Extended Data Table 2. Cryo-EM data collection, refinement and validation statistics.
Cryo-EM data collection, refinement and validation statistics
| SmM Shutdown Heads Region (EMD-11069) (PDB 6Z47) |
SmM Shutdown Whole Molecule (EMD-11070) |
|
|---|---|---|
| Data collection and processing | ||
| Microscope | FEI Titan KRIOS | FEI Titan KRIOS |
| Detector | GATAN K2 | GATAN K2 |
| Voltage (keV) | 300 | 300 |
| Magnification | x 130,000 | x 130,000 |
| Total electron exposure (e–/Å2) | 60 | 60 |
| Exposure time (s) | 8 | 8 |
| Electron exposure per frame (e-/Å2) | 1.5 | 1.5 |
| Defocus range (μm) | -1.5 to -3.5 | -1.5 to -3.5 |
| Pixel size (Å) | 1.07 | 1.07 (3.21 for processing) |
| Symmetry imposed | C1 | C1 |
| Initial particle images (no.) | 329,910 | 90,914 |
| Final particle images (no.) | 96,351 | 52,713 |
| Map resolution (Å) | 6.3 | 9.0 |
| FSC threshold | 0.143 | 0.143 |
| Map resolution range (Å) | 2.9-12 | |
| Refinement | ||
| Initial model used (PDB code) | 1BR1, 2MYS, 1WDC, 3TS5 and beammotifCC | |
| Map sharpening B factor (Å2) | -362 | |
| Model composition | ||
| Non-hydrogen atoms | 23399 | |
| Protein residues | 2877 | |
| Ligands | 8 | |
| R.M.S.D from ideal geometry | ||
| Bond lengths (Å) | 0.007 | |
| Bond angles (°) | 1.062 | |
| Validation | ||
| MolProbity score | 1.23 | |
| Clashscore | 1.43 | |
| Poor rotamers (%) | 1.38 | |
| Ramachandran plot statistics | ||
| Favored (%) | 96.33 | |
| Allowed (%) | 3.42 | |
| Disallowed (%) | 0.24 | |