Figure S3.
HSC and Progenitor Kinetics following Aerosolized Mtb Infection, Related to Figure 3
(A-M) WT mice were infected with aerosolized Mtb. (A) Day 1 lung CFU following aerosol infection. (B-H) Kinetics of the frequencies among BM cells of LT-HSC (B), ST-HSC (C), MPP (D), MPP3/MPP4 (E), CMP (F), GMP (G), CLP (H) in Mtb infected WT mice. (I) Total cell counts of cMoPs in the BM of WT mice at 120 days post-infection. Kinetics of the frequencies and total numbers of neutrophils and Ly6Chi monocytes in the BM (J-K) and lung (L-M) as well as the frequencies and total cell counts of adaptive CD8 and CD4 T cells in the lung (N). (O) Average expression of cell-type markers across clusters. (P) Correlations between genome-wide expression patterns of our myeloid progenitor data and results published by Paul et al. (2015). In each column, Spearman correlations are normalized to cover the range [0-1]. Blue boxes mark the best fit (i.e., candidate identity match) for each of our clusters. (Q) Expression patterns across clusters for some marker genes associated to commitment to the different lineages characterized in this study. (R) Proportion of cells per cluster in both sub-populations (LKS and myeloid progenitors), for cells coming from PBS versus Mtb treated mice. (S) Fisher exact test enrichments (log2 odds ratios) for the fraction of Mtb cells in each cluster. (T) Number of PBS versus Mtb cells in each cluster. (U) Genome wide correlation of Mtb effect sizes (logFC) across clusters. Statistics were (B-H, J-N) One-way ANOVA followed by Tukey’s Multiple Comparisons Test with significance shown compared to day 0 or day 1 post-infection mice for each cell type and Two-tailed Student’s t test (I).