Skip to main content
. Author manuscript; available in PMC: 2022 Jun 7.
Published in final edited form as: Memo. 2022 Jan 21;15(2):137–142. doi: 10.1007/s12254-021-00790-6

Fig. 2. The KinCon biosensor concept.

Fig. 2

a Modular structure of the KinCon biosensor. Mammalian expression vectors encode for the full-length kinase sequence and flanked fragments of the luciferase PCA. Exemplarily, we show that the chosen full-length kinase contains one cis regulatory element (CRE). A flexible linker separates fragment 1 (–F[1]) and fragment 2 (–F[2]) of the luciferase PCA. b The opened and active full-length kinase conformation is adopted when –F[1] and –F[2] of the PCA-luciferase are spatially separated. In the presence of the respective luciferase substrate less or no bioluminescence is emitted. Conventionally, in a more closed kinase conformation, the kinase is less active or inactive. Thus, the two fragments are in close proximity to form a complemented and functional luciferase which catalyzes substrate conversion and consequently recordable light emissions. The different means leading to KinCon dynamics are discussed in relation to Fig. 3