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. Author manuscript; available in PMC: 2023 Dec 13.
Published in final edited form as: Chem. 2023 Feb;9(2):523–540. doi: 10.1016/j.chempr.2022.11.005

Figure 1. Fixation-driven chemical cross-linking of exogenous ligands (FixEL) in the mouse brain.

Figure 1

(A) The contribution of hydrogel-tissue chemistry (HTC) and a remaining challenge.

(B) Schematic illustration of protein cross-linking by PFA.

(C) Schematic illustration of the FixEL strategy. FL and Lg refer to fluorophore and ligand, respectively.

(D) FixEL probes used in this study. Probe 1 (mGlu1) has a CNITM moiety, which is a ligand for mGlu1, and an amino group. Probe 2 (AMPAR) has a PFQX moiety, which is a ligand for AMPAR, and an amino group. Probe 3 (DRD2) has a spiperone moiety, which is a ligand for DRD2, and an amino group.