Figure 4. Addition of rhTRAIL does not convert the response to the compound in normal fibroblasts from anti-proliferative to apoptotic.

(A) HFF cells were treated with a vehicle control or increasing concentrations of ONC201 for 72 hours. rhTRAIL was added for 4 hours and cell viability determined using CellTiterGlo reagent. Luminescent images of the 96 well plates are shown. (B) Dose-response curves generated from the quantitation of the data in (A). (C) HFF and MDA-MB-361 cells were treated as above and the induction of cell death was assessed using western blot. (D) HFF and ZR751 cells were treated as above and stained with propidium iodide. Flow cytometric analysis of the cells was used to determine the percentage of cells with subG₁ DNA content. Experiments shown in this figure were conducted in triplicate.