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. Author manuscript; available in PMC: 2022 Feb 28.
Published in final edited form as: Oncogene. 2022 Jan 29;41(8):1216–1224. doi: 10.1038/s41388-021-02162-0

Figure 1. Overexpression of CDC25A, CCNE1 or MYC leads to replication stress.

Figure 1

(A) Indicated RPE1-TP53 wt cell lines were treated with doxycycline for 48 hours. Immunoblotting was performed for CDC25A, CCNE1, p53, and β-Actin. (B) Cells were treated with doxycycline as described for panel A and subsequently pulse-labeled for 20 minutes with CldU (25 μM) and 20 minutes with IdU (250 μM). Representative DNA fibers from RPE1-TP53 wt cells are shown. Scale bar represents 10 μm. (C-G) Quantification of IdU DNA fiber lengths, as described in panel B. Per condition, 300 fibers from RPE1-TP53 wt cells were analyzed. P values were calculated using the Mann-Whitney U test for RPE1-TP53 wt (panel C), RPE1-TP53 mut (panel D), MDA-MB-231 (panel E), BT549 (panel F) and HCC1806 (panel G).