Figure S5.

The IFN-I/Iron Axis Regulates Cell Death of Myeloid Progenitors, Related to Figure 5

Frequency of necrotic (NucSpot+ AnnexinV^-) CLP (A) in the BM of BCG-iv and Mtb-iv infected mice at day 7. (B-D) Frequency among single viable BM cells (left panels) and total cell counts (right panels) of CMP (B), GMP (C), CLP (D) of naive WT and Ripk3^−/− mice. (E) Frequency among BM cells of CLP in WT and Ripk3^−/−Mtb-iv infected mice at day 7. (F) Frequency of CLP with disrupted mitochondria in the BM of BCG-iv and Mtb-iv infected mice at day 7. (G) Relative levels of mitochondrial iron (Fe²⁺) in CLP of BCG-iv and Mtb-iv infected mice at day 7. (H) Representative histogram of expression of CD71 (left panel) and quantification of CD71 expression (right panel) on CLPs in the BM of Mtb-iv infected WT and Ifnar1^−/− mice at day 28. (I) Expression of CD71 on CLPs in the BM of WT and Poly (I:C)-treated WT and Ifnar1^−/− mice. (J) Relative proportion of mitochondrial iron dye (Fe²⁺) in the CLP of Poly (I:C)-treated WT and Ifnar1^−/− mice. Frequency of CLPs with a disrupted mitochondrial potential (K), high mitochondrial ROS (L), or those that are necrotic (M). Differences were assessed via One-way ANOVA followed by Tukey’s Multiple Comparison Test (A; F-G), Two-tailed Student’s T-Test (B-E), Two-way ANOVA followed by Sidak’s Multiple Comparisons Test (H-M).