Figure 3.

ATG5 and ATG7 mediate pexophagy induced by depletion of HSPA9. (A and B) HeLa cells (WT) and ATG5 and ATG7 knockout HeLa cells (ATG5 KO and ATG7 KO, respectively) expressing turquoise-Peroxi (green) were transfected with scrambled siRNA (Sc) or HSPA9-targeting siRNA (siHSPA9). (A) After 5 d, the cells were stained with DRAQ5 (red) and imaged. (B) Cells with a reduced number of peroxisomes were counted. (C and D) WT, ATG5 KO, and ATG7 KO HeLa cells were transfected with scrambled siRNA (Sc) or HSPA9-targeting siRNA (siHSPA9). After 5 d, the cells were harvested and analyzed by western blotting using the indicated antibodies. Data are presented as the mean ± SEM (n = 3, * p < 0.05). Scale bar: 10 µm