Correction of “Zhao, Z., B. Ye, D. Yue, P. Li, B. Zhang, L. Wang, and Q. Fan. 2020. Construction of a Baculovirus Derivative to Produce Linearized Antheraea pernyi (Lepidoptera: Saturniidae) Multicapsid Nucleopolyhedrovirus Genomic DNA. J. Insect Sci.”
In the original article, the primer sequence for egfp F in Table 1 was incorrectly entered. The correct sequence is GTGAACCGCATCGAGCTGAA. This has been corrected in Table 1. The authors regret the error.
Table 1.
Primers used for the construction of the baculovirus transfer vectors and qPCR in this study.
| Primer name | Primer Sequences (5’–3′) | purpose |
|---|---|---|
| PapHindIII-F | GGAAGCTTGCGCAAGAACGAGTCGTACTTG | PCR |
| PapBamHI-R | CCGGATCCTTATAGGAAATTTTACTACAAAG | PCR |
| PapKpnI-AvrII-F | ACGGTACCTAGGAAACTTATTGTCAACTGGAGCGG | PCR |
| PapEcoRI-R | CCGAATTCTAAGCACAAAAACGGCTCCC | PCR |
| Pegfp-BamHI | CCGGATCCATGGTGAGCAAGGGCGAGGAGC | PCR |
| Pegfp-KpnI | CCGGTACCTTACTTGTACAGCTCGTCCATG | PCR |
| Pap-seqF | TTAACGCTTAGCCAGCAGCAG | PCR |
| Pap-seqR | TTCTTTACCGCTCCAGTTGAC | PCR |
| egfp | F: GTGAACCGCATCGAGCTGAA R: GACGTTGTGGCTGTTGTAGTTG |
qPCR |
| tpo | F: CTCTTGAATGGAACTCGTGG R: CAGGGAGCCTGTGTCTGATG |
qPCR |
| DNApol | F: CTCCGCTATAGAACACGTGCT R: GTACATGAGCATGTCCGACC |
qPCR |
Relevant restriction sites incorporated in the primers are italicized.