Figure 6.

Outcomes of X1 infection compared to WT in hSTAT2 KI mice. Male and female hSTAT2 KI mice 5–7 weeks of age were infected IP with 1x10⁴ FFU of X1, WT ZIKV Clone, or HBSS as a mock infection. (A) Mouse weight was monitored during acute infection and shown here as the percent of weight change relative to the baseline set at 0 DPI. The dotted line symbolizes 0% weight change with no significant differences detected via two-way ANOVA. (B) Serum was collected via cardiac stick at 20 DPI to detect a ZIKV-reactive antibody response. ZIKV-reactive IgG was detected by indirect ELISA using ZIKV virions as antigen and donkey α mouse IgG HRP conjugate as detecting antibody. Data shown as optical density (OD) at various serum dilutions, normalized with mock infected mouse sera. (C) Serum from 20 DPI was also used to detect neutralization of ZIKV via FRNT. Results are shown here as the change in percent infectivity of ZIKV compared to untreated ZIKV. All data are representative of six replicates across two independent experiments, error bars indicate the standard error of the mean. (n = 6, **** p < 0.0001, ** p = 0.002 via two-way ANOVA).