Glycogen synthase kinase 3β in tumorigenesis and oncotherapy

Abstract

Glycogen synthase kinase 3β (GSK 3β), a multifunctional serine and threonine kinase, plays a critical role in a variety of cellular activities, including signaling transduction, protein and glycogen metabolism, cell proliferation, cell differentiation, and apoptosis. Therefore, aberrant regulation of GSK 3β results in a broad range of human diseases, such as tumors, diabetes, inflammation and neurodegenerative diseases. Accumulating evidence has suggested that GSK 3β is correlated with tumorigenesis and progression. However, GSK 3β is controversial due to its bifacial roles of tumor suppression and activation. In addition, overexpression of GSK 3β is involved in tumor growth, whereas it contributes to the cell sensitivity to chemotherapy. However, the underlying regulatory mechanisms of GSK 3β in tumorigenesis remain obscure and require further in-depth investigation. In this review, we comprehensively summarize the roles of GSK 3β in tumorigenesis and oncotherapy, and focus on its potentials as an available target in oncotherapy.

1. Introduction

Glycogen synthase kinase 3 (GSK3), a member of the mitogen-activated protein (MAP) kinase superfamily, has two highly conserved homologous isoforms, GSK 3α (51 kDa) and GSK 3β (47 kDa). Discovered in 1980, both of these two isoforms are multifunctional serine and threonine protein kinases, and they are widely expressed in all eukaryotes (1–3). Encoded by the human chromosome 19q13.2 and 3q13.33, respectively, GSK 3α and GSK 3β share 98% identical sequences in their kinase domains (4,5). Despite structural similarities, their functions are different by phosphorylation at distinctive sites (5). For the activation of GSK 3α and GSK 3β, tyrosine 279 (Y279) and tyrosine 216 (Y216) are phosphorylated by upstream signaling molecules respectively, and these two sites are located at the T-loop of GSK 3 (activation domain) (Fig. 1). Notably, GSK 3α and GSK 3β are inactivated by site-specific phosphorylation, which is tightly controlled by diverse mechanisms (6). All these mechanisms for the inactivation of GSK 3α at Ser21 and GSK 3β at Ser9 have been revealed to be attributed to the phosphoinositide3 kinase (PI3K)-dependent mechanism (7). Activated by PI3 kinase, protein kinase A (PKA), protein kinase B (PKB) (also termed as Akt), protein kinase C (PKC) and p90Rsk contribute to the inactivation of GSK 3, which ultimately leads to the dephosphorylation of GSK 3 substrates (8). Initially, the inactivation of GSK 3 was considered to be a critical mediator in glycogen metabolism and insulin signaling, since it could give rise to the phosphorylation of glycogen synthase and could promote glycogen synthesis (9). Currently, emerging evidence has demonstrated that GSK 3, particularly GSK 3β, is well-established as a vital component in signaling pathways of cell regulation, involving cell proliferation, differentiation, motility, apoptosis and the cell cycle (1,2,10). As an attractive therapeutic target, GSK 3 has been revealed to be implicated in the pathogenesis of numerous human diseases, such as diabetes, inflammation, Alzheimer's disease, cardiovascular and bipolar disease (11–13). In addition, GSK 3β has been demonstrated to be a transcription factor regulating tumor progression, invasion and metastasis (14,15). In different types of cancers, GSK 3β plays a controversial role as a tumor suppressor or a tumor promoter (16,17). Furthermore, GSK 3β has been demonstrated to be a key regulator of chemo-resistance and radio-resistance in tumor treatment (18,19). Therefore, the regulative roles of GSK 3β in tumor development and progression require elucidation. In addition, to date, it has been revealed that GSK 3β inhibitors are potential therapeutic drugs in cancer therapy, thus, pre-clinical and clinical studies of GSK 3β inhibitors are also presented.

Figure 1.

Functional domains and tertiary structures of GSK 3β (Homo sapiens). GSK 3β is a 47-kDa protein consisting of 433 amino-acids in human. The protein contains an N-terminal domain, kinase domain and C-terminal domain. Phosphorylation at Tyrosine (216) in the N-terminal region of GSK 3β activates this kinase. Phosphorylation at Serine (9) in the N-terminal region of GSK 3β leads to the inactivation of this kinase. The BD includes GSK 3β specific binding sites for substrates and protein complexes. GSK 3β, glycogen synthase kinase 3β; BD, binding domain.

2. Regulation of GSK 3β

It has been revealed that the activity of GSK 3β could be regulated in a substrate-specific manner, which comprises four key mechanisms: Phosphorylation, subcellular localization, the formation of protein complexes and the phosphorylation status of GSK 3β substrates (12). Among them, phosphorylation is deemed to be the most important regulatory mechanism of GSK 3β, which would lead to the inactivation of GSK 3β at Ser 9. The activated PI3K/Akt signaling pathway phosphorylates GSK 3β at inhibitory serine residues and the phosphorylation may result in the activation of transcription factors in response to carcinogenesis, for instance activating protein-1 (AP-1), cyclic adenosine monophosphate (cAMP)-response element binding protein (CREB), β-catenin, c-Myc and nuclear factor-kappa (NF-κB) (1,20). In addition, upstream proteins of GSK 3β, including P70S6 kinase, extracellular signal-regulated kinases (ERKs), p90sk (also called MAPKAP kinase-1), protein kinase C (PKC) and cAMP-dependent protein kinase [protein kinase A, (PKA)], could lead to the inactivation of GSK 3β (1,21,22). Each regulatory kinase is influenced by GSK 3β distribution, while scaffolding proteins could restrain the interaction between kinases (6) (Fig. 2A). It has been established that the phosphorylation of tyrosine at 216 site of GSK 3β (Tyr216) is regulated by calcium-dependent tyrosine kinase, proline-rich tyrosine kinase 2 (PYK2), cAMP-activated protein tyrosine kinase, Zaphod kinase 1 (ZAK1) or Fyn (23,24). Additionally, phosphorylation of GSK 3β at Tyr216 is regulated by mitogen-activated protein kinase (MEK1/2) (25). Generally, GSK 3β is classified as an active constituent enzyme in the cytoplasm of resting cells. When activated, GSK 3β is accumulated in the nucleus and mitochondria (26) (Fig. 2B). Notably, by activating the genes of downstream transcription factors and recruiting the aggregation of these transcription factors, nuclear translocation of GSK 3β regulates a great number of transcription factors (27).

Figure 2.

Regulatory mechanisms of GSK 3β. The activity of GSK 3β can be inactivated by multiple pathways. PI3K/Akt, P70S6K, p90sk, ERKs and PKA/PKC can attenuate GSK 3β enzymatic activity by phosphorylating GSK 3β at Ser9. Inhibition of GSK 3β activity leads to the stabilization and accumulation of β-catenin in the cytosol. GSK 3β inactivation is also involved in glycogen synthesis, protein synthesis, cell proliferation and cell invasion. Additionally, PYK2, Ca²⁺, ZAK1 and Fyn are able to phosphorylate GSK 3β at Tyr216, which increases the GSK 3β activity. Subsequently, activated GSK 3β phosphorylates downstream target β-catenin. GSK 3β, glycogen synthase kinase3β; PI3K, phosphoinositide3 kinase; P70S6K, P70S6 kinase; ERKs, extracellular signal-regulated kinases; PKC, protein kinase C; PKA, protein kinase A; HKII, hexokinase II; AP-1, activating protein-1; MDM2, murine double minute 2; PYK2, proline-rich tyrosine kinase 2; ZAK1, Zaphod kinase 1; APC, adenomatous polyposis coli.

More than 40 proteins are downregulated by GSK 3β. These proteins are involved in a broad range of cellular processes, including protein synthesis, cell differentiation, proliferation and apoptosis (2,10,14). Substrates that are closely related to tumorigenesis and cancer development tend to be dephosphorylated. In the canonical Wnt signaling pathway, β-catenin, an important element of Wnt signaling, is controlled by GSK 3β (28). With the absence of Wnt signaling, activated GSK 3β forms a complex with adenomatous polyposis coli (APC) and the transcriptional co-activator β-catenin, and therefore the complex binds with the scaffolding protein Axin. This newly formed complex enables GSK 3β to phosphorylate β-catenin and leads to the degradation of β-catenin. Conversely, the Wnt signaling pathway could inactivate GSK 3β and prevent the phosphorylation of β-catenin by GSK 3β (29). In addition, β-catenin binds to the T-cell factor (TCF)/lymphocyte enhancer factor (LEF), and ultimately promotes the transcription of TCF/LEF transcription factor family-related genes, including proto-oncogenes, such as c-Myc, cyclin D1 and the vascular endothelial growth factor (VEGF), as well as genes that regulate cell invasion and migration, including matrix metalloproteinase-7 (MMP-7) (30–32). Therefore, as the upstream gene of β-catenin, GSK 3β plays a pivotal role in cell proliferation, migration, metastasis and differentiation by controlling Wnt signaling. Additionally, other proto-oncogenic or tumor suppressing transcription factors (such as p53) and translation factors are the substrates of GSK 3β. With regard to DNA damage studies, the increasingly activated GSK 3β in the nucleus and mitochondria promotes the expression of the tumor suppressor protein p53, and forms a complex with p53, which enhances the response of p53-induced apoptosis (33,34). Furthermore, GSK 3β directly phosphorylates Kruppel-like factor 6 (KLF6), a zinc-finger transcription factor which is a tumor suppressor, inhibiting the tumor growth by increasing KLF6-mediated growth suppression through p53-independent transactivation of p21 (35) (Fig. 3).

Figure 3.

GSK 3β regulates the key signaling proteins of the Wnt pathway. In the presence of Wnt, β-catenin is stabilized and can induce gene transcription. Wnt binds with its co-receptors Frizzled and LRP5/LRP6. Axin and APC interact with phosphorylated GSK 3β at Tyr216, which leads to β-catenin stabilization and cytosol accumulation. Subsequently, β-catenin is transferred into the nucleus where it activates the transcription of related genes by forming a complex with transcription factors as TCF/LEF. Then, TCF/LEF upregulates the proto-oncogenes (c-Myc and cyclin D1) and cell invasion/migration-related genes (MMP-7 and Cdc37). GSK 3β, glycogen synthase kinase 3β; APC, adenomatous polyposis coli; TCF, T-cell factor; LEF, lymphocyte enhancer factor; MMP-7, matrix metalloproteinase-7.

3. Role of GSK 3β in tumorigenesis

For decades, accumulating research has been exploring the role of GSK 3β in human oncology. Based on the studies of the GSK 3β signaling mechanism, the regulation of GSK 3β in cancer progression is intricate (14,36). It has been demonstrated that GSK 3β acts as a tumor suppressor in prostate cancer, by inhibiting androgen receptor-mediated cell growth (37). Conversely, it has been observed in colon cancer cell lines and colorectal cancer patients that the expression levels and the enzyme activities of GSK 3β were higher than normal cells, therefore GSK 3β appears to be a tumor promoter (38). Herein, in order to further elucidate GSK 3β as a potential therapeutic target for various cancers, a comprehensive review about the multiple roles of GSK 3β in tumorigenesis is provided.

GSK 3β as a tumor suppressor

Since GSK 3β downregulates numerous proto-oncogenic proteins and cell cycle checkpoint proteins, GSK 3β has been generally recognized as a tumor suppressor by inhibiting cell proliferation (39). Previous studies have demonstrated that PI3K inhibitor LY294002 and tumor suppressor phosphatase and tension homolog deleted on chromosome 10 (PTEN) stimulate GSK 3β-mediated degradation of β-catenin by inhibiting the PI3K/Akt pathway, thereby inhibiting the progression of prostate tumors (40,41). In addition, GSK 3β is phosphorylated and inactivated by the activation of PI3K/Akt pathway, and therefore, inactivated GSK 3β prevents the phosphorylation of cyclin D1, inhibits the expression of cyclin D1 gene (CCND1), and accumulates cyclin D1 at the nucleus of breast cancer cells (42). Moreover, it has been revealed that adiponectin could prevent Akt-induced phosphorylation of GSK 3β and decrease intracellular accumulation and nuclear activity of β-catenin, subsequently reducing the expression of cyclin D1 (43). This mechanism leads to the apoptosis of breast cancer cells MDA-MB-231 and T47D, and induces the cell cycle arrest of MDA-MB-231 cells at G0-G1 phase. Additionally, it has been revealed by in vivo experiments that the adiponectin-mediated suppressive effect on the GSK 3β/β-catenin signaling pathway could reduce mammary tumorigenesis in female nude mice (43). Therefore, GSK 3β is perceived as a tumor suppressor in the tumorigenesis and progression of breast cancer (Fig. 4).

Figure 4.

Tumor inhibitory role of GSK 3β. LY294002, adiponectin and PTEN suppress GSK 3β-participated β-catenin degradation and cell cycle arrest by inhibiting the PI3K/Akt pathway in prostate tumor and breast cancer. The deletion of MIF attenuates Akt-dependent GSK 3β phosphorylation and restores tumor suppressor activity of GSK 3β in esophageal squamous cell carcinoma. Additionally, GSK 3β phosphorylates various tumor factors, facilitates their degradation, and prevents them from entering the nucleus. GSK 3β, glycogen synthase kinase 3β; PTEN, phosphatase and tension homolog deleted on chromosome 10; AR, androgen receptor; CCND1, cyclin D1 gene; FAK, focal adhesion kinase; MMPs, matrix metalloproteinases; AP-1, activating protein-1.

Emerging evidence has revealed that GSK 3β phosphorylates various tumor factors (TFs), including cyclin D1, cyclin E, AP-1, c-Jun, p53, p65, Snail, c-Myc and β-catenin, which would facilitate their degradation and prevent them from entering the nucleus. Thereby, their activities are downregulated in oral squamous cell carcinoma (OSCC) (44–51). It has been indicated that activated GSK 3β binds to the mesenchymal Snail gene. Then it inhibits the phosphorylation, degradation and cytoplasmic translocation of Snail, and subsequently inhibits p-cadherin-induced development and homeostasis of epithelial architecture. Consequently, GSK 3β may reverse the p-cadherin-contributed oncogenesis in OSCC (52). In addition, it has been demonstrated that the overexpression of focal adhesion kinase (FAK) may activate NF-κB by inactivating GSK 3β, and therefore promotes cancer cell migration. In addition, the inactivation of GSK 3β has also been revealed to suppress cell apoptosis in pre-invasive and invasive OSCC (51,53,54). Furthermore, as the induction of intracellular reactive oxygen species (ROS) stimulates cell apoptosis (55), constitutively activated GSK 3β has been reported to increase the mitochondrial membrane potential and to promote ROS (56,57). In addition, several matrix metalloproteinases (MMPs) can also facilitate the migration of cancer cells, thereby upregulating and activating tumor factors, such as Snail, AP-1 and NF-κB in OSCC (58,59). Collectively, GSK 3β could be a potential therapeutic target for the treatment of OSCC by suppressing tumorigenesis (Fig. 4).

It has been indicated that the overexpression of macrophage migration inhibitory factor (MIF) is associated with the development and progression of esophageal squamous cell carcinoma (ESCC) (60,61). Further study has demonstrated that the deletion of MIF attenuates Akt-dependent GSK 3β phosphorylation and restores tumor suppressor activity of GSK 3β (61). After MIF knockdown, the activation of GSK 3β prevents the transcription and expression of Snail gene, one of activators of epithelial-mesenchymal transition (EMT) (62). Moreover, it has also been revealed to inhibit the expression of E-cadherin in ESCC (61). GSK 3β not only inhibits cell survival and proliferation, but also restrains tumor invasion and metastasis. GSK 3β interacts with Slug, the zinc-finger-containing transcriptional repressor, by suppressing E-cadherin expression and promoting cancer cell migration, invasion and metastasis in the non-small cell lung cancers (NSCLCs) (63). Notably, GSK 3β also reduces the stability of Slug protein by promoting the C-terminus of Hsc70-interacting protein (CHIP)-mediated Slug degradation. Conversely, GSK 3β inhibitors could induce the accumulation of non-degradable Slug, which increases the migratory and invasive capabilities of lung cancer cells (63). Collectively, the activation of GSK 3β may offer a new prospect for inhibiting the tumorigenesis and development of tumors (Fig. 4).

GSK 3β as a tumor promoter

GSK 3β also plays a critical role in tumor cell proliferation. Overexpression of GSK 3β has been observed in various tumor types, including colon, liver, ovarian and pancreatic tumors (14,64). It has been previously reported that GSK 3β inhibitors (SB-216763 and AR-A014418) significantly suppress the growth of patient-derived xenograft (PDX) colon cancer, and GSK 3β inhibitors are anticipated to be therapeutic agents for colon cancer (64). The overexpression of nuclear GSK 3β and the loss of membrane β-catenin are substantially correlated with poor survival, distant metastasis and worse prognosis of colon cancer patients (65). It is further discovered that the formation of nuclear GSK 3β and β-catenin could decrease the transcription of TCF/LEF target gene (66). In addition, it has been clarified that troglitazone, an agonist of peroxisome proliferator activated receptor γ (PPARγ), could suppress the activity of NF-κB by inhibiting the activity of GSK 3β, reducing the expression of G0/G1 phase regulatory proteins including Cdk2, Cdk4 and cyclin B1, and increasing the cleavage of apoptosis-associated proteins, such as caspase-3 and caspase-9 of human colon cancer and prostate cancer (67,68). A previous study has revealed that p53^+/+ colorectal cancer cells treated with GSK 3β inhibitor or GSK 3β silencing RNAs could facilitate the increase of p53-dependent apoptosis, instead of cell cycle arrest (69). Furthermore, the activation of PI3K/Akt signaling would result in the inactivation of GSK 3β, which promotes cell growth by inhibiting apoptosis-related proteins including Bcl-2 family in 1, 2-dimethylhydrazine (DMH)-induced colorectal cancer (70).

In addition, the overexpression of GSK 3β and the nuclear transcription of NF-κB play critical roles in the survival and proliferation of pancreatic cancer cells (71). However, GSK 3β knockdown has been revealed to decrease the expression of Bcl-2 and VEGF, and ultimately result in the growth arrest of tumors (72). In addition, by increasing the expression of cyclin D1 and facilitating the proliferation of ovarian cancer cells, activation of GSK 3β could promote the activation of NF-κB and lead to cell cycle entering of tumor cells into the S phase (73). Accumulating evidence has revealed that GSK 3β could affect intracellular glucose metabolism by restraining the p53-, NF-κB- and c-Myc-mediated pathways, and thereafter promote the proliferation and survival of glioblastoma cells and protect them from apoptosis (20,74). Markedly, GSK 3β could transfer from the cytoplasm to the nucleus in glioblastoma (GBM) when stimulated by ionizing radiation (IR), and could bind to p53 binding protein 1 (53BP1) at Ser166. The binding of GSK 3β with 53BP1 results in the increased DNA double-strand break (DSB) repair in GBM after IR (75) (Fig. 5). Notably, GSK 3β inhibitor, SB216763, comparatively decreases the proliferation of GBM cells and induces apoptosis of GBM cells by halting DSB repair. Similarly, it has been revealed that GSK 3β inhibitor could block the NF-κB pathway and reduce the NF-κB-mediated transcription in osteosarcoma (OSA) (76).

Figure 5.

Molecular pathways revealing how GSK 3β influences tumor sensitivity to different chemo-therapeutic agents. In glioblastoma, GSK 3β inhibition improves temozolomide sensitivity by regulating the Mdm2/P53 and c-Myc/MGMT signaling pathways, which upregulate the methylation of MGMT promoter. Additionally, GSK 3β inhibitor enhances the sensitivity of pancreatic cancer to gemcitabine by negatively regulating the cyclin D1/CDK4/6 complex-dependent phosphorylation of Rb tumor suppressor protein. GSK 3β, glycogen synthase kinase 3β; DNMT3A, DNA methyltransferase 3 alpha; Mdm2, mouse double minute 2; MGMT, O⁶-methylguanine DNA methyl transferase; TP53INP1, tumor protein 53-induced-nuclear-protein 1; IR, ionizing radiation; 53BP1, p53 binding protein 1; CDK 4/6, cyclin-dependent kinase 4/6; Rb, retinoblastoma; E2F, E2 transcription factor.

However, as the present inhibitors are not specific but targeting both GSK 3α and GSK 3β, the oncogenic role of GSK 3β has been explored by gain and loss-of-function approaches of transgenic mice, including both knockout and knock-in animal models. A study by Kerkela et al revealed that the GSK 3β^−/− mice exhibited hypertrophic myopathy, which was caused by cardiomyocyte hyperproliferation with increased expression and nuclear localization of three important regulators of proliferation (GATA4, cyclin D1, and c-Myc) (77). Furthermore, Hoeflich et al reported that disruption of the GSK gene resulted in embryonic lethality caused by severe liver degeneration during mid-gestation with the activation of the transcription factor activation NF-κB, and the apoptotic sensitivity of GSK 3β^−/− fibroblasts was a direct consequence of GSK 3β deficiency (78). Using GSK 3β knock-in mice, studies have revealed that GSK-3 supports the maintenance of MLL leukemia cells by promoting continuous degradation of the cyclin-dependent kinase inhibitor p27Kip1 (79,80). In addition, phosphorylation of mTOR, p70S6K, and 4E-BP1 were impaired in GSK 3β knock-in mice (81). Therefore, GSK 3β can be perceived as an oncogenic protein and GSK 3β inhibitors are potential new drugs in cancer therapy.

4. Role of GSK 3β in oncotherapy

GSK 3β is also considered to be a pivotal mediator in regulating the sensitivity of tumor cells for chemotherapy and radiotherapy. It has been observed that the expression of GSK 3β is significantly increased in paclitaxel-resistant ovarian carcinoma cell line (SKOV3) (82). Moreover, downregulation of GSK 3β increased the therapeutic effect of 5-fluorouracil (5-FU), abolished cell viability and colony growth, and reduced the xenograft tumor mass in various drug-resistant p53-null colon cancer cell lines, which implies that GSK 3β inhibitor, in combination with chemotherapy, may represent a novel strategy for the treatment of chemotherapy-resistant tumors (83). GSK 3β inhibition was revealed to allow chemo-resistant carcinoma cells to become more susceptible to the synthetic multi-kinase inhibitor (sorafenib) which inhibits cell growth and angiogenesis (84). In addition, the increased expression of GSK 3β phosphorylation at Ser9 has been revealed to promote the chemo-sensitivity in cisplatin-resistant CP70 cells. The pGSK 3β (Ser9) increased the cisplatin resistance of ovarian carcinomas by reducing the GSK 3β regulated stabilization of p53 expression (85). Furthermore, LY294002, a PI3K inhibitor, has been revealed to enhance chemotherapy-induced apoptosis and the cleavage of caspase-8 by promoting GSK 3β dephosphorylation at Ser9 (86). Similarly, in MCF-7 breast cancer cells, selective inhibition of GSK 3β attenuated cytotoxicity induced by histone deacetylase inhibitor trichostatin A (87). Additionally, GSK 3β inhibition also enhanced the sensitivity of glioblastoma cells to radiation (75). In glioblastoma, GSK 3β inhibition improved the therapeutic effect of temozolomide by modulating the expression of O⁶-methylguanine DNA methyl transferase (MGMT) promoter via c-Myc signaling pathway (74,88). Moreover, GSK 3β inhibitor enhanced the sensitivity of pancreatic cancer to gemcitabine by decreasing the expression of tumor protein 53-induced-nuclear-protein 1 (TP53INP1), a pro-apoptotic stress-induced or DSB repair-mediated p53 target gene, and by downregulating the CDK4/6 complex-dependent phosphorylation of Rb tumor suppressor protein (89,90) (Fig. 5).

Collectively, GSK 3β regulates various responses to chemotherapy among a variety of tumor cell types, which is not entirely consistent with its role as a tumor suppressor or tumor promoter. On the one hand, GSK 3β inhibitor acts as a tumor promoter to attenuate the sensitivity of breast cancer cells to chemotherapy. On the other hand, GSK 3β functions as a tumor promoter for pancreatic cancer and GSK 3β inhibitor enhances the sensitivity of pancreatic cancer cells to chemotherapy. Therefore, the mechanisms of the GSK 3β signaling pathway in tumorigenesis and drug responses are significantly diverse, and require further investigations.

5. GSK 3β inhibitors in antineoplastic treatment

GSK 3β has been revealed to play a critical role in the regulation of a variety of cellular functions, including cell proliferation, differentiation, motility, apoptosis and the cell cycle (1,2,10). In addition, GSK3 inhibitors may be appropriate for the treatment of certain diseases, including diabetes, bipolar disorder, inflammation and certain types of cancer (91). Inhibitors of GSK 3β are categorized as GSK 3β-selective or non-selective and ATP-competitive or non-ATP-competitive (92–121).

Lithium, a well-documented inhibitor of GSK3, can promote phosphorylation of GSK 3β at Ser9 and inhibit the activity of GSK 3β by competing with magnesium (Mg²⁺) rather than ATP or its substrate. It has been indicated that lithium has become a gold standard for the treatment of bipolar mood and nervous disorders (92,93). Additionally, lithium has an effect on embryonic development by promoting the differentiation of epithelial cells, increasi calcngium (Ca²⁺) storage in the bones, and hindering the entering of the cell cycle (94–96). Lithium has been revealed to induce apoptosis in both Jurkat cells and differentiated immortalized hippocampal neurons by stimulating the death domain-containing receptor Fas (97). Notably, the treatment with lithium was revealed to increase the levels of p21^WAF/Cip1 (a protein with anti-apoptotic function) and survivin (a protein that supports the growth of cells by suppressing apoptosis and promoting cell proliferation) in human GBM cells (98). Furthermore, lithium was revealed to inhibit cell motility and compromise the invasive phenotype of v-Src-transformed cells, which was mediated by the activation of phospho-tyrosine phosphatases via the regulation of the cell redox status (99).

To date, several compounds targeting GSK 3 are still under pre-clinical studies, and few of them are in clinical trials (Table I). The mechanisms of GSK3 inhibition greatly vary. Both lithium and SB216763 have been demonstrated to have effects on tumor growth by inhibiting cell proliferation, however 9-ING-41 tends to induce apoptosis (100). SB216763, an ATP-competitive inhibitor of GSK 3β, has been revealed to downregulate AR-mediated prostate cancer cell growth in vitro and in vivo (101,102). It has also been revealed to potentiate the death ligand-induced apoptotic response in pancreatic cancer cells (PANC1 and MIA PaCa-2) by activating the c-Jun N-terminal kinase (JNK) pathway (103). Similarly, SB-415286, another ATP-competitive inhibitor specific for GSK 3β, was demonstrated to induce the expression of a β-catenin-LEF/TCF regulated reporter gene (104). Its treatment potentiated TRAIL- and CH-11-induced apoptosis in HepG2 cells, and induced growth arrest and apoptosis in multiple myeloma (MM) cells (105,106). Additionally, it decreased the viability of Neuro-2A cells, and induced G2/M arrest (107). Non-ATP competitive GSK 3β inhibitors, including LiCl, TDZD-8 and L803-mts, could also induce significant inhibition of tumor growth (108). A previous study has revealed that TWS119 could reverse the effects of tamoxifen on β-catenin and Snail expression thereby inhibiting GSK-3β/β-catenin activation (109). Furthermore, TWS119 exhibited different effects on CD4⁺ and CD8⁺ T cells in tumor-infiltrating lymphocytes (TILs) by stimulating the expansion of naive T cell and CD8 stem cell-like memory T cells, and inducing CD8⁺ effector T-cell proliferation in TILs (110). The selective GSK 3β inhibitor, manzamine A, can reduce the metastatic potential of AsPC-1 pancreatic cancer cells and promote TRAIL-induced cell apoptosis (111). Inhibition of GSK 3β with AR-A014418 was also revealed to increase the sensitivity of PANC1 cells to gemcitabine by regulating the Rb/E2F pathway (93) (112).

Table I.

Specific and non-specific GSK 3β inhibitors.

Compound	Specificity	Inhibition mode	Applications/mechanisms	(Refs.)
Lithium	Non-specific GSK 3β	Mg2+ competitive	Bipolar mood and nervous disorders	(96)
SB216763	Non-specific GSK 3β	ATP competitive	Inhibits AR-dependent reporter gene activity	(96–99)
SB415286	Non-specific GSK 3β	ATP competitive	Induces the accumulation of cells in the G2/M phase of the cell cycle and apoptosis in Neuro-2A cells	(100–103)
TDZD-8	Specific GSK3β	Non-ATP competitive	Activates the ERK pathway and increases expression of EGR-1 and p21 genes, which suppresses the proliferation of glioblastoma cells	(104)
L803-mts	Specific GSK3β	Non-ATP competitive	Significant inhibition of tumor growth in mouse xeno graft models of prostate cancer	(104)
TWS119	Specific GSK3β	ATP competitive	Decreases ATP production in cells, resulting in the increase in the AMP/ATP ratio and triggers AMPK in prostate cancer cells	(105 and 106)
Manzamine A	Specific GSK3β	Non-ATP-competitive	Inhibits autophagy and tumor growth	(107)
AR-A014418	Specific GSK3β	ATP competitive	Mainly suppresses the growth of pancreatic cancer by reducing the phosphorylation of GSK3α with concomitant Notch1 reduction	(86 and 108)
9-ING-41	Specific GSK3β	ATP-competitive	Inhibits the growth of various cancers and attenuates the progression of pulmonary fibrosis	(109–117)

GSK 3, glycogen synthase kinase 3; AR, androgen receptor; ERK, extracellular signal-regulated kinase; EGR-1, early growth response-1; AMP, adenosine monophosphate; ATP, adenosine triphosphate; AMPK, AMP-activated protein kinase.

Notably, 9-ING-41 [benzofuran-3-yl-(indol-3-yl)maleimide], a maleimide-based ATP-competitive small molecule GSK-3β inhibitor, has been demonstrated to be more selective for GSK 3β than for other related kinases (113). In vitro cell line studies and in vivo animal models have revealed the antitumor effects of 9-ING-41 in various cancers, including bladder cancer (114), renal cancer (115), neuroblastoma (116), B-cell lymphoma (117), breast cancer (118) and glioblastoma (119). A previous study on 9-ING-41 revealed that it upregulates the phosphorylation of the inhibitory serine residue of GSK 3β in ovarian cancer cells, and subsequently induces the apoptosis of ovarian cancer cells (120). A study from Ugolkov et al demonstrated that 9-ING-41 leads to the regression of patient-derived xenograft (PDX) tumors from metastatic pleural effusions obtained from patients with progressive, chemo-refractory breast cancer (118). Their group also revealed that 9-ING-41 significantly enhanced 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea (CCNU) antitumor activity in chemo-resistant PDX models of GBM by decreasing tumor size and increasing overall survival (119). In addition, 9-ING-41 could attenuate the progression of pulmonary fibrosis by improving lung functions and inhibition of myofibroblast differentiation in lung fibroblasts ex vivo and pulmonary fibrosis in vivo (121). With significant pre-clinical antitumor activity, 9-ING-41 is under phase 1/2 clinical trials in patients with refractory hematologic malignancies or solid Tumors (Clinical Trials ID: NCT03678883) (https://clinicaltrials.gov/ct2/show/NCT03678883).

6. GSK 3β in cancer immunotherapy

A previous study has revealed the immune-regulatory role of GSK 3β via the phosphorylation of two important transcription factors, NF-κB and CREB (122). Moreover, the phosphorylation of these two transcription factors could result in proteasomal degradation. In addition, the inactivation of GSK 3β has been revealed to lead to the nuclear transfer of NF-κB and CREB, which stimulate the secretion of inflammation-related cytokines (123,124).

For the innate immune system, the NK lymphocyte plays a critical role, and its activation relies on specific receptors including NKG2D/2B4 or NKG2D/DNAM-1. However, these activators may inhibit the activity of GSK 3β via the ERK or Akt signaling pathways (125,126). Studies on GSK 3β knockdown and its inhibitors revealed that the inactivation or silencing of GSK 3β leads to NK cell activation and enhanced function (127,128). A study on MM cells indicated that the inhibition of GSK3 upregulated MICA transcription and translation both in MM cell lines and in tumor cells isolated from MM patients, without significant effects on the basal expression of the MICB and DNAM-1 ligand poliovirus receptor/CD155. Moreover, GSK 3 inhibitors increased NK-mediated cytotoxicity of MM cells by activating NK cell degranulation. In addition, combined with lenalidomide or melphalan, treatment with GSK-3 inhibitors induced MICA expression and increased NK cell-mediated tumor killing by promoting NKG2D recognition in NK cells. Therefore, GSK-3 inhibitors could be novel therapeutic drugs targeting MICA expression and improving immune response in MM patients (129). For the acute myelogenous leukemia (AML) patients, NK cells expressed high levels of GSK 3β. Therefore, treatment with the GSK 3 inhibitors or the genetic inactivation of the GSK 3 led to the increased activity of NK cells to kill AML cells. Furthermore, GSK3 inhibition promoted the formation AML-NK cell conjugates by upregulating LFA expression on NK cells and by inducing ICAM-1 expression on AML cells (130). Another study on GSK 3 revealed that its inhibitor significantly upregulated transcription factors associated with late-stage NK-cell maturation, such as E-box binding homeobox 2 (ZEB2), PR/SET domain 1 (PRDM1), and T-box 21 (TBX21) and subsequently increased CD57 acquisition and maturation. In addition, NK cells, which were expanded ex vivo in the presence of GSK3 inhibitor, produced more TNF and IFN-γ, natural cytotoxicity and antibody-dependent cellular cytotoxicity (131). Therefore, currently, there are several ongoing clinical trials of adaptive NK cells treated with GSK 3 inhibitors for relapsed AML, ovarian cancer as well as other solid tumors.

Accumulating evidence has demonstrated the significance of GSK 3 in T cells. Because it is constitutively active in resting T cells, GSK 3 acts as a negative regulator of T-cell response by inhibiting CD8⁺ T-cell proliferation and IL-2 production (132). The inactivation of GSK 3 can specifically downregulate PD-1 expression by enhancing CD8⁺ CTL function and clearance of lymphoma cells (133,134). Additionally, GSK-3 inhibition was revealed to be as effective as anti-PD-1 and PD-L1 blocking antibodies in suppressing the growth of melanoma and lymphoma in mouse models (135). Therefore, GSK-3 was revealed as a central regulator of PD-1 expression. Recently, the same group revealed that GSK-3 also negatively regulated lymphocyte activation gene-3 (LAG-3) expression on CD4⁺ and CD8⁺ T cells. The combination of GSK-3 inhibitor with LAG-3 blockade resulted in the suppression of B16 melanoma growth and enhancement of tumor clearance, by increasing the expression of the transcription factor T-bet and binding with the LAG-3 promoter, and subsequently increasing granzyme B and interferon-γ1 expression (136). GSK 3β inhibition has been reported to increase the tumor cell cytotoxic capacity of CD8⁺ memory stem T cells in vitro against gastric cancer cells (137). Moreover, the inactivation of GSK 3β has been revealed to stimulate iTreg differentiation and increase the suppressive activity via the activation of the TGF-β/Smad3 signaling pathway (138). Furthermore, treatment of GBM-specific IL-13 CAR-T cells with the GSK 3 inhibitor resulted in reduced PD-1 expression, increased T cell survival and proliferation. Therefore, GSK 3 inactivation has also been revealed to contribute to the tumor treatment in CAR-T cell immunotherapy (139). Because the polarized-M2 phenotype of macrophages affects tumor growth, invasiveness, angiogenesis (140), and GSK 3β has been reported to enhance the polarization of microglia toward M2 (141), GSK 3β may be the potential therapeutic target for cancer immunotherapy. Collectively, these studies revealed that GSK 3 inhibition could be a new immunotherapeutic strategy for oncotherapy.

7. Conclusion

It has been widely acknowledged that GSK 3β is involved in tumorigenesis, cancer progression and metastasis. However, there are still controversies about the role of GSK 3β as a tumor suppressor or tumor promoter. GSK 3β may function as a tumor suppressor which inhibits neoplastic growth in prostate, oral and lung cancer when overexpressed or activated (51,142–144). Conversely, GSK 3β has also been revealed to facilitate carcinogenesis, and is recognized as a ‘tumor promoter’ in colon, pancreatic and ovarian cancers (65,89,90,120,145). Therefore, based on its pivotal role in tumorigenesis and tumor progression, GSK 3β is predicted to be a clinical prognostic indicator for certain malignant cancers. In conclusion, a more comprehensive understanding of GSK 3β would be comparatively beneficial to disease therapy. Targeting GSK 3β may be an ideal therapeutic strategy for malignant tumors that are characterized by infinite proliferation, metastasis and chemo-resistance, thus providing significant insight for the further research of tumor treatment.

Funding

The present study was supported by the National Natural Science Foundation of China (grant no. 81802504), the National Key Research and Development Program of China (grant no. 2020YFC2005500), the Key Research and Development Program of Science and Technology of the Department of Sichuan Province (grant no. 2019YFS0514) and the Sichuan Medical Association (grant no. Q19037).

Availability of data and materials

Data sharing is not applicable to this article, as no datasets were generated or analyzed during the present study.

Authors' contributions

YW conceived and designed the review. RH and SD wrote the paper. TL and XX prepared the figures and table. YW reviewed and edited the manuscript. All authors read and approved the manuscript, and agree to be accountable for all aspects of the research in ensuring that the accuracy or integrity of any part of the work are appropriately investigated and resolved.

Ethics approval and consent to participate

This review does not contain any studies with human participants or animals performed by any of the authors.

Patient consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.