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. Author manuscript; available in PMC: 2021 Jul 7.
Published in final edited form as: Cytokine. 2021 Mar 13;142:155491. doi: 10.1016/j.cyto.2021.155491

Fig. 4. Transcriptional synergy at the IFN-λ3 promoter.

Fig. 4

A) Synergy between IRF3/7 and NF-κB p65 with RLR pathway stimulated (RLR +) or not (RLR-). The RLR pathway was stimulated when indicated by co-overexpression of HCV NS5B and RIG-I genes as described before (53). B) p65 enhances IRF7-mediated transcription at the IFN-λ3 promoter. Synergy between IRF7 and p65 at increasing concentration of IRF7 when p65 is held at a constant concentration (30 ng) is shown. C) No evidence of synergy between IRF3 and IRF7 either with or without (+/-) RLR pathway stimulation. D) CBP/p300 has no role in transcriptional synergy at the IFN-λ3 promoter. Synergy between IRF7 and p65 with the p1.4kbIFNL3 promoter clone is shown in presence or absence o of overexpressed p300. All experiments in A, B, C and D were done as three separate experiments with a single plasmid preparation. The results show mean values from the three replicates with error bars showing SD. The mean values are depicted near the curve or above the respective histograms in B, C and D. SV = synergy value defined as ratio of mean Luciferase expression value when both TFs are present together to that of the sum of mean Luciferase expression values when the two TFs are present individually. An SV of more than 1 suggests a synergistic cooperation between the TFs. All the Luciferase ratio values from each individual experiment are shown in Suppl. Table 1.