Figure 8.

Effects of the administration of AST and injection of ISO on the content of the mitochondrial protein 2′, 3′-cyclic nucleotide 3′-phosphodiesterase (CNPase), subunit b, and subunit c in rat heart mitochondria isolated from rats of each group under mPTP opening. (a) Western blots stained with the corresponding antibodies under control and threshold [Ca²⁺] conditions; (b) diagrams quantitatively reflecting changes in the CNPase content in absolute units normalized to Tom20; (c) diagrams quantitatively reflecting changes in the level of subunit b in absolute units normalized to Tom20; (d) diagrams quantitatively reflecting changes in the level of subunit c in absolute units normalized to Tom20. The data are presented as the means ± SDs of three independent experiments. * p < 0.05 indicates a significant difference in the protein level relative to the control (group 1). # p < 0.05 compared with RHM isolated from ISO-injected rats (group 3). The statistical significance of the differences between the pairs of mean values was evaluated using an ANOVA type 2 (Student–Newman–Keuls) test.