Fig. 7.

(A) Top: Confocal images of motoneurons (MNs) immunolabeled with antibodies against Ac-H3K9, Ac-p53 (k373), Ac-p300 (k1542), and p300 in red and counterstained with FluoroNissl (green) and DAPI (blue) from MNs of wild-type (WT) and SIRT2 KO animals at 30 dpi. Scale bar = 100 μm and 20 μm. Down: Bar graphs of the mean of the Ac-H3k9, Ac-p53, and the ratio of Ac-p300/total p300 immunofluorescence intensity inside the cytoplasm or nuclei from injured MNs (n = 4, t test, *P < 0.05 vs. WT). (B) Representative microphotographs and bar graphs showing the mean (± SEM) values of neurite elongation and maximum neurite length per cell from SH-SY5Y cells after 24 h of treatment with vehicle (Veh), DMOG, AK7, C646, or AK7+C646, or eGFP, eGFP+AK7, shRNA/p300, and shRNA/p300+AK7. Insets are a black and white magnification of a single cell at each condition (n = 3–4, ANOVA, post hoc Bonferroni *P < 0.05 vs. Veh. Scale bar = 100 μm; inset, 20 = μm). (C) Western blots and histogram showing the analysis of p53, Ac-p53, and GAP43, in different experimental groups (n = 3–4, ANOVA, post hoc Bonferroni, *P < 0.05 vs. Veh, ^#P < 0.05 vs. AK7)