FIG 2.
Recruitment of soluble fibronectin to Embp- or FnBPA-expressing S. epidermidis. S. epidermidis 1585Δembp, 1585Δembp × pFNBA4, and 1585Pxyl/tetembp were incubated with fibronectin. After washing, cell surface-localized Fn was detected using rabbit anti-Fn IgG and Alexa 488-conjugated anti-rabbit IgG. Bacteria were stained with DAPI (white) and Fn (green). While S. epidermidis 1585Δembp × pFNBA4 is able to recruit soluble Fn to the cell surface indicated by green fluorescence signal (C), no Fn is detected on the surface of Embp expressing 1585Pxyl/tetembp (A); S. epidermidis 1585Δembp served as a negative control (B). Scale bar, 5 μm.