424 | 155 |
| 1044 | ACTC | Actin, alpha cardiac muscle 1 | 342 | 28 | 0.7668 | 1.14 | 2912 | 1480 | 2552 | 917 |
| 1080 | ACTC | Actin, alpha cardiac muscle 1 | 183 | 23 | 0.7707 | 1.06 | 4741 | 1932 | 4479 | 2310 |
| 270 | ACTN2 | Alpha-actinin-2 | 3558 | 69 | 0.7779 | 1.08 | 2145 | 422 | 2326 | 812 |
| 271 | ACTN2 | Alpha-actinin-2 | 3354 | 71 | 0.7866 | 1.04 | 679 | 243 | 706 | 256 |
| 274 | ACTN2 | Alpha-actinin-2 | 3445 | 69 | 0.7899 | 1.07 | 1058 | 239 | 1128 | 390 |
| 187 | MYH7 | Myosin-7 | 3534 | 31 | 0.7939 | 1.55 | 6318 | 8061 | 4082 | 3064 |
| 1051 | TNNT3 | Troponin T, fast skeletal muscle | 507 | 36 | 0.8007 | 1.11 | 1454 | 657 | 1312 | 446 |
| 1108 | MYOZ1 | Myozenin-1 | 708 | 50 | 0.8010 | 1.02 | 3435 | 1569 | 3513 | 1097 |
| 332 | PYGM | Glycogen phosphorylase, muscle form | 1651 | 44 | 0.8203 | 1.01 | 295 | 50 | 299 | 93 |
| 330 | TPM1 | Tropomyosin alpha-1 chain | 947 | 47 | 0.8216 | 1.07 | 1964 | 874 | 1834 | 716 |
| 1100 | TNNT3 | Troponin T, fast skeletal muscle | 474 | 37 | 0.8243 | 1.12 | 4641 | 2707 | 4144 | 1391 |
| 1095 | TNNT3 | Troponin T, fast skeletal muscle | 452 | 28 | 0.8373 | 1.09 | 1477 | 719 | 1355 | 374 |
| 499 | HSP71 | Heat shock 70 kDa protein 1A/1B | 1070 | 39 | 0.8419 | 1.02 | 516 | 114 | 508 | 118 |
| 980 | TNNT1 | Troponin T, slow skeletal muscle | 696 | 35 | 0.8427 | 1.01 | 1131 | 271 | 1146 | 265 |
| 1198 | LDB3 | LIM domain-binding protein 3 | 609 | 17 | 0.8445 | 1.01 | 2437 | 827 | 2415 | 1038 |
| 899 | DESM | Desmin | 198 | 11 | 0.8495 | 1.08 | 1329 | 574 | 1230 | 235 |
| 1129 | VDAC1 | Voltage-dependent anion-selective channel protein 1 | 612 | 59 | 0.8774 | 1.10 | 383 | 206 | 347 | 82 |
| 1552 | HERC1 | Probable E3 ubiquitin-protein ligase HERC1 | 52 | 2 | 0.8852 | 1.06 | 1557 | 547 | 1652 | 1207 |
| 1101 | TNNT3 | Troponin T, fast skeletal muscle | 540 | 33 | 0.9155 | 1.01 | 1068 | 440 | 1077 | 383 |
| 477 | MYH2 | Myosin-2 | 2036 | 26 | 0.9173 | 1.08 | 564 | 398 | 609 | 497 |
| 533 | MYH2 | Myosin-2 | 1084 | 13 | 0.9250 | 1.27 | 365 | 409 | 289 | 165 |
| 1397 | ENOB | Beta-enolase | 266 | 26 | 0.9287 | 1.02 | 12,335 | 3415 | 12,540 | 3761 |
| 535 | MYH2 | Myosin-2 | 1195 | 17 | 0.9394 | 1.23 | 456 | 482 | 370 | 163 |
| 1451 | TNNI2 | Troponin I, fast skeletal muscle | 378 | 50 | 0.9545 | 1.02 | 229 | 127 | 225 | 130 |
| 479 | MYH2 | Myosin-2 | 1359 | 18 | 0.9612 | 1.04 | 543 | 385 | 563 | 481 |
| 275 | ACTN2 | Alpha-actinin-2 | 3512 | 71 | 0.9648 | 1.04 | 925 | 221 | 965 | 355 |
| 534 | MYH2 | Myosin-2 | 1360 | 16 | 0.9683 | 1.14 | 588 | 587 | 515 | 327 |
| 336 | PYGM | Glycogen phosphorylase, muscle form | 1778 | 50 | 0.9720 | 1.02 | 310 | 96 | 305 | 69 |
| 1251 | HBB | Hemoglobin subunit beta | 441 | 60 | 0.9780 | 1.00 | 834 | 137 | 837 | 171 |
| 195 | MYH7 | Myosin-7 | 4307 | 32 | 0.9894 | 1.34 | 2446 | 3633 | 1821 | 1188 |
| 1060 | TNNT3 | Troponin T, fast skeletal muscle | 481 | 26 | 0.9915 | 1.01 | 2012 | 749 | 2001 | 801 |
| 1034 | TPM2 | Tropomyosin beta chain | 1450 | 69 | 0.9963 | 1.01 | 22,483 | 6641 | 22,339 | 5236 |
Spot # correspond with the 2D gel map (Figure 4). UniProt identifier and protein name based on peptide mass spectrometry of in-gel protein digests searched against human entries in the SwissProt database using Mascot. The probability-based molecular weight search score (MOWSE) and percentage of protein sequence identified are presented. Spot abundances (AU) in the morning and evening are presented as mean (SD) and statistical difference (P) was determined by within-subject ANOVA (n = 10). Fold change (FC) is spot abundance in the evening compared to the morning condition.