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Mobile phase gradient for the analytical High-Performance Liquid Chromatography (HPLC).
| Time (min) | Mobile Phase Proportion (%) | |
|---|---|---|
| Solvent A | Solvent B | |
| 0.0 | 0.0 | 100 |
| 1.0 | 3.0 | 97.0 |
| 45.0 | 40.0 | 60.0 |
| 55.0 | 40.0 | 60.0 |
| 56.0 | 60.0 | 40.0 |
| 66.0 | 60.0 | 40.0 |
| 67.0 | 0.0 | 100 |
| 82.0 | 0.0 | 100 |
