Fig. 2.

(A) Fever burden in TLR4^fl/fl SAH mice was compared with the fever burden of global TLR4^−/− SAH mice. The lack of TLR4 completely inhibited the SAHiP response. Statistical significance was determined by student’s t test (p < 0.0001; n = 3 F, n = 3 M). (B) Prostaglandin pathway involvement in SAHiP was determined by performing SAH in Nestin^Cre: ptger3^fl/fl mice, which yielded the same fever burden as observed for TLR4^fl/fl SAH and ptger3^fl/fl + 100 μg meloxicam SAH mice. The SAHiP response was unaffected by the total lack of prostaglandin EP3 receptors in neurons, and the inhibition of the cyclo-oxygenase enzyme by meloxicam. Statistical significance was determined by two-way ANOVA and showed no differences (n = 6 per group, equally divided between the genders)