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. Author manuscript; available in PMC: 2024 Feb 1.
Published in final edited form as: Nature. 2023 Aug 2;620(7973):402–408. doi: 10.1038/s41586-023-06372-2

Extended data 1. Strategy to study the impact of Netrin-1 on EMT in mouse skin SCCs.

Extended data 1

a, Mouse model of skin SCC allowing the expression of KrasG12D, YFP, p53 deletion and overexpression of human NETRIN-1 in hair follicle stem cells and their progeny using Lgr5CreER. b, Relative mRNA expression of NTN1 in EPCAM-control LKPR (n = 5) and LKPR-NTN1 (n = 8) skin SCC defined by qRT-PCR (data are normalized to Tbp gene, mean ± s.e.m., two tailed Mann-Whitney U test). c, Western blot analysis of Netrin-1 expression in EPCAM-control LKPR and LKPR-NTN1 skin SCC TCs. d, FACS plots showing the gating strategy used to FACS-isolate or to analyse the proportion of YFP+/ EPCAM+ and EPCAM− tumour cells. e, Drawing showing the experimental strategy of NP137 administration after Tamoxifen induction in Lgr5CreER/ KrasLSL-G12D/p53 fl/fl/Rosa26-YFP+/+ mice. IP, intraperitoneal.