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. 2020 Oct 20;6(10):e05302. doi: 10.1016/j.heliyon.2020.e05302

Table 4.

The levels and factors of Box-Behnken design.

Protein Code Factor Level
-1 0 1
Chicken liver 1 A pH 6 6.5 7
B S/L 3:1 2:1 1:1
C t (h) 2 3 4
L. edodes2 A T (°C) 45 50 55
B E/S (%) 0.2 0.4 0.6
C t (h) 2 3 4
T. molitor3 A t (h) 3 4 5
B S/L 1:1 1:3 1:5
C pH 8 8.5 9
D E/S (%) 0.8 1.2 1.6
1

The level of other insignificant factors based on the results of single-factor experiments (Figure 5), choosing the level of highest DH, which were T = 55 °C, E/S = 0.6 %.

2

pH = 5, E/S = 1:8.

3

T = 55 °C.