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. 2020 Sep 19;25(18):4296. doi: 10.3390/molecules25184296

Table 1.

Primers used for gene expression analysis.

Gene Primer Sequence (F and R)
5′ → 3′
Product Length (bp) Accession N. Reference
ZO-1 F: CGGGACTGTTGGTATTGGCTAGA
R: GGCCAGGGCCATAGTAAAGTTTG
184 NM_001301025.3 [39]
OCCL F: TCCTATAAATCCACGCCGGTTC
R: CTCAAAGTTACCACCGCTGCTG
105 NM_001205254.2 [39]
TRPV1 F: GACCACCTGGAACACCAACG
R: TGAGCAGACTGCCTATCTCG
177 NM_080704.3 [40]
TRPV3 F: GAGCAGATTCCGGATGGGA
R: CCGCAAACACAGTCGGAAA
64 NM_001258205.1 [41]
RPLP0 F: GCAATGTTGCCAGTGTCTG
R: GCCTTGACCTTTTCAGCAA
142 NM_001002.3 [42]
GAPDH F: TGCACCACCAACTGCTTAGC
R: GGCATGGACTGTGGTCATGAG
87 NM_02046 [43]

F = forward; R = reverse; ZO-1 = zonula occludens-1; OCCL = occludin; TRPV1 = Transient Receptor Potential Vanilloid 1; TRPV3 = Transient Receptor Potential Vanilloid 3; OR1G1 = Olfactory Receptor Family 1 Subfamily G Member 1; RPLP0 = Ribosomal Protein Lateral Stalk Subunit P0; GAPDH = Glyceraldehyde 3-phosphate dehydrogenase.