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. 2020 Oct 7;9(10):820. doi: 10.3390/pathogens9100820

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Obtained PCR amplicons in this study. Midori Green stained agarose gel showing the size variation of the ITS (ITS1, 5.8S rDNA and ITS2) PCR product obtained from Naegleria isolates. Lane 1: 100 bp DNA ladder. Lane 2: PCR reaction without DNA template as negative control. Lane 3: N. fowleri ATCC 30808 and Lane 4 N. gruberi ATCC 30540 as positive controls. Lane 5 to Lane 15 correspond to the isolates: 5: C4abrilENL; 6: CRENL; 7: CRES1; 8: CRES4a; 9: CRES3a; 10: CRES4b; 11: CRET1; 12: CRET2; 13: CRES2; 14: CRES3b and 15: CDENL. DNA amplicons separated by 2.5% agarose gel electrophoresis.