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. Author manuscript; available in PMC: 2020 Oct 21.
Published in final edited form as: AIDS. 2013 Jul 31;27(12):1867–1877. doi: 10.1097/QAD.0b013e3283618487

Fig. 2. Expression of Eomes and CD127 in CD8 T-cell subsets from healthy controls and HIV-infected patients.

Fig. 2.

(a) The analysis of mRNA expression of the transcription factor EOMES and the effector molecules granzyme B (GZMB) and perforin (PRF1) were measured as described in Fig. 1. (b) The protein expression of Eomes and CD127 in CD8 T-cell subsets were analyzed by flow cytometry in PBMCs from healthy controls (n = 13), HIV-infected patients with viral load less than 50 copies/ml (n = 13) and HIV-infected patients with viral load more than 50 copies/ml (n = 14). Median fluorescence intensities for CD127 and Eomes were calculated using FlowJo. A representative example from each of the three cohorts is displayed (gating strategy is shown in Figure S2). (c) Comparisons of Eomes expression in CD8 T-cell subsets between HIV-infected groups and healthy controls. (d) Correlations between Eomes expression and HIV-RNA levels and (e) Correlations between CD127 and Eomes expression.