Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens, male) | DU145 | ATCC | cat#:HTB-81; RRID:CVCL_0105 | |
| Cell line (H. sapiens, male) | Wild-type HCT116 cells | Horizon Discovery | cat# HD R02-019; RRID:CVCL_HD76 | |
| Cell line (H. sapiens, male) | DICEREx5 HCT116 cells | Horizon Discovery | cat# HD R02-019; RRID:CVCL_HD76 | |
| Recombinant DNA reagent | psiCHECK-2+PTEN3’UTR | Addgene | plasmid# 50936; RRID:Addgene_50936 | |
| Recombinant DNA reagent | SERINC1 3’UTR | doi:10.1016/j.cell.2011.09.029 | Shared by Dr. Pier Paolo Pandolfi, Beth Israel Deaconess Medical Center | |
| Recombinant DNA reagent | VAPA 3’UTR1 | doi:10.1016/j.cell.2011.09.029 | Shared by Dr. Pier Paolo Pandolfi, Beth Israel Deaconess Medical Center | |
| Recombinant DNA reagent | VAPA 3’UTR2 | doi:10.1016/j.cell.2011.09.029 | Shared by Dr. Pier Paolo Pandolfi, Beth Israel Deaconess Medical Center | |
| Recombinant DNA reagent | CNOT6L 3’UTR1 | doi:10.1016/j.cell.2011.09.029 | Shared by Dr. Pier Paolo Pandolfi, Beth Israel Deaconess Medical Center | |
| Recombinant DNA reagent | CNOT6L 3’UTR2 | doi:10.1016/j.cell.2011.09.029 | Shared by Dr. Pier Paolo Pandolfi, Beth Israel Deaconess Medical Center | |
| Recombinant DNA reagent | PTEN 3’UTR | doi:10.1016/j.cell.2011.09.029 | Shared by Dr. Pier Paolo Pandolfi, Beth Israel Deaconess Medical Center | |
| Sequence-based reagent | siGlo RISC-free siRNA | Dharmacon | cat#:D-001600–01 | |
| Sequence-based reagent | siGENOME non-targeting siRNA | Dharmacon | cat#:D-001210–02 | |
| Sequence-based reagent | siGENOME SERINC1 | Dharmacon | cat# M-010725–00 | |
| Sequence-based reagent | siGENOME ZNF460 | Dharmacon | cat# M-032012–01 | |
| Sequence-based reagent | siGENOME VAPA | Dharmacon | cat# M-021382–01 | |
| Sequence-based reagent | siGENOME CNOT6L | Dharmacon | cat# M-016411–01 | |
| Sequence-based reagent | siGENOME PTEN | Dharmacon | M-003023–02 | |
| Sequence-based reagent | TaqMan probe SERINC1 | Thermo Fisher Scientific | Hs00380375_m1 | |
| Sequence-based reagent | TaqMan probe ZNF460 | Thermo Fisher Scientific | Hs01104252_m1 | |
| Sequence-based reagent | TaqMan probe VAPA | Thermo Fisher Scientific | Hs00427749_m1 | |
| Sequence-based reagent | TaqMan probe CNOT6L | Thermo Fisher Scientific | Hs00375913_m1 | |
| Sequence-based reagent | TaqMan probe PTEN | Thermo Fisher Scientific | Hs02621230_s1 | |
| Sequence-based reagent | TaqMan probe PARD3 | Thermo Fisher Scientific | Hs00969077_m1 | |
| Antibody | rabbit anti-PTEN | Cell Signaling Technology | cat#:9559; clone:138G5; RRID:AB_390810 | 1:1000 dilution |
| Antibody | mouse anti-HSP90 | BD Biosciences | cat#:610419; clone:68; RRID:AB_397798 | 1:1000 dilution |
| Antibody | HRP-conjugated donkey anti-rabbit | GE Healthcare | cat#:NA934; RRID:AB_772206 | 1:2000 dilution |
| Antibody | HRP-conjugated rabbit anti-mouse | Abcam | cat#:ab6728; RRID:AB_955440 | 1:2000 dilution |
| Software, algorithm | Veritas Microplate Luminometer software | Turner BioSystems | part#:998–9100; RRID:SCR_018534 | |
| Software, algorithm | StepOne Plus Real-Time PCR software | Applied Biosystems | RRID:SCR_014281 | Version 2.3 |
| Software, algorithm | ImageJ | doi:10.1038/nmeth.2089 | RRID:SCR_003070 | Version 1.50a |
| Software, algorithm | Gen5 software | BioTek Instruments | RRID:SCR_017317 | Version 2.05.5 |
| Software, algorithm | R Project for statistical computing | https://www.r-project.org | RRID:SCR_001905 | Version 3.5.1 |