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. 2020 Sep 10;25(18):4138. doi: 10.3390/molecules25184138

Figure 6.

Figure 6

Effect of hemin, SFN, QUE and 3,4HPAA in complex I and II activities. Cells were incubated for 72 h with vehicle (0.4% DMSO), 1 μM SFN, 10 μM QUE or 2.6 μM 3,4HPAA in the absence or presence of 10 μM hemin. Complex I activity was measured in (A) RKO cells; and (B) CCD841 cells and results were expressed as nmol of oxidized NADH/minute/mg of protein. Complex II activity was measured in (C) RKO cells; and (D) CCD841 cells and results were expressed as nmol of reduced DCIP/minute/mg of protein. Values are expressed as mean ± SEM, from three independent culture preparations. For all bars with the same letter, the difference between the means is not statistically significant. Values with different letters indicate significant differences (p < 0.05) between bars (two-way ANOVA, Bonferroni post-test). 3,4HPAA, 3,4-dihydroxyphenylacetic acid; DCIP, 2,6-dichlorophenolin-dophenol; QUE, quercetin; SFN, sulforaphane.