Fig. 4.

Determination of whether microglial TLR4, neutrophil TLR4, or both were necessary for SAHiP. SAH was induced in global TLR4^−/−, microglial TLR4^−/− (CX₃Cr1^CreER:TLR4^fl/fl mice), and mice that lacked TLR4 on microglia and all peripheral myeloid cells (LyzM^Cre:TLR4^fl/fl mice). The fever burden in all murine subsets were determined to be the same and indicated that only microglial TLR4 was necessary to mediate SAHiP. Statistical significance was determined by ANOVA and showed no differences (n = 6 per group, equally divided between the genders)