Figure 7.

HSPA9 mutants fail to rescue pexophagy in HSPA9-depleted neuroblastoma cells. (A-C) SH-SY5Y cells were transiently co-transfected with siRNA-resistant HSPA9 WT or HSPA9 mutants together with HSPA9-targeting siRNA (siHSPA9). (A) Protein expression of HSPA9 was analyzed by western blotting. (B) Cells were further stained with anti-ABCD3 antibody (red) and imaged by confocal microscopy. (C) Cells exhibiting pexophagy were counted. Data are presented as the mean ± SEM (n = 3, * p < 0.05). Scale bar: 5 µm