Table 1.

Therapeutic miRNA-enriched EVs: Evidence from Disease models.

	References	Therapeutic miRNA	Enrichment/Sorting Method of miRNAin EVs	Source of EVs	Disease Model	Mode of Action
1.	[49]	miR-126	Transfection of miR-126 mimic	Adipocyte-derived stem cells	Myocardial Infarction	Decrease in cardiac apoptosis and fibrosis
2.	[53,54]	miR-182-5p	Transfection with miR-182-5p	Mesenchymal Stem cells	Ischemic injury	Reduced apoptosis in cardiomyocytes
3.	[52]	miR-223	miR-223 KO	Mesenchymal Stem cells	Cardiac injury (sepsis)	Decreased inflammation and cell death
4.	[55]	miR-125-b	-	Mesenchymal Stem cells	Myocardial infarction	Reduced autophagy flux and cell death
5.	[51]	miR-21	Over-expression of miR-21	HEK-293T	Myocardial Infarction	Antiapoptotic effect by targeting PDCD4 in cardiomyoblasts
6.	[50]	miR-210	Over-expression of miR-210	Mesenchymal stem cells	Myocardial infarction	Initiation of angiogenesis by targeting EFna-3 in endothelial cells
7.	[40]	miR-1, miR-302-367	Over-expression of respective miRNAs	Patient-specific glioblastoma stem cells	Glioblastomas	Reduced Growth and invasiveness in xenografts
8.	[56]	miR-133	Transfection of MSCs with miR-133 mimic	Mesenchymal Stem cells	Intracerebral hemorrhage	Decrease in the number of neurodegenerative neurons
9.	[45]	miR-30d	Transfection of miR-30d	Adipose derived stem cells	Acute Ischemic Stroke	Microglial/Macrophage polarization and suppression of autophagy
10.	[48]	miR-26-5p	-	Astrocytes	-	Dendritic Arborization in hippocampus neurons
11.	[57]	miR-146a	Transfection with miR-146a mimic	Dendritic cells	Myasthenia Gravis	Altered T helper cells from Th1/Th17 to Th2/Treg
12.	[58]	miR-124-3p	Transfection with miR-124	Microglial	Traumatic brain injury	Inhibit neural autophagy
13.	[59]	miR-133	Transfection with miR-133 mimic	Mesenchymal Stem cells	Spinal cord injury	Regeneration of axons, preservation of neurons
14.	[60]	miR-124	Stable over-expression of the respective miRNA	HEK-293 cells	Huntington Disease	Reduction in RE1-Silencing Transcription Factor but no significant difference in behavior
15.	[46]	miR-133b	Knock-in and knock-down in miR-133b	Mesenchymal Stem cells	Stroke	Neurite Remodeling and post-stroke functional recovery
16.	[61]	miR-146-b	Transfection of the respective miRNA via electroporation	Mesenchymal Stem cells	Glioma	Reduced growth
17.	[62]	Anti-miR-142-3p	Transfection of the respective anti-miRNA via electroporation	Mesenchymal Stem cells	Breast Cancer	Reduced growth and metastasis
18.	[63]	miR-128-3p	Electroporation in exosomes	Dendritic cells	Colon Cancer	Increased Chemosensitivity
19.	[64]	miR-451a	-	Tumor-associated stromal cells	Pancreatic cancer	Promotion of apoptosis and reduced proliferation
20.	[65]	miR-302a	Over-expression of miRNA	Human Umbilical cord mesenchymal stem cells	Endometrial cancer	Reduced Proliferation and migration by targeting cyclin-D1
21.	[66]	miR-159	Incubation at 37 °C	Human Monocyte macrophage cells	Triple Negative Breast Cancer	Anti-cancer effect by targeting TCF gene
22.	[67]	miR-335	Transfection of exosomes	LX2 cells	Hepatocellular carcinoma	Less proliferation and enhanced apoptosis in hepatic tumors
23.	[68]	miR-181-5p	Over-expression of miRNA	Mesenchymal stem cells	Fibrosis	Reduces autophagy and inflammation by targeting and downregulating STAT-3 and BCl-2
24.	[69]	miR-185	Over-expression of miRNA	Mesenchymal stem cells	Oral Leukoplakia	Promote apoptosis by specifically targeting Akt genes
25.	[70]	miR-92a-3p	Over-expression of miRNA	Mesenchymal stem cells	Chondrogenesis (can be used in osteoarthritis)	Cartilage proliferation
26.	[71]	miR-375	Over-expression of miRNA	Mesenchymal stem cells	Rat model of calvarial defect	Bone regeneration
27.	[72,73]	miR-223	Over-expression of miRNA	Bone marrow derived stem cells	Experimental autoimmune hepatitis	Protection from liver injury
28.	[74]	miR-140	Over-expression of miRNA	Human Synovial stem cells	Osteoarthritis	Prevention of osteoarthritis by increasing proliferation and migration of chondrocytes