Extended Data Figure 3. Genetic perturbation of Dlp and dally but not of SWIM alters the distribution of extracellular Wingless.

a) Expression of an RNAi against SWIM in the posterior compartment (en-Gal4) does not alter extracellular or total Wingless. Scale bar represents 50μm. b) Schematic representation of the SWIM locus and the CRISPR-Cas9 strategy used to delete the gene. Successful deletion was verified by PCR using two independent primer pairs indicated by purple and red arrowheads. c) Extracellular Wingless and Distal-less expression in control (w1118) and Swim^KO wing discs. Neither is altered upon SWIM deletion. Scale bars represent 50μm. d) Extracellular Wingless is reduced in clones lacking both Dlp and Dally (marked by the absence of GFP (white arrowheads). Scale bars represent 25μm. All experiments were repeated independently three times with similar results.