COVID‐19‐related campus closures necessitated invention of an intensive online learning experience that introduced students to Structural Bioinformatics. The program engaged 33 students from 11 different institutions ranging from high school to graduate school. The objectives of the intensive, 1‐week Boot Camp focused on helping students build a foundation for studying the SARS‐CoV‐2 Main Protease (Nsp5) to understand how the protein evolved during the first 6 months of the COVID‐19 pandemic by exploring amino acid sequence and 3D atomic‐level structure using various structural bioinformatics tools. Participants analyzed how the protein changed as the virus spread around the world, comparing Nsp5 from the original viral isolate to 161 unique sequence/structure variants.
Hosted by the RCSB Protein Data Bank (RCSB.org) 1 , 2 and the Rutgers University Institute for Quantitative Biomedicine (IQB; iqb.rutgers.edu), the course met daily for 5 days, utilizing local expertise to present topics related to COVID‐19 function and evolution (e.g., evolution of RNA viruses), introductions to tools (e.g., Clustal Omega 3 for sequence alignments and phylogenetic trees; Mol* 4 for 3D molecular visualization; and Foldit 5 for structural/energetic effects of sequence mutations), and related team activities. The Boot Camp also included lectures about the COVID‐19 pandemic, SARS‐CoV‐2 protein structures (notably Nsp5 and the Spike protein), and the Rutgers RUCDR saliva test for detecting COVID‐19. After 5 days in close collaboration, 10 student/mentor teams presented their findings on the energetic effects of the 161 mutations on the plasticity of the Nsp5 3D structure.
The camp was designed and directed by members from the IQB and the RCSB Protein Data Bank, facilitated by nine mentors from Rutgers and six mentors associated with the BASIL Consortium (https://basilbiochem.github.io/basil/index.html 6 ). Student researchers were selected from the RISE program at Rutgers (https://www.rise.rutgers.edu/), from other Rutgers‐affiliated programs, and from the campuses of the BASIL mentors. Most students were Chemistry/Biochemistry majors without prior programming experience.
Daily sessions ran approximately 6 h, including breaks and lunch. Most active learning took place in virtual breakout rooms where teams worked through data analysis exercises with their mentors. Progress vs. goals was reviewed during 30‐min faculty/mentor meetings at the close of each day.
The major challenge in delivering an online experience was monitoring student engagement. Zoom video conferencing breakout rooms were key to providing space for students and mentors to interact with the material in small groups. A parallel Slack communication channel was also helpful in communicating across groups. Other lessons learned included the need for enforced break times and abbreviated lecture formats.
Overall student learning will be assessed by the faculty and mentors using student deliverables (reports and presentations) and an exit survey. In addition to an increased understanding of COVID‐19, initial survey responses indicate that most participants felt they had increased their computational proficiency (FoldIt) and their knowledge of structural biology (protein secondary structure, molecular energetics, molecular modeling, and the Protein Data Bank).
Select materials for replicating Boot Camp modules are available online for reuse at https://covid19-bootcamp.rcsb.org.
ACKNOWLEDGMENTS
We thank the Boot Camp students for their willingness to join us on this adventure, and Ken Dalenberg for technical expertise.
RCSB PDB is funded by the National Science Foundation (DBI‐1832184), the US Department of Energy (DE‐SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.
BASIL is funded by the National Science Foundation IUSE 1709170, 1709355, 1709805, 1709278.
Joseph Lubin is funded by the Rutgers NIH Biotechnology Training Program, NIH T32 GM008339.
Burley SK, Bromberg Y, Craig P, et al. Virtual Boot Camp: COVID‐19 evolution and structural biology. Biochem Mol Biol Educ. 2020;48:511–513. 10.1002/bmb.21428
Funding information National Institutes of Health, Grant/Award Numbers: R01GM133198, T32 GM008339; National Science Foundation, Grant/Award Numbers: DBI‐1832184, IUSE 1709170, 1709355, 1709805, 1709278; US Department of Energy, Grant/Award Number: DE‐SC0019749
Contributor Information
Michael J. Pikaart, Email: pikaart@hope.edu.
Christine Zardecki, Email: christine.zardecki@rcsb.org.
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