Antibodies
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InVivoMAb anti-mouse CD16/CD32 (Clone 2.4G2) |
Bioxcell |
Cat # BE0307 |
Fixable Viability Dye eFluor® 780 |
eBioscience |
Cat # 65-0865-18 |
Anti-mouse CD3e Biotin (Clone 145-2C11) |
BioLegend |
Cat# 100304 |
Anti-mouse CD5 Biotin (Clone 53-7.3) |
BioLegend |
Cat# 100604 |
Anti-mouse CD19 Biotin (Clone 6D5) |
BioLegend |
Cat# 115504 |
Anti-mouse Gr1 Biotin (Clone RB6-8C5) |
BioLegend |
Cat# 108404 |
Anti-mouse F4/80 Biotin (Clone BM8) |
BioLegend |
Cat# 123106 |
Anti-mouse CD11b Biotin (Clone M1/70) |
BioLegend |
Cat# 101204 |
Anti-mouse CD11c Biotin (Clone N418) |
BioLegend |
Cat# 117304 |
Anti-mouse NK1.1 Biotin (Clone PK136) |
BioLegend |
Cat# 108704 |
Anti-mouse TCRβ Biotin (Clone H57-597) |
BioLegend |
Cat# 109204 |
Anti-mouse TCRγδ Biotin (Clone eBioGL3) |
eBioscience |
Cat # 13-5711-85 |
Anti-mouse FceR1 Biotin (Clone MAR-1) |
BioLegend |
Cat# 134304 |
Anti-mouse TER-119 Biotin (Clone TER-119) |
BioLegend |
Cat# 116204 |
Anti-mouse CD45R/B220 Biotin (Clone RA3-6B2) |
BioLegend |
Cat# 103204 |
Anti-mouse Ly6G Biotin (Clone 1A8) |
BioLegend |
Cat# 127604 |
Streptavidin V500 |
BD Biosciences |
Cat# 561419 |
Anti-mouse Thy1.2 FITC (Clone 30-H12) |
BioLegend |
Cat# 105306 |
Anti-mouse Thy1.2 AF786 (Clone 30-H12) |
BioLegend |
Cat# 105331 |
Anti-mouse CD127/IL7Rα APC (Clone A7R34) |
BioLegend |
Cat# 135012 |
Anti-mouse CD127/IL7Rα BV605 (Clone A7R34) |
BioLegend |
Cat# 135041 |
Anti-mouse CD25 PE-Cy7 (Clone PC61) |
BioLegend |
Cat# 102016 |
Anti-mouse ICOS PE-Cy7 (Clone C398.4A) |
BioLegend |
Cat# 313520 |
Anti-mouse/human KLRG-1 FITC (Clone 2F1/KLRG1) |
BioLegend |
Cat# 138410 |
Anti-mouse/human KLRG-1 Percp-Cy5.5 (Clone 2F1/KLRG1) |
BioLegend |
Cat# 138417 |
Anti-mouse PD1/CD279 PE-Cy7 (Clone 29F.1A12) |
BioLegend |
Cat# 135216 |
Anti-mouse PD1/CD279 FITC (Clone 29F.1A12) |
BioLegend |
Cat# 135213 |
Anti-mouse CCR6 PE (29-2L17) |
BioLegend |
Cat# 129804 |
Anti-mouse CD103 FITC (2E7) |
BioLegend |
Cat# 121420 |
Anti-mouse CD45.1 FITC (Clone A20) |
BioLegend |
Cat# 110706 |
Anti-mouse CD45.2 BV711 (Clone 104) |
BioLegend |
Cat# 109847 |
Anti-mouse CD45.2 Pacific Blue (Clone 104) |
BioLegend |
Cat# 109820 |
Anti-mouse CD45 AF-700 (Clone 30-F11) |
BioLegend |
Cat# 110724 |
Anti-mouse CD3 AF-700 (Clone 17A2) |
BioLegend |
Cat# 100215 |
Anti-mouse CD3 BV711 (Clone 17A2) |
BioLegend |
Cat# 100241 |
Anti-mouse IL-5 PE (Clone TRFK5) |
BioLegend |
Cat# 504304 |
Anti-mouse IL-17A AF700 (Clone TC11-18H10.1) |
BioLegend |
Cat# 506914 |
Anti-mouse IL13 AF488 (Clone TWAJ) |
eBioscience |
Cat # 53-7133-82 |
Anti-mouse GATA-3 eF660 (Clone TWAJ) |
eBioscience |
Cat # 50-9966-42 |
Anti-mouse GATA-3 PerCP-eF710 (Clone TWAJ) |
eBioscience |
Cat # 46-9966-42 |
Anti-mouse Eomes AF488 (Clone Dan11mag) |
eBioscience |
Cat # 53-4875-82 |
Anti-mouse IL25R/IL17RB eF660 (Clone MUNC33) |
eBioscience |
Cat # 50-7361-82 |
Anti-mouse IL18Rα PE-Cy7 (Clone P3TUNYA) |
eBioscience |
Cat # 25-5183-82 |
Anti-mouse NKp46 / CD335 PercP-eF710 (29A1.4) |
eBioscience |
Cat # 46-3351-82 |
Anti-mouse/human TCF1/7 AF488 (Clone C63D9) |
Cell Signalling |
Cat # 6444S |
Anti-mouse IL33R/ST2 BV421 (Clone U29-93) |
BD Biosciences |
Cat# 566309 |
Anti-mouse RORγt PE (Clone Q31-378) |
BD Biosciences |
Cat# 562607 |
Anti-mouse RORγt AF647 (Clone Q31-378) |
BD Biosciences |
Cat# 562682 |
Anti-mouse RORγt BV650 (Clone Q31-378) |
BD Biosciences |
Cat# 564722 |
Anti-mouse PLZF PE (Clone Q31-378) |
BD Biosciences |
Cat# 564850 |
Anti-mouse Ki67 PerCP-Cy5.5 (Clone B56) |
BD Biosciences |
Cat# 561284 |
Anti-mouse CTLA-4 PE (Clone UC10-4B9) |
BD Biosciences |
Cat# 553720 |
Anti-mouse CD121b PE (Clone 4E2) |
BD Biosciences |
Cat# 554450 |
Anti- mouse ICOS (CD278) BUV395 (Clone 398.4A) |
BD Biosciences |
Cat# 565884 |
Anti-mouse CD69 BUV805 (Clone H1.2F3) |
BD Biosciences |
Cat# 741927 |
Anti-mouse KLRG-1 BUV661 (Clone 2F1) |
BD Biosciences |
Cat# 741586 |
Anti-mouse CD279 (PD-1) BUV737 (Clone RMP1-30) |
BD Biosciences |
Cat# 749306 |
Anti-mouse CD90.2 BU496 (Clone 30-H12) |
BD Biosciences |
Cat# 741047 |
Anti-mouse LPAM-1(a4b7) BV711 (Clone DATK32) |
BD Biosciences |
Cat# 740701 |
Anti-mouse CD103 BV786 (Clone M290) |
BD Biosciences |
Cat# 564322 |
Anti-mouse CD25 PE-Dazzle 594 (Clone PC61) |
BD Biosciences |
Cat# 562694 |
Anti-mouse CD3e PE-Cy5 (Clone 145-2C11) |
BD Biosciences |
Cat# 553065 |
Anti-mouse CD45.2 BUV737 (Clone 104) |
BD Biosciences |
Cat# 612778 |
Anti-mouse CD45 BUV496 (Clone 30-F11) |
BD Biosciences |
Cat# 749889 |
Anti-mouse IL33R/ST2 PE (Clone U29-93) |
BD Biosciences |
Cat# 566311 |
Anti-mouse CD90.2 BV605 (Clone 30-H12) |
BioLegend |
Cat# 140317 |
Anti-mouse CD45 BV510 (Clone 30-F11) |
BioLegend |
Cat# 103137 |
Anti-mouse CD45.2 FITC (Clone 104) |
BioLegend |
Cat# 109806 |
Anti-mouse CD45.1 Percp-Cy5.5 (Clone A20) |
BioLegend |
Cat# 110728 |
Anti-mouse CD4 PE-Texas Red (Clone RM4-5) |
eBioscience |
Cat # MCD0417 |
Anti-mouse CD8a PE-Texas Red (Clone 5H10) |
eBioscience |
Cat # MCD0817 |
Anti-mouse I-A/I-E AF-700 (Clone M5/114.15.2) |
BioLegend |
Cat# 107621 |
Anti-mouse TCRγδ Biotin (Clone GL3) |
BioLegend |
Cat# 118103 |
Anti-mouse Epcam Biotin (Clone G8.8) |
BioLegend |
Cat# 118203 |
Anti-mouse CD8α Biotin (Clone 53.67) |
BioLegend |
Cat# 100704 |
Anti-mouse CD3 Biotin (Clone 17A2) |
BioLegend |
Cat# 100243 |
Anti-mouse CD4 Biotin (Clone GK1.5) |
BioLegend |
Cat# 100404 |
Anti-mouse CD45 FITC (Clone 30-F11) |
BioLegend |
Cat# 103107 |
Anti-mouse NK1.1 PE-Cy7 (Clone PK136) |
BioLegend |
Cat# 108713 |
Anti-mouse CD19 FITC (Clone MB19-1) |
BioLegend |
Cat# 101505 |
Anti-mouse CD3e FITC (Clone 145-2C11) |
BD Bioscience |
Cat# 563061 |
Anti-mouse CD4 FITC (Clone H1 29.19) |
BD Bioscience |
Cat# 553651 |
Anti-mouse CD8a FITC (Clone 53-6.7) |
BD Bioscience |
Cat# 553031 |
Anti-mouse Gr1 FITC (Clone R86-8C5) |
BD Bioscience |
Cat# 553126 |
Anti-mouse Siglec-H FITC (Clone 551) |
BioLegend |
Cat# 129603 |
Anti-mouse B220 FITC (Clone RA3-6B2) |
BD Bioscience |
Cat#553088 |
Anti-mouse c-Kit/CD117 BV510 (Clone ACK2) |
BioLegend |
Cat#135119 |
Anti-mouse Sca-1 BV650 (Clone D7) |
BioLegend |
Cat#108143 |
Anti-mouse Flt3/CD135 PerCP–eFluor 710 (Clone A2F10) |
eBioscience |
Cat#46-1351-80 |
Anti-mouse CD27 PE (Clone LG3A10) |
BD Bioscience |
Cat# 558754 |
Anti-mouse Thy1.2 AF-700 (Clone 30-H12) |
BioLegend |
Cat# 105320 |
Anti-mouse ICOS BV785 (Clone C398.4A) |
BioLegend |
Cat# 313533 |
Anti-mouse IL18Rα AF647 (Clone BG/IL18RA) |
BioLegend |
Cat# 132903 |
Anti-mouse IL33R/ST2 (ST2) PE-Cy7 (Clone RMST2-2) |
eBioscience |
Cat#25-9335-82 |
Streptavidin BV510 |
Biolegend |
Cat#405233 |
Anti-mouse IL25R/IL17RB PE (Clone 9B10) |
BioLegend |
Cat#146305 |
Anti-mouse CD127/IL7Rα BV421 (Clone A7R34) |
BD Biosciences |
Cat# 566377 |
Anti-mouse TCRγδ Antibody APC (Clone GL3) |
BioLegend |
Cat#118115 |
Anti-mouse/human CD11b FITC (Clone M1/70) |
eBioscience |
Cat# 11-0112-82 |
Anti-mouse TER-119 FITC (Clone TER-119) |
eBioscience |
Cat# 11-5921-82 |
Anti-human CD14 APC-eF780 (Clone 61D3) |
eBioscience |
Cat# 47-0149-42 |
Anti-human CD19 APC-eF780 (Clone HIB19) |
eBioscience |
Cat# 47-0199-42 |
Anti-human CD123 APC-eF780 (Clone 6H6) |
eBioscience |
Cat# 47-1239-42 |
Anti-human CD3 APC-eF780 (Clone SK7) |
eBioscience |
Cat# 47-0038-42 |
Anti-human FceRIa APC Vio770 (Clone CRA1) |
Miltenyi |
Cat# 130-105-500 |
Anti-human CD127 PE (Clone MB15-18C9) |
Miltenyi |
Cat# 130-113-410 |
Anti-human CD218(IL18Ra) PEVio770 (Clone H44) |
Miltenyi |
Cat# 130-101-723 |
Anti-human ICOS APC (Clone REA192) |
Miltenyi |
Cat# 130-101-723 |
Anti-human EOMES FITC (Clone WD1928) |
eBioscience |
Cat# 11-4877-42 |
Anti-human CRTH2 PerCP-Cy5.5 (Clone BM16) |
BioLegend |
Cat# 350116 |
Anti-mouse/human T-bet BV605 (Clone 4B10) |
BioLegend |
Cat# 644817 |
Anti-human CD117(c-KIT) BV650 (Clone 104D2) |
BioLegend |
Cat# 313222 |
Anti-human CD103 BV711 (Clone Ber-ACT8) |
BioLegend |
Cat# 350222 |
Anti-human CD336(NKP44) Biotin (Clone P44-8) |
BioLegend |
Cat# 325106 |
Streptavidin BV785 |
BioLegend |
Cat# 405249 |
Experimental Models: Organisms/Strains
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Mouse: wild-type: |
C57BL/6, Charles River Laboratories |
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Mouse: CD45.1/Ly5.1 |
B6.SJL-Ptprca Pepcb/BoyJ, Jax mice |
JAX stock #006584 |
Mouse: Ubiquitin
tdT (“Ubow”) |
(Ghigo et al., 2013) |
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Mouse: IL5cre x LSLtdTom |
(Nussbaum et al, 2013) |
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Mouse: RAG1 -/-
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B6.129S7-Rag1tm1Mom/J |
JAX stock #002216 |
Mouse: RAG2-/- γc-/-
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C57BL/6NTac;B10(Cg)-Rag2tm1Fwa Il2rgtm1Wj1, Taconic, 4111-F |
Software and Algorithms
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FlowJo v.10.5.0 |
FlowJo |
https://www.flowjo.com
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Prism v.6.0. |
GraphPad |
https://www.graphpad.com
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R v. 3.5.1 and 3.6.1 |
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https://www.r-project.org/
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