TABLE 3.
The combined insertion sites found in coding regions in the four separate analyses of a rough-colony M. abscessus transposon-mutant librarya
| Hit sequence (12N + TA + 12N) | Locus | Gene length | Insertion point (base within gene) | Strand orientation |
|---|---|---|---|---|
| CTCCCCGACGACTACAGCTTTACGCT | MAB_0023 | 918 | 711 | + |
| GGGACGGGTACTTATCGTGGGCAGCG | MAB_0062 | 1,404 | 548 | + |
| CCCGTCACGGTGTACTGCGGCTCGGG | MAB_0071c | 837 | 693 | − |
| GGGCGCGCAACATATACCGCTCGGCG | MAB_0121 | 327 | 137 | + |
| TGGCGGTCCGTATAACACCGCGATCG | MAB_0359 | 927 | 101 | + |
| ACAACAATCTCCTATACGGGCGATCC | MAB_0434c | 1,443 | 183 | − |
| CCTCACCGTACCTATCACTCCTGCGC | MAB_0495c | 423 | 130 | − |
| CAAGTCCGTATCTATCTACAGAAAGC | MAB_0501c | 1,647 | 999 | − |
| TGGCATGTCGACTACATGTTCATGCC | MAB_0659 | 870 | 297 | + |
| GGCCTGTGGACGTATCTGATGGGCCG | MAB_0677c | 972 | 717 | − |
| ATTTCCAAGGCGTACAGAACTATTCG | MAB_0737c | 378 | 169 | − |
| GTGACCGGCACCTACGACCGCAACTC | MAB_0841 | 993 | 576 | + |
| GGGCGGGAGGCCTACGCGGCCAAGCT | MAB_0962 | 2,403 | 570 | + |
| TCATTCAGGAGCTACGCGCCAAGCAG | MAB_0962 | 2,403 | 259 | + |
| ACCCGGGATCAATACTGCCCTCGGGC | MAB_0978 | 2,235 | 967 | + |
| CTGGCCGAGCCTTACCTCCTGGAGGG | MAB_1003c | 819 | 531 | − |
| ATCCAGTGCTACTACGGCTTCTACGC | MAB_1011c | 858 | 708 | − |
| ACCGCATCAACTTATGTACCCCCGAT | MAB_1011c | 858 | 15 | − |
| CGAATCGTGGCCTACGGTCGTGTCCG | MAB_1019c | 1,071 | 608 | − |
| GGAAAAGATACCTATAGCCGAGCAGA | MAB_1103 | 729 | 369 | + |
| CCCGCTGCCCTTTACTTATCCGCCTT | MAB_1304 | 1,248 | 269 | + |
| ACCAATATCTGCTACACCGGCTGCCG | MAB_1319 | 2,646 | 1,743 | + |
| TTCATCGCGCTATACCTGATCGATCA | MAB_1338 | 1,200 | 732 | + |
| CGATGGCCTCAATAAGGTCATCGCGG | MAB_1470c | 1,446 | 659 | − |
| CGGTTGACGCACTACATGGCGCTGAT | MAB_1638 | 648 | 417 | + |
| ACTCGTTACTTCTACGAGTGCTTCGA | MAB_1638 | 648 | 174 | + |
| GCTACCGGGCTATACGGCGGACGAAC | MAB_1638 | 648 | 27 | + |
| CCTGGGTAGTAATAGCATTCGTCGTC | MAB_1814 | 348 | 70 | + |
| TACCTGTATCACTACACCAGCGCTGC | MAB_1826 | 1,071 | 108 | + |
| GAGCTGGCGATGTACACGTTGTTCCT | MAB_1841c | 540 | 285 | − |
| GTCGGCCTGGAATACGACCCTGAATA | MAB_1851 | 1,140 | 159 | + |
| ACCTGCGACGCGTACTGCACGGTGAG | MAB_2054 | 468 | 250 | + |
| GGCACCAAGTACTACATCTCCGGTGT | MAB_2065c | 1,155 | 462 | − |
| GAACAGGAAACATACATCGATCTCGC | MAB_2174 | 363 | 24 | + |
| GATCGCCGCGCATATAACCCTGGGCG | MAB_2444c | 804 | 533 | − |
| CGGCCTATGCGGTATACGCGCTGCCC | MAB_2514c | 765 | 694 | − |
| GCACGCGCAAGCTACACATTCCCGTC | MAB_2545c | 984 | 436 | − |
| TGCCGCCAAACGTATTGGTGCGAGCC | MAB_2569c | 759 | 251 | − |
| ACGGACCGTCTGTACGACGCGGGGAT | MAB_2975c | 1,047 | 894 | − |
| GGCCGGCGCCGTTAAGTTCAAACGCA | MAB_3040c | 1,155 | 14 | − |
| CGTCGGGTCAAGTAACTGTTGATACG | MAB_3049c | 420 | 142 | − |
| ATCACCGCCATGTACGAAATGCACGC | MAB_3073 | 657 | 510 | + |
| CCCCATGACTACTACTGGCGAGCGGG | MAB_3076 | 1,365 | 51 | + |
| GCGGCACGGCAATACGTCCGCAAGAT | MAB_3121c | 270 | 78 | − |
| GATCGGCACTGTTATGTCGCGGTTGC | MAB_3543c | 708 | 599 | − |
| GGGTAACCTACGTACGCTATTGACCG | MAB_3651 | 4,554 | 2,003 | + |
| TCGGGGAAGGCATATGTGCAATGGGG | MAB_3728c | 447 | 414 | − |
| GATCTCCGGTGGTATGCGCAAGCGTG | MAB_3871c | 1,149 | 452 | − |
| TCCAAAGAGCTGTACGAGATCCGCAA | MAB_3871c | 1,149 | 249 | − |
| ATCCAGATCAGTTATGACGACCGCGG | MAB_3934c | 837 | 36 | − |
| CTGCAGGGCTGCTACAAGATCGCCCT | MAB_3972c | 1,512 | 744 | − |
| AATCAGAGTGAATACAGCCGTAAGCG | MAB_4088c | 870 | 303 | − |
| AATTGGGGGAGATACAAGCGGCCTTG | MAB_4098c | 7,746 | 1,327 | − |
| CTCATCCGGGTGTACGAGGCGCTGGC | MAB_4098c | 7,746 | 6,702 | − |
| GCGTACATGACCTATACCTCGGGCAC | MAB_4098c | 7,746 | 558 | − |
| ATGCCGCTGATGTACACGGTGGACCT | MAB_4098c | 7,746 | 3,030 | − |
| CATCTATGGGCATACGCCGTTGCTCA | MAB_4098c | 7,746 | 5,057 | − |
| CGCTCGACGACGTACGGTGAGCTCGA | MAB_4098c | 7,746 | 4,734 | − |
| AAACTCAAGCCATACACCTACATTTC | MAB_4098c | 7,746 | 6,930 | − |
| CGCTGACACCCATACAAGAAGGTCTG | MAB_4098c | 7,746 | 3,316 | − |
| CCGTTGCCGATCTACTGGATCAGTTG | MAB_4099c | 10,365 | 5,458 | − |
| ACCATGGACACCTATGCCAGTGCCGG | MAB_4099c | 10,365 | 8,286 | − |
| CGCGTGGACAATTAGATATCTGGCTT | MAB_4099c | 10,365 | 46 | − |
| CGTCTTCCTCCCTATATGGTTCCGGC | MAB_4099c | 10,365 | 2,748 | − |
| CCAAGCCAGGCGTATGGCCGTTGTGC | MAB_4099c | 10,365 | 1,933 | − |
| AACAGGGACTGCTATTCCAGGCCGGA | MAB_4099c | 10,365 | 4,582 | − |
| GTTGGAGAGTTGTATGTGGCCGGCTC | MAB_4099c | 10,365 | 6,936 | − |
| GCCTGCCTGGAGTACATCGAACAAAA | MAB_4100c | 231 | 150 | − |
| GCCGAGCTGCACTACCTGTTCGAGAA | MAB_4163c | 1,647 | 279 | − |
| ACGAGGGGTACGTACAGGTCGCCGAG | MAB_4207 | 1,350 | 316 | + |
| AACATCCTCTACTACATGGTGGTCAC | MAB_4485 | 1,347 | 795 | + |
| GAATCCCGTTGCTATGTACCTGGTTC | MAB_4,554c | 1,485 | 680 | − |
| CTGCTGCGGTTGTACTGGCGGCGGTG | MAB_4573c | 918 | 537 | − |
| GCCGGCGAGCAGTATCTCGACTTCCG | MAB_4594c | 1,359 | 387 | − |
| GGTGCGCGCATGTATCGCACCGGTGA | MAB_4691c | 24,327 | 21,003 | − |
| GCTTGGGTTGGTTATGTGTTCCTCAT | MAB_4803 | 378 | 135 | + |
| TGGGGCGTGCTCTACTACGCCTTCCC | MAB_4839c | 1,194 | 102 | − |
| CGACACGGTCATTACGGTGGGCAAGC | MAB_4867c | 1,965 | 371 | − |
| GCTTAGCCGAACTACCTTCGGCACTG | MAB_4891c | 2,541 | 1,609 | − |
a
The identified genes are candidates to be involved in an S→R colony morphology transition. The TA hits are indicated in bold.