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. Author manuscript; available in PMC: 2020 Oct 21.
Published in final edited form as: Nature. 2020 Jan 29;578(7793):166–171. doi: 10.1038/s41586-020-1952-2

Extended Data Figure 8. GPC3 promotes gliomagenesis and synaptic imbalance.

Extended Data Figure 8.

a) Immunohistofluorescence analyses of 3xCr control and GPC3 overexpression tumors for BrdU, excitatory synapses, and inhibitory synapses. White scale bar = 50μm; blue scale bar = 5μm. b) Quantification of immunohistofluorescence analysis. NBrdU = 4 mice of each condition; NtechnicalBrdU-3xCr = 18; NtechnicalBrdU-GPC3 = 16. Nsynapse = 3 mice of each condition. Ntechnical-synapse = 15 for each condition and synapse type. Field for BrdU incorporation = 1600μm2 field; field for synapse analysis = 34,000μm2. Plots are centered on mean, boxing the interquartile range (25th-75th percentiles), with whiskers extending to 1.5 times the interquartile range above and below. p-values:* p<.05; ** p<.01; *** p<.001; calculated by One-way ANOVA.