Table 1.
Rat oligonucleotide primers for detection of mRNA and Hp oligonucleotide primer for detection of 16S rRNA by RT-PCR and PCR, annealing temperature and size of PCR products employed in the experimental protocol.
| Gene | Primer Sequence | Size of PCR Product (bp) | Annealing Temp. (°C) |
|---|---|---|---|
| 18S | Forward 5′-GTTGGTTTTGATCTGATAAATGC-3′ Reverse 5′-CATTAAATCAGTTATGGTTCCTTTG-3′ |
143 | 60 |
| Bax | Forward 5′-CTGCCAACCCACCCTGGTCT-3′ Reverse 5′-TGGCAGCTGACATGTTTTCTG-3′ |
195 | 55 |
| BCL2 | Forward 5′-ACTGAGTACCTGAACCGGCATC-3′ Reverse 5′-GGAGAAATCAAACAGAGGTCGC-3′ |
108 | 60 |
| c-Myc | Forward 5′-CCACACAGCCCACTGGTCCT-3′ Reverse 5′-GGCTGGAGCATTTGCGGTTGTT-3′ |
163 | 60 |
| Cyclin D1 | Forward 5′-TGCTTGGGAAGTTGTGTTGG-3′ Reverse 5′-AATGCCATCACGGTCCCTAC-3′ |
126 | 60 |
| c-Myc | Forward 5′-CCACACAGCCCACTGGTCCT-3′ Reverse 5′-GGCTGGAGCATTTGCGGTTGTT-3′ |
163 | 60 |
| Ki-67 | Forward 5′-AACCAGGACTTTGTGCTCTGTAA-3′ Reverse 5′-CTCTTTTGGCTTCCATTTCTTC-3′ |
209 | 60 |
| Klf4 | Forward 5′-TTCTCCACGTTCGCGTCCGG-3′ Reverse 5′-TCTCGCCAACGGTTAGTCGGGG-3′ |
80 | 60 |
| Oct4 | Forward 5′-GGAGGGATGGCATACTGTGGACCT-3′ Reverse 5′-TCCTGGGACTCCTCGGGACTAGG-3′ |
197 | 60 |
| p53 | Forward 5′-AGTGAAGGGACTAGCATTGTC-3′ Reverse 5′-GGATGCCCGTGCTGCCGAGGAG-3′ |
243 | 60 |
| Sox2 | Forward 5′-AGAACCCCAAGATGCACAAC-3′ Reverse 5′-CTCCGGGAAGCGTGTACTTA-3′ |
204 | 60 |
| TGFβ rec 2 | Forward 5′-TGTGGCAGAGCGCTTCAGT-3′ Reverse 5′-TGTTCAGGGAGCCGTCTTCT-3′ |
95 | 60 |
| TGFβ rec 1 | Forward 5′-GCAGACTGGACCAGCAATGAC-3′ Reverse 5′-CTGCAATCAGGATCACTGCAA-3′ |
118 | 60 |
| Hp 16S | Forward 5′-GTCAAGAGATCAGCCTATGTCC-3′ Reverse 5′-TGGCAATCAGCGTCAGGTAATG-3′ |
522 | 54 |