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(A) ROS (DCFH-DA) and DAPI stain are shown for PolyHb and a vehicle control. (B) ROS (DCFH-DA) fluorescent intensity of digested A549 cells after 24 hour exposure to PolyHb and a vehicle control. (C) Effects of PolyHb and a vehicle control on expression of HIF-1α, p53, and actin, as assessed by western blot.
