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. 2020 Oct 16;25(20):4752. doi: 10.3390/molecules25204752

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Heatmap and relative expression levels of the selected durum wheat genes. The relative expression levels are referred to the different compounds’ pre-treatments (Chitosan C, Tebuconazole T, Azoxystrobin A, Tebuconazole + Azoxystrobin T + A) applied on the flag leaf and to the two wheat genotypes Marco Aurelio and DBC480. Fg represents the F. graminearum inoculated control. Relative expression values were obtained by using the equation 2^−ΔΔCq with TaFNR, TaTUB, and TaACT as reference genes and mock treatment used to normalize the relative expression levels. The heatmap was constructed with the z-score by analyzing data with ClustVis software (Tartu, Estonia).