Fig. 3.

Antibody half-life is differentially impacted by isotype and multimerization state. Normalized T50% for each mAb was determined as the mean time in minutes for a 50% reduction in immunoreactivity relative to a positive control. Antibodies that displayed >50% immunoreactivity over the course of the assay were assigned a T50% of 120 min for purposes of analysis and are shown on the graphs for clarity. Significance across mAbs for a given construct is shown at the top of each graph. Due to the small sample size for mAb3 dIgA2 (N = 2), dIgA2 was not analyzed.