Figure 3.

Expression of P23H Flag-tagged rhodopsin in adult zebrafish retina. (A,B) The Flag-tagged P23H mutant rhodopsin (red) is expressed by sparsely-distributed photoreceptors in the adult fish (B); there is no labeling in wild type fish (A). (C,D) Mutant rhodopsin (red) colocalizes with rhodopsin (Retp1, green) in the rods. (E,F) Mutant rhodopsin (red) is expressed only by the rods and not the cones, as seen with the cone marker Zpr1 (green). Yellow arrowheads represent the cone axons and yellow arrows represent the cone myoids. ONL: outer nuclear layer; INL: inner nuclear layer; OS: outer segments. Scale bar in B applies to all panels.