TABLE 3.
Plasmids used in this study
| Plasmid | Features | Source or reference |
|---|---|---|
| pET302/NT-His_TEV-rF-repeat | Recombinant Embp fragment corresponding to aa 2569 to 2738 | This study |
| pDEST17-rFG-repeat | Recombinant Embp fragment corresponding to aa 6777 to 6902 | This study |
| pDEST17-2xrF-repeat | Recombinant Embp fragment corresponding to aa 2569 to 2908 | This study |
| pDEST17-2xrFG-repeat | Recombinant Embp fragment corresponding to aa 6777 to 7028 | This study |
| pDEST17-4xrF-repeat | Recombinant Embp fragment corresponding to aa 2569 to 3248 | This study |
| pDEST17-5xrFG-repeat | Recombinant Embp fragment corresponding to aa 6777 to 7406 | This study |
| pKOR1 | Temperature-sensitive shuttle vector for allele replacement; Cmr | 51 |
| pKO_embp | Generation of markerless deletion of embp | This study |
| pCR4-mod | pCR4 carrying a modified multiple cloning site (5′-CACCATACCCGGGATAAACGCTAGCATCGGTACGCGTAATGCACTCGAGATAAATGGTACCAAC-3′) | This study |
| pCN57 | Shuttle vector carrying gfp; Ermr | 54 |
| pCN50 | Used as a template to amplify cat194 | 54 |
| pALC2073 | Used as a template to amplify Pxyl/TetO | 55 |
| pHB2C | Derivative of pCN57; carries cat194 and Pxyl/TetO | This study |
| pEmbp_5F | In trans expression of aa 2636 to 3693 | This study |
| pEmbp_9FG | In trans expression of aa 4506 to 5693 | This study |
| FN-YPet/pHLSec2 | Wild-type Fn cDNA corresponding to RefSeq NP_997647 and harboring additional mYFP inserted between FN6 and FN7 | 28 |
| FNΔIII11-14/pHLSec2 | Fn cDNA corresponding to RefSeq NP_997647 and carrying additional mYFP following FN10 and a deletion of FN11 to FN14 | 28 |