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. Author manuscript; available in PMC: 2021 Feb 5.
Published in final edited form as: Nature. 2020 Aug 5;586(7830):567–571. doi: 10.1038/s41586-020-2622-0

Extended Data Figure 6. A single dose of mRNA-1273 elicits robust antibody responses.

Extended Data Figure 6.

BALB/cJ mice (n=10/group) were immunized with 0.01 (green), 0.1 (blue), 1 μg (red), or 10 μg (purple) of mRNA-1273. Sera were collected 2 (unfilled circles) and 4 (filled circles) weeks post-immunization and assessed for SARS-CoV-2 S-specific total IgG by ELISA (a) and neutralizing antibodies against homotypic SARS-CoV-2 pseudovirus (b). (c) S-specific IgG2a and IgG1 were also measured by ELISA, and IgG2a to IgG1 subclass ratios were calculated. (a-b) Timepoints were compared within each dose level by two-sided Wilcoxon signed-rank test, and doses were compared 4 weeks post-boost by Kruskal-Wallis ANOVA with Dunn’s multiple comparisons test. * = p-value < 0.05, ** = p-value < 0.01, *** = p-value < 0.001, **** = p-value < 0.0001. (c) Doses were compared by two-sided Mann-Whitney test, and no significance was found. Data are presented as GMT +/− geometric SD (a-b) or mean +/− SD (c), and dotted lines represent assay limits of detection.